Tris Buffered Saline: 5 L of 20X A convenient prepared buffer for use in various applications

Tris Buffered Saline: 5 L of 20X

Tris Buffered Saline: 5 L of 20X is rated 5.0 out of 5 by 1.
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Synonym: 20X TBS
Application: A convenient prepared buffer for use in various applications
* Refer to Certificate of Analysis for lot specific data (including water content).
Submit a review for this product and receive 15 CruzCredits

Tris Buffered Saline: 5 L of 20X is ready to use for dilution to a 1X working solution (pH 7.4). Tris Buffered Saline (TBS) is a pH stabilizing solution used for western blot and ELISA procedures. It is an optimal formulation of salts and pH stabilizers which enable washing without disruption of antibody-antigen binding interactions.

Additional sizes and formats available:
Tris Buffered Saline: 1 L of 1X (sc-362185)
Tris Buffered Saline: 1 L of 10X (sc-24951)
Tris Buffered Saline: 100 ml of 10X (sc-362303)
Tris Buffered Saline: 500 ml of 10X (sc-362304)
Tris Buffered Saline: 1 L of 20X (sc-362186)
Tris Buffered Saline: powder for 100 ml of 10X (sc-362188)
Tris Buffered Saline: powder for 500 ml of 10X (sc-362306)
Tris Buffered Saline: powder for 1 L of 10X (sc-362307)
Tris Buffered Saline: powder for 5 L of 10X (sc-362308)

Also available with Tween 20:
Tris Buffered Saline with Tween 20:1 L of 1X (sc-362187)
Tris Buffered Saline with Tween 20:100 ml of 10X (sc-362309)
Tris Buffered Saline with Tween 20:500 ml of 10X (sc-362310)
Tris Buffered Saline with Tween 20:1 L of 10X (sc-24953)
Tris Buffered Saline with Tween 20: 1 L of 20X (sc-281695)
Tris Buffered Saline with Tween 20: 5 L of 20X (sc-362311)
Tris Buffered Saline with Tween 20: powder for 100 ml of 10X (sc-362190)
Tris Buffered Saline with Tween 20: powder for 500 ml of 10X (sc-362312)
Tris Buffered Saline with Tween 20: powder for 1 L of 10X (sc-362313)
Tris Buffered Saline with Tween 20: powder for 5 L of 10X (sc-362314)


References

1. Esen, A. et al. 1983. Anal. Biochem. 132(2): 462-467. PMID: 6625179
2. Fetterhoff, T.J. and McCarthy, R.C. 1985. Am. J. Clin. Pathol. 83(5): 565-570. PMID: 3887892
3. Nagy, B. et al. 1995. Anal. Biochem. 231(1): 40-45. PMID: 8678318
4. Evers, P. and Uylings, H.B. 1997. J. Neurosci. Methods. 72(2): 197-207. PMID: 9133585
5. Rossano, M.G. et al. 2000. J. Vet. Diagn. Invest. 12(1): 28-32. PMID: 10690772

Usage :
Prior to use, dilute to a 1X solution with ddH20. Filter solution through a 0.22 μm filter into a sterile flask. Makes 100 L of 1X TBS buffer. Store the buffer solution at +4°C. Final (1X) pH 7.4 ±0.2 (25°C), may need slight pH adjustment.
pH :
When diluted to a 1X concentration, the pH will be ~7.4.
Storage :
Store at 4° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

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Certificate of Analysis

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Tris Buffered Saline: 5 L of 20X  Product Citations

See how others have used Tris Buffered Saline: 5 L of 20X. Click on the entry to view the PubMed entry .

Citations 1 to 2 of 2 total

PMID: # 24616715  Reda, A. et al. 2014. Frontiers in endocrinology. 5: 21.

PMID: # 22708801  Kjartansdóttir, KR. et al. 2012. Systems biology in reproductive medicine. 58: 330-8.

Citations 1 to 2 of 2 total

How is the formulation? (concentration NaCl and TRIS?)

Asked by: ivan c
Please see our SDS for composition information.
Answered by: Technical Support
Date published: 2018-12-20

For how long does the 20 x concentrate remains stable at room temperature?

Asked by: SCM4
Unopened Tris Buffered Saline: 20X (TBS 20X) is stable 1 year at RT. TBS solutions that become turbid should be discarded.
Answered by: Tech Service 11
Date published: 2017-03-17
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Rated 5 out of 5 by from Various publications cite Tris Buffered Saline Various publications cite Tris Buffered Saline as a gentle wash solution (without Tween 20) for SDS-PAGE; enables washing of western blot membranes without disturbing antibody-antigen binding interactions. -SCBT Publication Review
Date published: 2015-03-17
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