Tris Acetate-EDTA buffer, 50X For DNA agarose gel electrophoresis; also used for non-denaturing RNA agarose gel electrophoresis

Tris Acetate-EDTA buffer, 50X

Tris Acetate-EDTA buffer, 50X is rated 5.0 out of 5 by 2.
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Application: For DNA agarose gel electrophoresis; also used for non-denaturing RNA agarose gel electrophoresis
* Refer to Certificate of Analysis for lot specific data (including water content).
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Tris-Acetate-EDTA (TAE) Buffer is supplied as a 50X concentrate that can be diluted to a 1X solution containing 40 mM Tris-acetate, and 1 mM EDTA, pH ~8.3. Tris Acetate-EDTA buffer is used for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis. Double-stranded DNA tends to run faster in TAE than in other buffers but may become exhausted during extended electrophoresis. Buffer circulation or buffer replacement during extended electrophoresis can remedy the lower buffering capacity. May be used at various concentrations to study the mobility of DNA in solution. Since borate in TBE buffer (Tris-Borate-EDTA Buffer, 10X Powder Pack, sc-296651) is a strong inhibitor for many enzymes, TAE buffer is recommended when looking at enzymatic applications for the DNA sample.
Additional forms available:
Tris Acetate-EDTA buffer (sc-296645)
Tris Acetate-EDTA buffer, 10X (sc-296647)


References

1. Loening, U.E. 1967. Biochem. J. 102: 251-257. PMID: 5339944
2. Ogden, R.C., et al. 1987. Meth. Enzymol. 152: 61-87. PMID: 2443811
3. Hayes, V.M., et al. 1999. Nucleic Acids Res. 27: e29. PMID: 10497279
4. Stellwagen, E., et al. 2002. Electrophoresis. 23: 1935-1941. PMID: 12116139
5. Brody, J.R., et al. 2004. Anal. Biochem. 333: 1-13. PMID: 15351274

Usage :
Ready for use following dilution to working concentrations. 50X concentrate can be diluted to a 1X solution containing 40 mM Tris, 20 mM acetate, and 1 mM EDTA, pH ~8.3. May also be added to an acrylamide/bis-acrylamide stock solution for making PAGE gels. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.
Solubility :
Soluble in water
pH :
~8.3
Storage :
Store at room temperature
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

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Hi IS THE PRODUCT sc-281694 IS DNASE/RNASE FREE ?

Asked by: HENA
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Rated 5 out of 5 by from Good price Convenient size bottle at a good price. Please add more sizes.
Date published: 2018-10-17
Rated 5 out of 5 by from Various publications cite the use of Tris Acetate Various publications cite the use of Tris Acetate-EDTA buffer for DNA gel electrophoresis. -SCBT Publication Review
Date published: 2015-04-26
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