U-73122 is an inhibitor of phospholipase C, phospholipase A2, and 5-LO (5-lipoxygenase). In neutrophils and human platelets, U-73122 inhibits agonist-induced phospholipase C (IC50=1.0-2.1 µM) activation. This compound affects the phospholipases by inhibiting the hydrolysis of PPI (phosphatidylinositol) to IP3 (inositol triphosphate), which in turn leads to a drop in cytosolic Ca2+. In SK-N-SH neuroblastoma cells, U-73122 inhibits agonist-induced down-regulation of muscarinic receptors. In addition, it is a useful tool to investigate receptor-mediated PI turnover in signal transduction. U-73122 is a potent inhibitor of human neutrophil adhesion to biological surfaces (IC50 = 50 nM) as well as adhesion-dependent granule exocytosis and oxidative burst. U-73343 (sc-201422) is useful as a negative control for investigations of U-73122 phospholipase C antagonism and its cellular consequences.
1. Smith, R.J., et al. 1990. J. Pharmacol. Exp. Ther. 253: 688-697. PMID: 2338654
2. Bleasdale, J.E., et al. 1990. J. Pharmacol. Exp. Ther. 255 756-768. PMID: 2147038
3. Thompson A.K., et al. 1991. J. Biol. Chem. 266: 23856-23862. PMID: 1660886
4. Yule, D.I., et al. 1992. J. Biol. Chem. 267: 13830-13835. PMID: 1629184
5. Smith, R.J., et al. 1996. J. Pharmacol. Exp. Ther. 278: 320-329. PMID: 8764366
See how others have used U-73122. Click on the entry to view the PubMed entry .
PMID: # 26673139
Galindo-Villegas, J. et al. 2016. TRPV4-Mediated Detection of Hyposmotic Stress by Skin Keratinocytes Activates Developmental Immunity. J. Immunol.. 196: 738-49.
PMID: # 27127172
Kamranvar, SA. et al. 2016. Integrin signaling via FAK-Src controls cytokinetic abscission by decelerating PLK1 degradation and subsequent recruitment of CEP55 at the midbody. Oncotarget. 7: 30820-30.
PMID: # 26185418
Khan, N. et al. 2015. Investigation of cyclooxygenase and signaling pathways involved in human platelet aggregation mediated by synergistic interaction of various agonists. Drug design, development and therapy. 9: 3497-506.
PMID: # 25277212
Leung, CS. et al. 2014. Calcium-dependent FAK/CREB/TNNC1 signalling mediates the effect of stromal MFAP5 on ovarian cancer metastatic potential. Nature communications. 5: 5092.
PMID: # 25277212
Leung, CS. et al. 2014. Calcium-dependent FAK/CREB/TNNC1 signalling mediates the effect of stromal MFAP5 on ovarian cancer metastatic potential. Nat Commun. 5: 5092.
PMID: # 24630454
Dew, WA. et al. 2014. Contaminant-specific targeting of olfactory sensory neuron classes: connecting neuron class impairment with behavioural deficits. Chemosphere. 112: 519-25.
PMID: # 24357048
Luo, XP. et al. 2014. Phospholipase C ε-1 inhibits p53 expression in lung cancer. Cell biochemistry and function. 32: 294-8.
PMID: # 24420748
Xiao, J. et al. 2014. Regulation of chondrocyte proliferation through GIT1-Rac1-mediated ERK1/2 pathway by PDGF. Cell biology international. 38: 695-701.
PMID: # 25175053
Xiao, J. et al. 2014. PDGF regulates chondrocyte proliferation through activation of the GIT1- and PLCγ1-mediated ERK1/2 signaling pathway. Molecular medicine reports. 10: 2409-14.
PMID: # 23411401
Sooampon, S. et al. 2013. Thermal stimulation of TRPV1 up-regulates TNFα expression in human periodontal ligament cells. Archives of oral biology. 58: 887-95.
Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2017 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.