Tryptone is an enzymatic digest of casein used as a nitrogen source in culture media. Casein is the main protein of milk, and a rich source of amino-acid nitrogen. Tryptone is rich in tryptophane, making it valuable for use in detecting indole production. The absence of detectable levels of carbohydrates in Tryptone makes it a suitable peptone in differentiating bacteria on the basis of their ability to ferment various carbohydrates.
1. Jay, J.M. 1971. Infect Immun. 3 :544-547. PMID: 16558014
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