Tris, Hydrochloride A high quality buffer component used in SDS-PAGE

Tris, Hydrochloride (CAS 1185-53-1)

Tris, Hydrochloride | CAS 1185-53-1 is rated 5.0 out of 5 by 1.
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Synonym: Tris(hydroxymethyl)aminomethane hydrochloride; TRIS HCl; Trizma® hydrochloride
Application: A high quality buffer component used in SDS-PAGE
CAS Number: 1185-53-1
Purity: ≥99%
Molecular Weight: 157.6
Molecular Formula: C4H11NO3•HCl
* Refer to Certificate of Analysis for lot specific data (including water content).
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Tris, Hydrochloride is commonly used as a buffer component for phenol extractions of DNA or RNA and a buffering component of separating and stacking gels in the characterization of protein products by SDS-PAGE. Tris, Hydrochloride has also been used with urea as a method for antigen retrieval in immunohistochemistry. In smooth muscles Tris, Hydrochloride has been observed to inhibit motor responses to adrenergic motor nerve stimulation. In the crystalization of porcine pancreatic elastase with use of Tris, Hydrochloride, the Tris, Hydrochloride buffer was observed to induce conformational change and crystal-packing contraction. Tris, Hydrochloride has also been incorporated in coelomic fluid and in Cortland medium to store unfertilized rainbow trout eggs.


References

1. Jagus, R. 1987. Meth. Enzymol. 152 : 296-304. PMID: 3657575
2. Wallace, D.M. 1987. Meth. Enzymol. 152 : 33-41. PMID: 2443801
3. Kinoshita, T., et al. 2006. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 62: 623-626. PMID: 16820677
4. Gillespie, J.S., et al. 1976. J. Physiol. 259: 561-573. PMID: 182965

Appearance :
Crystalline
Physical State :
Solid
Solubility :
Soluble in water (667 mg/ml).
Storage :
Store at room temperature
Melting Point :
150-152° C
Boiling Point :
357° C at 760 mmHg (Predicted)
pK Values :
pKa: pK1: 8.3(20° C), pK1: 7.82(37° C), pK2: 8.08(25° C)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
1
PubChem CID :
93573
MDL Number :
MFCD00012590
EC Number :
214-684-5
Beilstein Registry :
3675235
SMILES :
C(C(CO)(CO)N)O.Cl

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Certificate of Analysis

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Is this soluble in PBS? Wants to make a 5mM solution with a pH of 7.6.?

Asked by: Jz28sail
Thank you for your question. Yes, sc-216106, Tris, Hydrochloride is soluble in aqueous buffers. Alternatively, we offer sc-301951, Tris hydrochloride solution, pH 7.6 as a 1M solution ready to use.
Answered by: Chemical Support 5
Date published: 2017-04-05
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Rated 5 out of 5 by from Various publications cite the use of Tris Various publications cite the use of Tris as a buffer component for phenol extractions of DNA or RNA and a buffering component of separating and stacking gels in the characterization of protein products by SDS-PAGE. -SCBT Publication Review
Date published: 2015-05-07
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