Tricine A buffer component for separation of low molecular weight peptides

Tricine (CAS 5704-04-1)

Tricine | CAS 5704-04-1 is rated 5.0 out of 5 by 2.
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Synonym: N-[Tris(hydroxymethyl)methyl]glycine; N-tris(Hydroxymethyl)methylglycine
Application: A buffer component for separation of low molecular weight peptides
CAS Number: 5704-04-1
Purity: ≥99%
Molecular Weight: 179.17
Molecular Formula: C6H13NO5
* Refer to Certificate of Analysis for lot specific data (including water content).
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Tricine is an organic derivative of tris and glycine used in buffer solutions. It is a zwitterionic amino acid with a useful buffering range of pH 7.4-8.8 and is one of Good's buffering agents; Good first prepared tricine to buffer chloropast reactions. A zwitterionic amino acid with a buffering range of pH 7.4-8.8. Due to a low negative charge and a high ionic strength, Tricine is very useful for electrophoretic separation of small molecular weight proteins in the range of 1 to 100 kDa. Tricine is a commonly used electrophoresis buffer and is also used in resuspension of cell pellets. It has a lower negative charge than glycine allowing it to migrate faster. In addition its high ionic strength causes more ion movement and less protein movement. This allows for low molecular weight proteins to be separated in lower percent acrylamide gels. Tricine has been documented in the separation of proteins in the range of 1 to 100 kDa by electrophoresis.[2] The tricine buffer at 25 mmol/L was found to be the most effective buffer among the ten tested for ATP assays using firefly luciferase.[3] Tricine has also been found to be an effective scavenger of hydroxyl radicals in a study of radiation-induced membrane damage. Buffer component for separation of low molecular weight peptides. A zwitterionic buffer useful in the pH range of 7.4-8.8. Has a pKa of 8.05 at 25°C


References

1. Webster, J.J., et al. 1980. Anal. Biochem. 106: 7-11. PMID: 7191217
2. Schägger, H. and von Jagow, G. 1987. Anal. Biochem. 166: 368-379. PMID: 2449095
3. Feener, E.P., et al. 1994. Biochem. J. 303: 43-50. PMID: 7945263

Physical State :
Solid
Solubility :
Soluble in water (89.6 g/l) at 20° C.
Storage :
Store at room temperature
Melting Point :
187° C
Boiling Point :
~500.92° C at 760 mmHg (Predicted)
Density :
~1.5 g/cm3 (Predicted)
Refractive Index :
n20D 1.55 (Predicted)
pK Values :
pKa: 8.1 at 25ºC
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
PubChem CID :
79784
Merck Index :
14: 9651
MDL Number :
MFCD00004277
EC Number :
227-193-6
Beilstein Registry :
1937804
SMILES :
C(C(=O)O)NC(CO)(CO)CO

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Can this product be used as a buffer for SDS-PAGE systems?

Asked by: hawkeye11
This chemical, Tricine (CAS 5704-04-1), is typically the buffer of choice in SDS-PAGE systems when separating proteins in the range of 1 to 100 kDa.
Answered by: Chemical Support 1
Date published: 2017-03-22
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Rated 5 out of 5 by from Used in WB Used in WB, worked well
Date published: 2015-03-30
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