TRIS Glycine buffer solution 10X, for electrophoretic transfer of protein from polyacrylamide gels to nitrocellulose sheets

TRIS Glycine buffer solution

TRIS Glycine buffer solution is rated 5.0 out of 5 by 1.
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Application: 10X, for electrophoretic transfer of protein from polyacrylamide gels to nitrocellulose sheets
* Refer to Certificate of Analysis for lot specific data (including water content).

TRIS Glycine buffer solution, also called Running buffer and similar to Towbin buffer, is supplied as 1L of 10X concentrate that can be diluted to a 1X solution containing 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3. Researchers may check and adjust the final pH using a sensitive pH meter.
Also available:
TG-SDS Buffer, 10X Powder Pack (sc-296527)


References

1. Towbin, H., et al. 1979. Proc. Natl. Acad. Sci. U.S.A. 76: 4350-4354. PMID: 388439<br>

Formulation :
10X
pH :
8.3 &plusmn;0.2 (1X)
Storage :
Store at 4° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Download SDS (MSDS)

Certificate of Analysis

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Rated 5 out of 5 by from Various publications cite the use of TRIS Various publications cite the use of TRIS Glycine buffer solution or Towbin buffer for electrophoretic transfer of protein from polyacrylamide gels to nitrocellulose sheets. -SCBT Publication Review
Date published: 2015-07-13
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