Tris Buffered Saline with Tween 20: 1 L of 10X A convenient prepared buffer for use in various applications

Tris Buffered Saline with Tween 20: 1 L of 10X

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Synonym: 10X TBST
Application: A convenient prepared buffer for use in various applications
* Refer to Certificate of Analysis for lot specific data (including water content).
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Tris Buffered Saline with Tween 20: 1 L of 10X is a high-quality 10X concentrate for dilution to a 1X working solution commonly used as a wash solution for western blot membranes and microtiter plate wells in ELISA assays. Tris Buffered Saline with Tween 20 (TBST) is an optimal formulation of pH stabilizers, salts and detergents designed to effectively remove excess material from the microtiter plate wells without disrupting the ELISA binding reaction. By maintaining the proper buffering environment, unbound components can be washed away without suppressing antigen-antibody binding interactions, thereby reducing nonspecific background and increasing the specific signal.

Additional sizes and formats available:
Tris Buffered Saline with Tween 20: 1 L of 1X (sc-362187)
Tris Buffered Saline with Tween 20: 100 ml of 10X (sc-362309)
Tris Buffered Saline with Tween 20: 500 ml of 10X (sc-362310)
Tris Buffered Saline with Tween 20: 1 L of 20X (sc-281695)
Tris Buffered Saline with Tween 20: 5 L of 20X (sc-362311)
Tris Buffered Saline with Tween 20: powder for 100 ml of 10X (sc-362190)
Tris Buffered Saline with Tween 20: powder for 500 ml of 10X (sc-362312)
Tris Buffered Saline with Tween 20: powder for 1 L of 10X (sc-362313)
Tris Buffered Saline with Tween 20: powder for 5 L of 10X (sc-362314)

Also available without Tween 20:
Tris Buffered Saline: 1 L of 1X (sc-362185)
Tris Buffered Saline: 1 L of 10X (sc-24951)
Tris Buffered Saline: 100 ml of 10X (sc-362303)
Tris Buffered Saline: 500 ml of 10X (sc-362304)
Tris Buffered Saline: 1 L of 20X (sc-362186)
Tris Buffered Saline: 5 L of 20X (sc-362305)
Tris Buffered Saline: powder for 100 ml of 10X (sc-362188)
Tris Buffered Saline: powder for 500 ml of 10X (sc-362306)
Tris Buffered Saline: powder for 1 L of 10X (sc-362307)
Tris Buffered Saline: powder for 5 L of 10X (sc-362308)

Usage :
Dilute to a 1X solution with ddH20. Filter solution through a 0.22 µm filter into a sterile flask. Makes 10 L of 1X TBST buffer. Store the buffer solution at +4°C. Final concentration of Tween-20 (1X solution) = 0.05%.
Formulation :
10X
Physical State :
Liquid
pH :
as a [1X], the pH will be ~7.4.
Storage :
Store at 4° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

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Certificate of Analysis

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Hello, Is it necessary to use a filter? can I just dilute the 10X solution by adding 10 ml of the conc. TBST to 900 of water?

Asked by: Oceana
Thank you for your question. Dilute to a 1X solution with ddH20. Filter solution through a 0.22 µm filter into a sterile flask. Makes 10 L of 1X TBST buffer. Store the buffer solution at +4°C. Final concentration of Tween-20 (1X solution) = 0.05%. You can find more information here: https://www.scbt.com/scbt/product/tris-buffered-saline-with-tween-20-1-l-of-10x If you have any further questions, please contact Technical Service.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-11
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