Towbin, without SDS is a western blot transfer buffer for use with nitrocellulose and PVDF transfer membranes. Towbin buffer is a Tris-glycine buffer with a pH of 8.3.This product is recommended for use when transferring proteins that are highly soluble and have proven to elute from gels easily. If protein transfer is inefficient, a suitable alternative may be: Towbin, with SDS, 10X (sc-24954).
1. Towbin, H., et al. 1979. Proc. Natl. Acad. Sci. U.S.A. 76: 4350-4354. PMID: 388439
2. Towbin, H., et al. 1992. Biotechnology. 24: 145-149. PMID: 1422008
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