Sodium Chloride Used in biochemistry and molecular biology applications; a component of PBS and SSC buffers

Sodium Chloride (CAS 7647-14-5)

Sodium Chloride | CAS 7647-14-5 is rated 5.0 out of 5 by 2.
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Synonym: Table salt; Halite
Application: Used in biochemistry and molecular biology applications; a component of PBS and SSC buffers
CAS Number: 7647-14-5
Purity: ≥98%
Molecular Weight: 58.44
Molecular Formula: NaCl
* Refer to Certificate of Analysis for lot specific data (including water content).
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Sodium Chloride (NaCl) is widely used in biochemistry and molecular biology applications. It is found in nature, in all body tissue, and is considered an essential nutrient. Used in a wide variety of biochemical applications including creating density gradients, regulating biological functions and as a diluent to increase ionic strength in buffers or culture media. NaCl is a component of phosphate buffered saline (PBS) (sc-24946, sc-24947, sc-286634, sc-296027) and SSC buffer (sc-296419). It has also been used for precipitation of DNA from SDS-containing samples and in the removal of small nucleic acid fragments from plasmid DNA preparations. The use of sodium chloride for isolation of DNA from mammalian cells has been described. Recent studies provide evidence that a high amount of NaCl reduces production of nitric oxide.


References

1. Hathaway, G.M., et al. 1979. Meth. Enzymol. 60: 495-511. PMID: 459914
2. Iglewski, B.H., et al. 1979. Meth. Enzymol. 60: 780-793. PMID: 111001
3. Leckband, D.E., et al. 1993. Biochemistry. 32: 1127-1140. PMID: 8424941
4. Discipio, R.G., et al. 1998. Acta Crystallogr. D Biol. Crystallogr. 54: 643-646. PMID: 9761862
5. Smith, B.S., et al. 1998. Acta Crystallogr. D Biol. Crystallogr. 54: 697-699. PMID: 9761879
6. Curtis, R.A., et al. 2002. Biophys. Chem. 98: 249-265. PMID: 12128178 7. Osanai, T. et al. 2002. Blood Purif. 20(5): 466-468. PMID: 12207094

Usage :
• If intended for use in cell culture applications, sterilization of the solution may be necessary. Pre-diluted buffer can be dispensed into aliquots and sterilized by autoclaving.
Physical State :
Solid
Solubility :
Soluble in water (358 mg/ml at 20° C), alcohol (very slightly), glycerol (100 mg/ml), and ammonia. Insoluble in hydrochloric acid.
Storage :
Store at room temperature
Melting Point :
801° C (lit.)
Boiling Point :
1413° C
Density :
2.17 g/cm3 at 20° C
Refractive Index :
n20D 1.54
Ki Data :
Astrosclerin-3: Ki= 460 µM (Astrosclera willeyana); CA I: Ki= 6 mM (human); CA II: Ki= 200 mM (human)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
1
RTECS :
VZ4725000
PubChem CID :
5234
Merck Index :
14: 8599
MDL Number :
MFCD00003477
EC Number :
231-598-3
Beilstein Registry :
3534976
SMILES :
[Na+].[Cl-]

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Certificate of Analysis

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Sodium Chloride  Product Citations

See how others have used Sodium Chloride. Click on the entry to view the PubMed entry .

Citations 1 to 2 of 2 total

PMID: # 24962405  Ahn, JH. et al. 2014. J. Vet. Sci. 15: 335-42.

PMID: # 10751433  Fujimura, M. et al. 2000. J Neurosci. 20(8): 2817-2824.

Citations 1 to 2 of 2 total
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Rated 5 out of 5 by from My go-to NaCl On a per-price basis, SCBT's NaCl is comparable or better priced than competing vendors, especially when one asks for a quote. I have never had a problem with any solution made from this reagent.
Date published: 2017-02-15
Rated 5 out of 5 by from Various publications cite the multiple uses Various publications cite the multiple uses of Sodium Chloride in a wide variety of applications. A few examples of its many uses include: creating density gradients, regulating biological functions, and as a component of PBS (sc-24946, sc-24947, sc-286634, sc-296027) and SSC buffer (sc-296419). -SCBT Publication Review
Date published: 2015-03-14
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