Tris-Borate-EDTA buffer, 5X A concentrated solution ready to use for DNA and RNA polyacrylamide gel electrophoresis

Tris-Borate-EDTA buffer, 5X (CAS 610769-35-2)

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Application: A concentrated solution ready to use for DNA and RNA polyacrylamide gel electrophoresis
CAS Number: 610769-35-2
Molecular Weight: 475.21
Molecular Formula: C14H30BN3O14
* Refer to Certificate of Analysis for lot specific data (including water content).
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Tris-Borate-EDTA (TBE) buffer, 5X is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids. Since borate in TBE buffer is a strong inhibitor for many enzymes, TAE buffer (Tris Acetate-EDTA buffer, 10X, sc-296646) is recommended when looking at enzymatic applications for the DNA sample.
Additional forms available:
Tris-Borate-EDTA buffer, 10X (sc-296650)
Tris-Borate-EDTA Buffer, 10X Powder Pack (sc-296651)


References

1. Orbán, L., et al. 1991. Electrophoresis. 12: 233-240. PMID: 2070779
2. Wu, M. and Kusukawa, N. 1998. BioTechniques. 24: 676-678. PMID: 9564543
3. Stellwagen, N.C. 1998. Electrophoresis. 19: 1542-1547. PMID: 9719523
4. Stellwagen, N.C., et al. 2000. Anal. Biochem. 287: 167-175. PMID: 11078596
5. Ren, J., et al. 2002. Anal Sci. 18: 469-471. PMID: 11999525
6. Biyani, M., et al. 2003. Electrophoresis. 24: 628-633. PMID: 12601730
7. Chen, Z. and Burns, M.A. 2005. Electrophoresis. 26: 4718-4728. PMID: 16294296
8. Zhou, P., et al. 2005. J Chromatogr A. 1083: 173-178. PMID: 16078704

Usage :
Ready for use after dilution to working concentrations. Dilution of the TBE stock concentrates to a 1X TBE running buffer results in a buffer containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5X or 10X stocks may also be added to an acrylamide/bis-acrylamide stock solution for making the PAGE gel.
Formulation :
5X
Physical State :
Liquid
Solubility :
soluble in water
pH :
~8.3
Storage :
Store at room temperature
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
PubChem CID :
21257725
SMILES :
B(O)(O)O.C(CN(CC(=O)O)CC(=O)O)N(CC(=O)O)CC(=O)O.C(C(CO)(CO)N)O

Download SDS (MSDS)

Certificate of Analysis

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