SMS1 Antibody (G-8)recommended for detection of SMS1 of mouse, rat and human origin by WB, IP, IF and ELISA

SMS1 Antibody (G-8): sc-133135

SMS1 Antibody (G-8) is rated 3.0 out of 5 by 2.
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Datasheets
    • SMS1 Antibody (G-8) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 21-150 mapping at the N-terminus of SMS1 of human origin
    • recommended for detection of SMS1 of mouse, rat and human origin by WB, IP, IF and ELISA
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of SMS1 (G-8): sc-133135.
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For Western Blot, is it recommended to use denatured or non-denatured conditions with SMS1 (G-8): sc-133135 monoclonal antibody?

Asked by: AbPolly
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-02-27
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Rated 1 out of 5 by from No Signal in WB Used at a 1:500 dilution on 35ug of HeLa lysate and did not get a signal after 30min of exposure
Date published: 2016-12-06
Rated 5 out of 5 by from Good Western blot data of SMS1 expression in non Good Western blot data of SMS1 expression in non-transfected and human SMS1 transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-05-23
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