Tris, Hydrochloride (Molecular Biology Grade)A high quality buffer component used in SDS-PAGE

Tris, Hydrochloride (Molecular Biology Grade) (CAS 1185-53-1)

  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 75ms
  • REVIEWS, PRODUCT
  • bvseo-msg: The resource to the URL or file is currently unavailable.;
Synonym: TRIS hydrochloride; Tris(hydroxymethyl)aminomethane hydrochloride
Application: A high quality buffer component used in SDS-PAGE
CAS Number: 1185-53-1
Purity: ≥99%
Molecular Weight: 157.6
Molecular Formula: C4H11NO3HCl
* Refer to Certificate of Analysis for lot specific data (including water content).
Submit a review for this product and receive 15 CruzCredits

Tris, Hydrochloride (Molecular Biology Grade) is commonly used as a buffer component of phenol extraction of DNA or RNA and a buffering component of separating and stacking gels in the characterization of protein products by SDS-PAGE. Tris, Hydrochloride has also been used with urea as a method for antigen retrieval in immunohistochemistry. In smooth muscles Tris, Hydrochloride has been observed to inhibit motor responses to adrenergic motor nerve stimulation. In the crystalization of porcine pancreatic elastase with use of Tris, Hydrochloride, the Tris, Hydrochloride buffer was observed to induce conformational change and crystal-packing contraction. Tris, Hydrochloride has also been incorporated in coelomic fluid and in Cortland medium to store unfertilized rainbow trout eggs.

DNases, proteases, RNases: none detected; heavy metals: ≤0.0002% (as Pb). Absorbance (260 nm and 280 nm): ≤0.06


References

1. Jagus, R., 1987. Meth. Enzymol. 152 : 296-304. PMID: 3657575
2. Wallace, D.M. 1987. Meth. Enzymol. 152: 33-41. PMID: 2443801
3. Kinoshita, T., et al. 2006. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 62: 623-626. PMID: 16820677
4. Gillespie, J.S., et al. 1976. J. Physiology. 259: 561-573.
5. Goetz, F., et al. 2000. Aquaculture. 184 : 267-276.

Appearance :
Crystalline
Physical State :
Solid
Solubility :
Soluble in water (4 M) at 20 °C.
pH :
Useful in the range of 7.0-9.0.
Storage :
Store at room temperature
Melting Point :
150-152° C (dec.)
Boiling Point :
357° C at 760 mmHg (Predicted)
Density :
1.05 g/cm3 at 20° C
pK Values :
pKa: 8.1(25° C)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
1
RTECS :
TY2900000
PubChem CID :
93573
Merck Index :
12: 9902
MDL Number :
MFCD00012590
EC Number :
214-684-5
Beilstein Registry :
3675235
SMILES :
C(C(CO)(CO)N)O.Cl

Download SDS (MSDS)

Certificate of Analysis

Adobe Acrobat Reader is required to reliably view,
print and comment on PDF documents

Se puede usar para preservar tejidos que serán usados para HPLC?

Asked by: citla
Gracias por tu pregunta. Tris HCL se usa comúnmente como una disolución reguladora de la extracción con fenol de ADN o ARN y un componente amortiguador para separar y apilar geles en la caracterización de productos de proteínas por SDS-PAGE. Desconocemos que se utilice para preservar tejidos. Thank you for your question. Tris HCL is commonly used as a buffer component of phenol extraction of DNA or RNA and a buffering component of separating and stacking gels in the characterization of protein products by SDS-PAGE. We are unaware of it being used to preserve tissues.
Answered by: Technical Support
Date published: 2018-12-31
  • y_2019, m_8, d_20, h_6CST
  • bvseo_bulk, prod_bvqa, vn_bulk_2.0.13
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_en_US, sid_338743, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 106ms
  • QUESTIONS, PRODUCT
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 34ms
  • REVIEWS, PRODUCT
  • bvseo-msg: The resource to the URL or file is currently unavailable.;

Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2019 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.