Sodium Citrate Tribasic Dihydrate (Molecular Biology Grade) For the preparation of total ribosomal RNA from E. coli

Sodium Citrate Tribasic Dihydrate (Molecular Biology Grade) (CAS 6132-04-3)

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Synonym: Citric acid trisodium salt dihydrate; Trisodium citrate dihydrate
Application: For the preparation of total ribosomal RNA from E. coli
CAS Number: 6132-04-3
Purity: ≥99.5%
Molecular Weight: 294.10
Molecular Formula: C6H5O72H2O•3Na
* Refer to Certificate of Analysis for lot specific data (including water content).
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Sodium Citrate Tribasic Dihydrate (Molecular Biology Grade) is a source of Citric acid, a key metabolic intermediate. Citrate is the starting point of the tricarboxylic acid cycle. Its concentration also coordinates several other metabolic pathways. Citric acid can form complexes with various cations, particularly with iron and calcium. In animals, citric acid improves the utilization of nutritional calcium.

Usage :
Preparation Instructions:
One gram of sodium citrate dissolves in 1.3 ml of water at 25 °C and in 0.6 ml of boiling water. Poorly soluble in alcohol.

pH: 7.5 - 9.0 (0.1 M in H2O, 25°C)

Storage/Stability:
Citrate buffers (pH 3-5) exhibit excellent stability at room temperature. Dilute solutions of citric acid (non-sterile) may ferment if left at room temperature. Non-sterile solutions should be stable for months stored at 2-8 °C.
Appearance :
Powder
Physical State :
Solid
Solubility :
Soluble in water (29.4 g/L at 20 °C). Insoluble in alcohol.
Storage :
Store at room temperature
Melting Point :
>300° C (lit.)
Boiling Point :
309.6° C at 760 mmHg
Density :
1.86 g/cm3
Refractive Index :
n20D ~1.58 (Predicted)
pK Values :
pKa: pK1: 3.14, pK2: 4.76, pK3: 6.40
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
1
PubChem CID :
71474
Merck Index :
14: 8602
MDL Number :
MFCD00150031
EC Number :
200-675-3
Beilstein Registry :
6104939
SMILES :
C(C(=O)[O-])C(CC(=O)[O-])(C(=O)[O-])O.O.O.[Na+].[Na+].[Na+]

Download SDS (MSDS)

Certificate of Analysis

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