Tris-Borate-EDTA buffer, 10X A concentrated solution ready to use for DNA and RNA polyacrylamide gel electrophoresis, "TBE buffer"

Tris-Borate-EDTA buffer, 10X

Tris-Borate-EDTA buffer, 10X is rated 5.0 out of 5 by 1.
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Application: A concentrated solution ready to use for DNA and RNA polyacrylamide gel electrophoresis, "TBE buffer"
* Refer to Certificate of Analysis for lot specific data (including water content).
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Tris-Borate-EDTA (TBE) buffer, 10X is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids. Since borate in TBE buffer is a strong inhibitor for many enzymes, TAE buffer (Tris Acetate-EDTA buffer, 10X powder, sc-296647) is recommended when looking at enzymatic applications for the DNA sample.
Additional forms available:
Tris-Borate-EDTA Buffer, 10X Powder Pack (sc-296651)
Tris-Borate-EDTA buffer, 5X (sc-296652)


References

1. Orbán, L., et al. 1991. Electrophoresis. 12: 233-240. PMID: 2070779
2. Wu, M. and Kusukawa, N. 1998. BioTechniques. 24: 676-678. PMID: 9564543
3. Stellwagen, N.C. 1998. Electrophoresis. 19: 1542-1547. PMID: 9719523
4. Stellwagen, N.C., et al. 2000. Anal. Biochem. 287: 167-175. PMID: 11078596
5. Ren, J., et al. 2002. Anal Sci. 18: 469-471. PMID: 11999525
6. Biyani, M., et al. 2003. Electrophoresis. 24: 628-633. PMID: 12601730
7. Chen, Z. and Burns, M.A. 2005. Electrophoresis. 26: 4718-4728. PMID: 16294296
8. Zhou, P., et al. 2005. J Chromatogr A. 1083: 173-178. PMID: 16078704

Usage :
Ready for use after dilution to working concentrations. Dilution of the TBE stock concentrates to a 1X TBE running buffer results in a buffer containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5X or 10X stocks may also be added to an acrylamide/bis-acrylamide stock solution for making the PAGE gel.
Formulation :
10X
Solubility :
soluble in water
pH :
~8.3
Storage :
Store at room temperature
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

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Tris-Borate-EDTA buffer, 10X  Product Citations

See how others have used Tris-Borate-EDTA buffer, 10X. Click on the entry to view the PubMed entry .

Citations 1 to 1 of 1 total

PMID: # 26519197  Kartal, K. et al. 2015. BMC Cancer. 15: 824.

Citations 1 to 1 of 1 total
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Rated 5 out of 5 by from Tris Tris-borate-EDTA buffer, 10X is a concentrated solution used for DNA and RNA polyacrylamide gel electrophoresis that is cited in various publications. -SCBT Publication Review
Date published: 2015-07-12
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