TBS Blotto A is supplied as a lyophilized powder in a single-use bottle; final reconstituted volume of 500 mL
Blocking buffer and antibody diluent for both primary and secondary antibody incubations to reduce non-specific binding and background in Western blotting applications; preferred over BSA
contains 5% milk, 0.05% Tween-20; Tween-20 is included in a separate vial in liquid form
makes a 1x solution when reconstituted to the final volume of 500 ml in deionized H2O
TBS Blotto A is the preferred choice of blocking buffer and antibody diluent over BSA or alternative blocking buffers; strongly recommended to be used as an antibody diluent for both primary and secondary antibody incubations to reduce non-specific binding and background
Recommended to reduce overall background, and minimize non-specific signals commonly associated with enhanced chemiluminescent (ECL) protein detection methods using HRP conjugated primary and secondary antibodies, or mouse IgG binding proteins
If using direct conjugated primary antibodies or for near-infrared (NIR) detection methods, we strongly recommend our alternative product, UltraCruz® Blocking Reagent (sc-516214)
For phospho-specific antibodies, we recommend our alternative product, TBS Blotto B (sc-2335) which contains a unique formulation of 1% milk, 1% blocking reagent, 0.05% Tween-20
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Santa Cruz Biotechnology offers a range of Blocking Reagents for use in Western blotting and other research applications. It is important to select the appropriate blocking buffer for your research applications in order to reduce overall background, and minimize non-specific signals commonly associated with enhanced chemiluminescent (ECL) protein detection methods using Horseradish Peroxidase (HRP) conjugated primary and secondary antibodies, or mouse IgG binding proteins.TBS Blotto A is recommended to be used for blocking, and as an antibody diluent for both primary and secondary antibody incubations.
To place an order using RMB or to ship to mainland China, please visit www.scbio.cn
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