UltraCruz® Blocking Reagent Plant protein derived universal antibody diluent and blocking reagent recommended for use in immunohistochemistry (IHC), immunofluorescence (IF), Western blotting (WB), and for other research applications.

UltraCruz® Blocking Reagent: sc-516214

UltraCruz Blocking Reagent is rated 4.0 out of 5 by 1.
  • y_2019, m_2, d_14, h_17
  • bvseo_bulk, prod_bvrr, vn_bulk_2.0.11
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_1
  • loc_en_US, sid_516214, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 279ms
  • REVIEWS, PRODUCT
Datasheets
    • 100 ml ready-to-use solution for blocking and dilution
    • Plant protein derived universal antibody diluent and blocking reagent recommended for use in immunohistochemistry (IHC), immunofluorescence (IF), Western blotting (WB), and for other research applications.
    • animal-free alternative to sera, BSA or non-fat dry milk
    • Designed to stabilize diluted antibody solutions and minimize background due to non-specific binding in different antibody-based research applications
    • The UltraCruz® Blocking Reagent is especially convenient in multiplex IHC (multiple antigen labeling) applications where antibodies from several different species are used
    • Highly recommended for use with ImmunoCruz® Antibody Conjugates and for Near-Infrared (NIR) Western blot detection methods, such as LI-COR/Odyssey® and other comparable systems
Submit a review for this product and receive 15 CruzCredits

Dear sir  I am interested in your secondaries antibodies, but I knew from your website that your secondaries were not prepared in any live host, therefore there is high specificity and minimal background. Is there no need to use the blocking reagent

Asked by: Doaa
Thank you for your question. We still recommend using a blocking reagent for your research applications even when using our recombinant mouse IgG binding proteins in lieu of a conventional secondary antibody that is derived from a living host animal.
Answered by: Technical Support
Date published: 2017-12-19

Do I need to add detergent (exple: Triton X-100) to solubilize the membrane as with conventional Blocking sera to the UltraCruz® Blocking Reagent: sc-516214?

Asked by: Flo81
Thank you for your question. Yes, UltraCruz® Blocking Reagent: sc-516214 is a plant-based alternative to conventional blocking serums or buffers. It can be used in place of other blocking reagents. But if you typically use Triton X-100 in your protocols, then we would recommend to continue to do so as this buffer should not interfere with that procedure.
Answered by: Technical Support
Date published: 2018-07-12

Does UltraCruz® Blocking Reagent (sc-516214) contain glycine? What are the ingredients of this product? The datasheed does not provide any information. Thank you.

Asked by: Stark
Thank you for your question. Our UltraCruz® Blocking Reagent: sc-516214 is a proprietary buffer that is an animal-free alternative to sera, BSA or non-fat dry milk. The ingredients in the buffer are not provided as this is proprietary information. Please contact our Technical Service Department if you require additional information to purchase, or use this blocking buffer. Please call our European Support office toll-free at 00800 4573 8000, email europe@scbt.com, or contact us by live chat.
Answered by: Technical Support
Date published: 2017-05-03

What's the concentration I should use for blocking knowing that I will use secondary antibody m-IgGκ BP: sc-516102? Thank you

Asked by: toujj
Thank you for your question. Our UltraCruz® Blocking Reagent: sc-516214 is provided as a 1X 100 ml ready-to-use solution for blocking and dilution. There is no need to further dilute the blocking reagent. You can use as much is needed to cover your samples, or dilute your primary and secondary (sc-516102) reagents. A link to our recommended protocol for immunohistochemistry is provided below for your reference. https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2018-07-12
  • y_2019, m_2, d_14, h_17CST
  • bvseo_bulk, prod_bvqa, vn_bulk_2.0.11
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_4
  • loc_en_US, sid_516214, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 42ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from good products I bought the blocking reagent and is a good product making the research easier.
Date published: 2017-03-22
  • y_2019, m_2, d_14, h_17
  • bvseo_bulk, prod_bvrr, vn_bulk_2.0.11
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_1
  • loc_en_US, sid_516214, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 5ms
  • REVIEWS, PRODUCT

Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2019 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.