Tris-Borate-EDTA Buffer, 10X Powder Pack A ready to mix buffer reagent used for DNA and RNA polyacrylamide gel electrophoresis

Tris-Borate-EDTA Buffer, 10X Powder Pack

Tris-Borate-EDTA Buffer, 10X Powder Pack is rated 5.0 out of 5 by 1.
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Application: A ready to mix buffer reagent used for DNA and RNA polyacrylamide gel electrophoresis
* Refer to Certificate of Analysis for lot specific data (including water content).

Tris-Borate-EDTA (TBE) buffer, 10X Powder Pack is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids. Since borate in TBE buffer is a strong inhibitor for many enzymes, TAE buffer (Tris Acetate-EDTA buffer, 10X powder, sc-296647) is recommended when looking at enzymatic applications for the DNA sample.
Additional forms available:
Tris-Borate-EDTA buffer, 10X (sc-296650)
Tris-Borate-EDTA buffer, 5X (sc-296652)


References

1. Orbán, L., et al. 1991. Electrophoresis. 12: 233-240. PMID: 2070779
2. Wu, M., et al. 1998. BioTechniques. 24: 676-678. PMID: 9564543
3. Stellwagen, N.C. 1998. Electrophoresis. 19: 1542-1547. PMID: 9719523
4. Stellwagen, N.C., et al. 2000. Anal. Biochem. 287: 167-175. PMID: 11078596
5. Ren, J., et al. 2002. Anal Sci. 18: 469-471. PMID: 11999525
6. Biyani, M., et al. 2003. Electrophoresis. 24: 628-633. PMID: 12601730
7. Chen, Z., et al. 2005. Electrophoresis. 26: 4718-4728. PMID: 16294296
8. Zhou, P., et al. 2005. J Chromatogr A. 1083: 173-178. PMID: 16078704

Usage :
Ready for use following dilution to working concentrations. Each packet provides an adequate amount of material to make 1 L of 10X concentrate that can be diluted to a 1X solution containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5X or 10X stocks may also be added to an acrylamide/bis-acrylamide stock solution for making PAGE gels.
Formulation :
10X
Physical State :
Solid
Solubility :
soluble in water
pH :
~8.3
Storage :
Store at room temperature
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

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Certificate of Analysis

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What is the weight in grams in one packet of this 10X powder - sc296651.

Asked by: AlexeyK
Thank you for your question. It is approximately 171.5 g.
Answered by: Tech Service
Date published: 2019-01-28
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Rated 5 out of 5 by from Use it routinely in our lab Use it routinely in our lab, no complaints.
Date published: 2015-08-04
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