Thiazolyl Blue A membrane-permeable dye reduced by mitochondrial enzymes

Thiazolyl Blue (CAS 298-93-1)

Thiazolyl Blue | CAS 298-93-1 is rated 5.0 out of 5 by 3.
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Synonym: MTT; Thiazolyl Blue Tetrazolium Bromide
Application: A membrane-permeable dye reduced by mitochondrial enzymes
CAS Number: 298-93-1
Purity: ≥98%
Molecular Weight: 414.32
Molecular Formula: C18H16BrN5S
* Refer to Certificate of Analysis for lot specific data (including water content).
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Thiazolyl Blue is a membrane-permeable yellow dye that is reduced by mitochondrial reductases in living cells to form the dark blue product, MTT Formazan (sc-215399). Spectrophotometric measurement of MTT Formazan at 540 or 570 nm allows quantitation of cell viability. Thiazolyl Blue is used as a direct indicator of cytotoxicity (such as for screening cancer drugs) and apoptosis. Thiazolyl Blue is also an electron acceptor used for studying NADP-dependent dehydrogenases.

Physical State :
Solid
Solubility :
Soluble in water (20 mg/ml), ethanol (20 mg/ml), 2-methoxyethanol (20 mg/ml), buffered salt solutions (5 mg/ml), culture media (5 mg/ml), and PBS (5 mg/ml).
Storage :
Store at 4° C
Melting Point :
195° C (dec.)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
RTECS :
XF8060000
PubChem CID :
64965
MDL Number :
MFCD00011964
EC Number :
206-069-5
Beilstein Registry :
3825277
SMILES :
CC1=C(SC(=N1)[N+]2=NC(=NN2C3=CC=CC=C3)C4=CC=CC=C4)C.[Br-]

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Thiazolyl Blue  Product Citations

See how others have used Thiazolyl Blue. Click on the entry to view the PubMed entry .

Citations 1 to 10 of 11 total

PMID: # 27404580  2016. Eur J Pharm Sci. 92: 183-93.

PMID: # 27137748  Aldahoun, MA. et al. 2016. Artif Cells Nanomed Biotechnol. 1-11.

PMID: # 26748999  Minaei, A. et al. 2016. Mol. Biol. Rep. 43: 99-105.

PMID: # 26214469  Wang, XW. et al. 2015. Genet. Mol. Res. 14: 7883-93.

PMID: # 26108872  Zahedifard, M. et al. 2015. Sci Rep. 5: 11544.

PMID: # 25040994  Sabzichi, M. et al. 2014. Asian Pac. J. Cancer Prev. 15: 5311-6.

PMID: # 23294370  Xu, M. et al. 2013. Hepatol. Res. 43: 890-905.

PMID: # 24036512  Salim, LZ. et al. 2013. Molecules. 18: 11219-40.

PMID: # 22609744  Xu, M. et al. 2012. Toxicol. Lett. 212: 113-25.

PMID: # 22261727  Rani, MP. et al. 2012. J. Sci. Food Agric. 92: 1948-55.

Citations 1 to 10 of 11 total

Can this product be used on human cell tissue culture or animal cell tissue culture?

Asked by: SCchem16
Thank you for your question. Yes, this product is cell culture grade and can be used with any cell type. Please contact Technical Service if you have further questions concerning this product.
Answered by: Tech Service 9
Date published: 2017-01-05

What is the solubility of this product?

Asked by: hawkeye11
Thank you for your question. This chemical is soluble in water and ethanol (20 mg/ml), and PBS or other buffered solutions at 5 mg/ml. Please contact Technical Service if you have further questions concerning this product.
Answered by: Tech Service 9
Date published: 2016-12-20
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Rated 5 out of 5 by from MTT Very usufull for cytotoxicity assays. I used it in Neurochemistry International 59(2):297-308 · June 2011
Date published: 2017-01-26
Rated 5 out of 5 by from Buenos resultados y económico Empleamos la técnica de reducción de MTT de manera rutinaria en el laboratorio para determinar muerte celular por ser sencilla, reproducible y económica. Si bien solíamos usar el reactivo de otra marca, pasamos a adquirir el de Santa Cruz por su precio. Obtuvimos exactamente los mismos resultados.
Date published: 2017-01-26
Rated 5 out of 5 by from Thiazolyl Blue is a yellow dye that is reduced Thiazolyl Blue is a yellow dye that is reduced by mitochondrial reductases in living cells to form MTT Formazan, which is then used as an indicator of cytotoxicity and apoptosis. It is also used for the study of NADP-dependent dehydrogenases, as it is an electron acceptor. -SCBT Publication Review
Date published: 2015-01-16
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