Tankyrase-1/2 Antibody (E-10)recommended for detection of Tankyrase-1 and Tankyrase-2 of mouse, rat and human origin by WB, IP, IF and ELISA

Tankyrase-1/2 Antibody (E-10): sc-365897

Tankyrase-1/2 Antibody (E-10) is rated 5.0 out of 5 by 8.
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Datasheets
    • Tankyrase-1/2 Antibody (E-10) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 745-1094 of Tankyrase -1 of human origin
    • recommended for detection of Tankyrase-1 and Tankyrase-2 of mouse, rat and human origin by WB, IP, IF and ELISA
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Tankyrase-1/2 (E-10): sc-365897.
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I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with Tankyrase-1/2 (E-10): sc-365897?

Asked by: TinTin
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 7
Date published: 2017-01-13

I would like to use Tankyrase-1/2 (E-10) for IF, what dilution should I use for the primary antibody?

Asked by: TinTin
Thank you for your question. We recommend a starting dilution of 1:50 for IF and IHC applications. Please contact Technical Service if you have any further questions or if you would like more information regarding the recommended protocol.
Answered by: Technical Support 7
Date published: 2017-01-13
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Rated 5 out of 5 by from 试用了一次,效果很好 我上一周使用了Tankyrase-1/2 抗体 sc-365897,跑了abcam的tnks2抗体特异性不大,这次用了santa感觉结果还可以。
Date published: 2019-04-16
Rated 5 out of 5 by from Works for WB/IF/PLA Bought this antibody (Tank-1/2) to measure protein-protein interactions (amongst other things) in 293T/HeLa lysates. Worked great! WB 1:2500 and IF/PLA 1:500.
Date published: 2017-08-15
Rated 5 out of 5 by from Great antibody for TNKS1/2 This antibody is specific and is suitable for detection of TNKS2 in HEK293 cells. I used the antibody at 1:500, and the signal was pretty good
Date published: 2017-07-23
Rated 5 out of 5 by from an excellent antibody very good antibody for WB,recognises both TNKS1 and TNKS2
Date published: 2017-06-28
Rated 5 out of 5 by from Beautiful staining Brilliant signal seen with methanol fixed cells in IF.
Date published: 2017-01-18
Rated 5 out of 5 by from Comparison between E-10 and H-340 It is several times more sensitive than the discontinued rabbit polyclonal (H-340). It picks up TNKS as a single band in several extracts tested.
Date published: 2016-12-09
Rated 5 out of 5 by from Produced superb immunofluorescence nuclear Produced superb immunofluorescence nuclear and cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-01-21
Rated 5 out of 5 by from Nice Western Blot data of Tankyrase Nice Western Blot data of Tankyrase-1/2 expression in Jurkat whole cell lysate. -SCBT QC
Date published: 2013-07-29
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