Tris-EDTA buffer solution, 100X Used to solubilize and protect DNA and RNA

Tris-EDTA buffer solution, 100X

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Application: Used to solubilize and protect DNA and RNA
* Refer to Certificate of Analysis for lot specific data (including water content).
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Tris-EDTA Buffer Liquid is a 100X concentrate that, when diluted to 1X, contains 10 mM Tris, 1 mM EDTA, pH ~8.0. Based on nuclease studies from the 1980's, the pH is usually adjusted to 7.5 for RNA and 8.0 for DNA. The respective DNA and RNA nucleases are supposed to be less active at these pH values, but pH 8.0 can safely be used for storage of both DNA and RNA. EDTA further inactivates nucleases, by binding to metal ions required by these enzymes.

pH :
~8.0
Storage :
Store at room temperature
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

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Certificate of Analysis

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Is this buffer molecular grade?

Asked by: kassi
Thank you for your question. Our Tris-EDTA buffer solution, 100X is made with DEPC treated MQ water. It has been filtered via a 0.22 µm filter.
Answered by: Technical Support
Date published: 2017-09-21
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