Sodium phosphate tribasic dodecahydrate Often used in the formulation of various buffers

Sodium phosphate tribasic dodecahydrate (CAS 10101-89-0)

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Synonym: Trisodium phosphate dodecahydrate
Application: Often used in the formulation of various buffers
CAS Number: 10101-89-0
Purity: >97%
Molecular Weight: 380.12
Molecular Formula: Na3PO4 12H2O
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* Refer to Certificate of Analysis for lot specific data (including water content).
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Sodium phosphate tribasic dodecahydrate is also known as trisodium phospate or TSP. It is highly soluble in water and widely used as a cleaning agent. The pH of a 1% solution is typically around 12, making this reagent sufficiently alkaline for saponification of grease and oils. Often used in the formulation of various buffers; complementary buffer components may include: Sodium phosphate monobasic (sc-215883; sc-251042), Sodium phosphate dibasic (sc-203277; sc-236917) or Potassium Phosphate, Dibasic (sc-203210). Trisodium phosphate is used in the isolation of mycobacteria, including culturing of tubercle bacilli. Has been used as a protein precipitant in certain applications and in co-precipitation and fractionization of nucleotides. May be used for the preservation of biologically active soil samples. Because of the alkaline pH, TSP disrupts cytoplasmic and outer membranes of Salmonella enterica cells making it useful in preventing the spread of bacteria.
Anhydrous form also available: Trisodium phosphate (sc-251041).


References

1. Armstrong, A.R. 1948. Can Med Assoc J. 59: 73. PMID: 18864250
2. Martin, M.E., et al. 1968. Appl Microbiol. 16: 506-508. PMID: 4967759
3. Chan, P.S. and Lin, M.C. 1974. Meth. Enzymol. 38: 38-41. PMID: 4375771
4. MacGregor, C.H. 1978. Meth. Enzymol. 53: 347-355. PMID: 362126
5. Sampathkumar, B., et al. 2003. Appl. Environ. Microbiol. 69: 122-129. PMID: 12513986
6. Rosell, M., et al. 2006. J Chromatogr A. 1132: 28-38. PMID: 16904119

Appearance :
Crystalline powder
Physical State :
Solid
Solubility :
Soluble in water (258 mg/ml at 20° C). Insoluble in alcohol.
Storage :
Store at room temperature
Melting Point :
~75° C (dec.)
Boiling Point :
158° C at 760 mmHg (Predicted)
Density :
1.62 g/cm3 at 25° C (lit.)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
1
RTECS :
TC9575000
PubChem CID :
61473
Merck Index :
14: 8662
MDL Number :
MFCD00149198
EC Number :
231-509-8
SMILES :
O.O.O.O.O.O.O.O.O.O.O.O.[O-]P(=O)([O-])[O-].[Na+].[Na+].[Na+]

Download SDS (MSDS)

Certificate of Analysis

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