TBS Blotto B Blocking buffer and antibody diluent for both primary and secondary antibody incubations to reduce non-specific binding and background in Western blotting applications; preferred over BSA

TBS Blotto B: sc-2335

TBS Blotto B is rated 4.5 out of 5 by 2.
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Datasheets
    • TBS Blotto B, specifically recommended for use with phospho-specific antibodies, is supplied as a lyophilized powder in a single-use bottle; final reconstituted volume of 500 mL
    • Blocking buffer and antibody diluent for both primary and secondary antibody incubations to reduce non-specific binding and background in Western blotting applications; preferred over BSA
    • Immunoanalytical Grade Blotto contains 1% milk, 1% blocking reagent, 0.05% Tween-20; Tween-20 is included in a separate vial in liquid form
    • makes a 1x solution when reconstituted to the final volume of 500 ml in deionized H2O
    • To inhibit serine/threonine phosphatase activity, add 50 mM Sodium Flouride (NaF) (sc-24988) to Blotto. And/or Sodium Orthovanadate (Na3O4V) (sc-3540) to inhibit protein tyrosine phosphatase activity. Alternatively, add Phosphatase Inhibitor Cocktail A (sc-45044) and Phosphatase Inhibitor Cocktail B (sc-45045)
    • Recommended to reduce overall background, and minimize non-specific signals commonly associated with enhanced chemiluminescent (ECL) protein detection methods using HRP conjugated primary and secondary antibodies, or mouse IgG binding proteins
    • If using direct conjugated primary antibodies or for near-infrared (NIR) detection methods, we strongly recommend our alternative product, UltraCruz® Blocking Reagent (sc-516214)
    • If not blotting with phospho-specific antibodies, we recommend our alternative product, TBS Blotto A (sc-2333) which contains a unique formulation of 5% milk, 0.05% Tween-20
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Are you able to confirm what the pack size (weight in g) of SC-2335 TBS blotto B is?

Asked by: Jz28sail
Thank you for your question. The powder is approximately 16.2 to 16.4 grams, and the liquid is approximately 1.05 mL. Please note, sc-2335 is not designed to be used in part. In order to ensure the correct formulation, you will need to reconstitute the entire product as directed. If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Tech Service 8
Date published: 2017-02-06
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Rated 5 out of 5 by from Perfect! Better then i before used that only milk or others!
Date published: 2018-02-03
Rated 4 out of 5 by from No comment No comment
Date published: 2015-03-31
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