Trypan Blue A viability dye for quantifying live versus dead cells

Trypan Blue (CAS 72-57-1)

Trypan Blue | CAS 72-57-1 is rated 5.0 out of 5 by 1.
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Synonym: Direct Blue 14; Dianil blue; Diaminine Blue
Application: A viability dye for quantifying live versus dead cells
CAS Number: 72-57-1
Molecular Weight: 960.81
Molecular Formula: C34H24N6O14S44Na
* Refer to Certificate of Analysis for lot specific data (including water content).
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Trypan Blue is an azo based, hydrophilic, tetrasulfonated blue acid dye widely utilized for assessing cell viability. As an easy method to determining the viability of cells, Trypan Blue will stain dead cells with permeable membranes blue, while the dye is excluded by most living cells and their intact membranes thereby allowing visual determination of living versus dead cells. Although Trypan Blue is predominately used for assessing cell viability of cultured cells, other applications for this dye have been reported. In rat abdominal organ slices Trypan Blue has been utilized to determine cell viability as well as islet cell cluster cell viability from caprine pancreatic samples.


References

1. Bonnefille, P. et al. 2010. Fundam Clin Pharmacol. PMID: 21121944
2. Han, T.H. et al. 2010. J Acupunct Meridian Stud. 3(4): 241-248. PMID: 21185538
3. Saadatnia, G. et al. 2010. Trop Biomed. 27(1): 125-130. PMID: 20562822
4. Hani, H. et al. 2010. Xenotransplantation. 17(6): 469-480. PMID: 21158948

Appearance :
Powder
Physical State :
Solid
Solubility :
Soluble in water (10 mg/ml at 25° C), ethanol (0.6 mg/ml), and EGME (20 mg/ml).
Storage :
Store at 4° C
Melting Point :
>300° C (lit.)
IC50 :
SH-PTP2: IC50 = 3.9 µM (human); Protein-tyrosine phosphatase 1: IC50 = 7.4 µM (Saccharomyces cerevisiae); P2Y2: IC50 = 17.1 µM (human)
pK Values :
pKa: -1.53 (Predicted), pKb: 2.39 (Predicted)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
RTECS :
QJ6475000
PubChem CID :
9562061
Merck Index :
14: 9792
MDL Number :
MFCD00003969
EC Number :
200-786-7
Beilstein Registry :
4360496
SMILES :
CC1=C(C=CC(=C1)C2=CC(=C(C=C2)NN=C3C(=CC4=CC(=CC(=C4C3=O)N)S(=O)(=O)[O-])S(=O)(=O)[O-])C)NN=C5C(=CC6=CC(=CC(=C6C5=O)N)S(=O)(=O)[O-])S(=O)(=O)[O-].[Na+].[Na+].[Na+].[Na+]

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Certificate of Analysis

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Trypan Blue  Product Citations

See how others have used Trypan Blue. Click on the entry to view the PubMed entry .

Citations 1 to 6 of 6 total

PMID: # 26011702  Li, L. et al. 2016. J Appl Toxicol. 36: 257-65.

PMID: # 25778668  Dong, F. et al. 2015. Int. J. Mol. Med. 35: 1381-7.

PMID: # 25944692  Keerthikumar, S. et al. 2015. Oncotarget. 6: 15375-96.

PMID: # 25915824  Jeong, D. et al. 2015. Leukemia. 29: 1868-74.

PMID: # 24909688  Dong, F. et al. 2014. Biochem. Biophys. Res. Commun. 450: 447-52.

PMID: # 25206547  Shu, X. et al. 2013. Neural regeneration research. 8: 2370-8.

Citations 1 to 6 of 6 total
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Rated 5 out of 5 by from HeLa cells were used to demonstrate the functionality HeLa cells were used to demonstrate the functionality of Trypan Blue. This cell culture was exposed to puromycin dihydrochloride or geneticin (G418) sulfate for 24/48 hours respectively, and then tested with Trypan Blue. Trypan Blue was used to demonstrate cell viability. Dead cells will take in the dye, while live cells with intact membranes will exclude this dye. -SCBT QC
Date published: 2015-03-21
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