Tris Buffered Saline with Tween 20: powder for 100 ml of 10X is a high-quality powder mix for preparation of 10X buffer concentrate for dilution to a 1X working solution commonly used as a wash solution for western blot membranes and microtiter plate wells in ELISA assays. Tris Buffered Saline with Tween 20 (TBST) is an optimal formulation of pH stabilizers, salts and detergents designed to effectively remove excess material from the microtiter plate wells without disrupting the ELISA binding reaction. By maintaining the proper buffering environment, unbound components can be washed away without suppressing antigen-antibody binding interactions, thereby reducing nonspecific background and increasing the specific signal.
Also available without Tween 20: TBS: 1 L of 1X (sc-362185) TBS: 1 L of 10X (sc-24951) TBS: 100 ml of 10X (sc-362303) TBS: 500 ml of 10X (sc-362304) TBS: 1 L of 20X (sc-362186) TBS: 5 L of 20X (sc-362305) TBS: powder for 100 ml of 10X (sc-362188) TBS: powder for 500 ml of 10X (sc-362306) TBS: powder for 1 L of 10X (sc-362307) TBS: powder for 5 L of 10X (sc-362308)
Reconstitute powder in ddH20 and empty contents of Tween-20 vial (rinse vial thoroughly) into liquid, bringing final volume to 100 ml (10X). Filter solution through a 0.22 μm filter into a sterile flask. Makes 1 L of 1X TBST buffer.
Store the buffer solution at +4°C. Final concentration of Tween-20 (1X solution) = 0.05%. Final (1X) pH 7.4 ±0.2 (may need slight pH adjustment).
When diluted to a 1X concentration, the pH will be ~7.4.
Store at room temperature
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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Rated 5 out of
Various publications cite the use of TrisVarious publications cite the use of Tris Buffered Saline with Tween 20 to effectively remove excess material from the microtiter plate wells without disrupting the ELISA binding reaction. By maintaining the proper buffering environment, unbound components can be washed away without suppressing antigen-antibody binding interactions, thereby reducing nonspecific background and increasing the specific signal. -SCBT Publication Review
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