Tris, Hydrochloride (Molecular Biology Grade) is commonly used as a buffer component of phenol extraction of DNA or RNA and a buffering component of separating and stacking gels in the characterization of protein products by SDS-PAGE. Tris, Hydrochloride has also been used with urea as a method for antigen retrieval in immunohistochemistry. In smooth muscles Tris, Hydrochloride has been observed to inhibit motor responses to adrenergic motor nerve stimulation. In the crystalization of porcine pancreatic elastase with use of Tris, Hydrochloride, the Tris, Hydrochloride buffer was observed to induce conformational change and crystal-packing contraction. Tris, Hydrochloride has also been incorporated in coelomic fluid and in Cortland medium to store unfertilized rainbow trout eggs.
DNases, proteases, RNases: none detected; heavy metals: ≤0.0002% (as Pb). Absorbance (260 nm and 280 nm): ≤0.06
1. Jagus, R., 1987. Meth. Enzymol. 152 : 296-304. PMID: 3657575 2. Wallace, D.M. 1987. Meth. Enzymol. 152: 33-41. PMID: 2443801 3. Kinoshita, T., et al. 2006. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 62: 623-626. PMID: 16820677 4. Gillespie, J.S., et al. 1976. J. Physiology. 259: 561-573. 5. Goetz, F., et al. 2000. Aquaculture. 184 : 267-276.
Soluble in water (4 M) at 20 °C.
Useful in the range of 7.0-9.0.
Store at room temperature
150-152° C (dec.)
357° C at 760 mmHg (Predicted)
1.05 g/cm3 at 20° C
pKa: 8.1(25° C)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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Se puede usar para preservar tejidos que serán usados para HPLC?
Asked by: citla
Gracias por tu pregunta. Tris HCL se usa comúnmente como una disolución reguladora de la extracción con fenol de ADN o ARN y un componente amortiguador para separar y apilar geles en la caracterización de productos de proteínas por SDS-PAGE. Desconocemos que se utilice para preservar tejidos.
Thank you for your question. Tris HCL is commonly used as a buffer component of phenol extraction of DNA or RNA and a buffering component of separating and stacking gels in the characterization of protein products by SDS-PAGE. We are unaware of it being used to preserve tissues.
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