Streptavidin A tetrameric protein used to visualize biotin conjugated molecules in ELISA

Streptavidin (CAS 9013-20-1)

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Application: A tetrameric protein used to visualize biotin conjugated molecules in ELISA
CAS Number: 9013-20-1
Purity: ≥95%
Molecular Weight: ~55kD
* Refer to Certificate of Analysis for lot specific data (including water content).
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Streptavidin is a 55kDa tetrameric protein secreted by Streptomyces avidinii which binds firmly to biotin (sc-204706). Streptavidin is wildly used in molecular biology through its unique high affinity for the biotin. The dissociation constant (Kd) of the biotin-streptavidin complex is about ~1015 M. The strong affinity of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. The streptavidin/biotin system has one of the biggest free energies of association of yet observed for noncovalent binding of a protein and small ligand in aqueous solution. The complexes are also extremely stable over a wide range of temperature and pH. The Streptavidin preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogeneity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket as compared to native streptavidin. Streptavidin has a molecular weight of 55kDa.


References

1. Meyer, D.L., et al. 2001. Protein Sci. 10: 491-503. PMID: 11344318
2. Rusakiewicz, S., et al. 2009. Cancer Immunol. Immunother. 58: 1459-1470. PMID: 19415272
3. Yokota, S., 2010. Methods Mol. Biol. 657: 109-119. PMID: 20602210
4. Stone, J.D., et al. 2011. J. Immunol. 187: 6281-6290. PMID: 22102724
5. Du, L., et al. 2012. Luminescence. 27: 28-33. PMID: 21695770

Usage :
Specific Activity: Greater than 15.7 U/mg, 1 unit binds 1µg biotin. Proteolytic Activity: Less than 10-3 U/mg protein (Azocoll, 25°C, 24 h, pH 8.0). Streptavidin although stable at 4°C for 3 weeks, should be stored desiccated below -18°C. For longer storage in dissolved form add 1mM EDTA and/or 0.02 % NaN3 or pass the solution through a sterile filter. Please prevent freeze-thaw cycles.
Formulation :
Lyophilized (1mg/ml) in 50mM NaCl, pH 9.0
Appearance :
Lyophilized powder
Physical State :
Solid
Derived From :
Streptomyces avidinii
Solubility :
Gives a clear solution at 5mg/ml in 0.1M NaCl.
Storage :
Desiccate at -20° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

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Certificate of Analysis

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this product dye conjugated or not?

Asked by: mdbusra
Thank you for the question. Streptavidin (CAS 9013-20-1) is a 55kDa tetrameric protein secreted by Streptomyces avidinii which binds firmly to biotin (sc-204706). It us useful to visualize biotin conjugated molecules in various applications such as e.g. ELISA or IHC(P). This product sc-363872 itself is not conjugated.
Answered by: Technical Support Europe
Date published: 2018-11-22

can streptavidin (CAS 9013-20-1) be used to detect biotin conjugated peptide with the help of hrp conjugated secondary antibody in elisa and western blot?

Asked by: zilleanam
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