Tris Buffered Saline with Tween 20: 5 L of 20X A convenient prepared buffer for use in various applications

Tris Buffered Saline with Tween 20: 5 L of 20X

Tris Buffered Saline with Tween 20: 5 L of 20X is rated 5.0 out of 5 by 3.
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Synonym: 20X TBST
Application: A convenient prepared buffer for use in various applications
* Refer to Certificate of Analysis for lot specific data (including water content).
Submit a review for this product and receive 15 CruzCredits

Tris Buffered Saline with Tween 20: 5 L of 20X is a high-quality 20X concentrate for dilution to a 1X working solution commonly used as a wash solution for western blot membranes and microtiter plate wells in ELISA assays. Tris Buffered Saline with Tween 20 (TBST) is an optimal formulation of pH stabilizers, salts and detergents designed to effectively remove excess material from the microtiter plate wells without disrupting the ELISA binding reaction. By maintaining the proper buffering environment, unbound components can be washed away without suppressing antigen-antibody binding interactions, thereby reducing nonspecific background and increasing the specific signal.

Additional sizes and formats available:
Tris Buffered Saline with Tween 20: 1 L of 1X (sc-362187)
Tris Buffered Saline with Tween 20: 100 ml of 10X (sc-362309)
Tris Buffered Saline with Tween 20: 500 ml of 10X (sc-362310)
Tris Buffered Saline with Tween 20: 1 L of 10X (sc-24953)
Tris Buffered Saline with Tween 20: 1 L of 20X (sc-281695)
Tris Buffered Saline with Tween 20: powder for 100 ml of 10X (sc-362190)
Tris Buffered Saline with Tween 20: powder for 500 ml of 10X (sc-362312)
Tris Buffered Saline with Tween 20: powder for 1 L of 10X (sc-362313)
Tris Buffered Saline with Tween 20: powder for 5 L of 10X (sc-362314)

Also available without Tween 20:
Tris Buffered Saline: 1 L of 1X (sc-362185)
Tris Buffered Saline: 1 L of 10X (sc-24951)
Tris Buffered Saline: 100 ml of 10X (sc-362303)
Tris Buffered Saline: 500 ml of 10X (sc-362304)
Tris Buffered Saline: 1 L of 20X (sc-362186)
Tris Buffered Saline: 5 L of 20X (sc-362305)
Tris Buffered Saline: powder for 100 ml of 10X (sc-362188)
Tris Buffered Saline: powder for 500 ml of 10X (sc-362306)
Tris Buffered Saline: powder for 1 L of 10X (sc-362307)
Tris Buffered Saline: powder for 5 L of 10X (sc-362308)

Usage :
Dilute to a 1X solution with ddH20. Filter solution through a 0.22 μm filter into a sterile flask. Makes 100 L of 1X TBST buffer.
Store the buffer solution at +4°C. Final concentration of Tween-20 (1X solution) = 0.05%.
pH :
When diluted to a 1X concentration, the pH will be ~7.4.
Storage :
Store at 4° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Download SDS (MSDS)

Certificate of Analysis

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Tris Buffered Saline with Tween 20: 5 L of 20X  Product Citations

See how others have used Tris Buffered Saline with Tween 20: 5 L of 20X. Click on the entry to view the PubMed entry .

Citations 1 to 10 of 11 total

PMID: # 27150226  Kondaurova, EM. et al. 2016. Behavioural brain research. 310: 20-5.

PMID: # 26893688  Shi, J. et al. 2016. Oncology letters. 11: 1043-1050.

PMID: # 27009863  Bhadury, J. et al. 2016. Oncotarget.

PMID: # 26622777  Seven, D. et al. 2015. Oncology letters. 10: 1927-1931.

PMID: # 25312823  Liu, SS. et al. 2014. Biomedicine & pharmacotherapy = Biomédecine & pharmacothérapie. 68: 1031-6.

PMID: # 25270520  Minn, YK. et al. 2014. International journal of oncology. 45: 2403-10.

PMID: # 24713807  Wagner, JM. et al. 2014. PloS one. 9: e94401.

PMID: # 23224340  Shoae-Hassani, A. et al. 2013. Archivum immunologiae et therapiae experimentalis. 61: 75-83.

PMID: # 23110163  Fallica, B. et al. 2012. PloS one. 7: e48024.

PMID: # 19232100  Stockwin, LH. et al. 2009. BMC cancer. 9: 63.

Citations 1 to 10 of 11 total

What is the concentration of Tween 20 in this TBS? 0.5%? 2%? Thanks

Asked by: SMitha
Thank you for your question. Tris Buffered Saline with Tween 20: 5 L of 20X (sc-362311) is a provided as a 20X concentrate for dilution to a 1X working solution. The final concentration of Tween-20 (1X solution) = 0.05%.
Answered by: Technical Support
Date published: 2017-03-13

Does this product contain any preservatives like sodium azide, or thimerosal, or BSA or Gelatin ?

Asked by: chemicalsmg
Thank you for your question. sc-362311, Tris Buffered Saline with Tween 20: 5 L of 20X does not contain sodium azide, or thimerosal, or BSA or Gelatin. sc-362311 is sterile-filtered (0.22µM-filtered) into the container, and ambient shipping is ok. Long-term storage at ~4°C is recommended.
Answered by: Chemical Support 3
Date published: 2017-02-28
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Rated 5 out of 5 by from works very well We bought Tween 20: 5 L of 20X, 5 L, and it is very convenient to use. The data is consistant too.
Date published: 2017-04-03
Rated 5 out of 5 by from Very convenient Very convenient
Date published: 2015-10-12
Rated 5 out of 5 by from Has been working great for us Has been working great for us. No issues.
Date published: 2015-06-16
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