Smoothened Agonist, HCl An Hh pathway agonist that interacts with the heptahelical domain of Smo

Smoothened Agonist, HCl (CAS 364590-63-6)

Smoothened Agonist, HCl | CAS 364590-63-6 is rated 5.0 out of 5 by 1.
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Synonym: SAG HCl; N-Methyl-N'-(3-pyridinylbenzyl)-N'-(3-chlorobenzo[b]thiophene-2-carbonyl)-1,4-diaminocyclohexane, SAG1.3
Application: A Hh pathway agonist that interacts with the heptahelical domain of Smo
CAS Number: 364590-63-6
Purity: ≥95%
Molecular Weight: 562.98
Molecular Formula: C28H28ClN3OS•2HCl
* Refer to Certificate of Analysis for lot specific data (including water content).
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Smoothened Agonist, HCl (SAG), is a highly potent activator of Smo (smoothened). This chlorobenzothiophene compound is a hedgehog pathway agonist that interacts with the heptahelical domain of Smo. SAG is shown to act as an activator at low concentrations, but can inhibit Smo coupling at very high concentrations. The activating effects of SAG will counteract Cyclopamine (sc-200929) or Cyclopamine-KAAD (sc-221448) inhibition of Smo.


References

1. Frank-Kamenetsky, M., et al. 2002. J. Biol. 1: 10. PMID: 12437772

2. Chen, J.K., et al. 2002. Proc. Natl. Acad. Sci. U.S.A. 99: 14071-14076. PMID: 12391318

3. Chen, W., et al. 2004. Science. 306: 2257-2260. PMID: 15618519

4. Meloni, A.R., et al. 2006. Mol. Cell. Biol. 26: 7550-7560. PMID: 16908539

5. Corcoran, R.B., et al.2006. Proc. Natl. Acad. Sci. U.S.A. 103: 8408-8413. PMID: 16707575

Usage :
Stable for up to 1 year after receipt when stored at -20°C. Stock solutions should be aliquoted and stored at -20°C for up to 1 month.
Physical State :
Solid
Solubility :
Soluble in water (25 mg/ml), and DMSO (10 mg/ml).
Storage :
Store at 4° C
IC50 :
Hedgehog pathway activation: EC5050 = 3 nM (NIH 3T3-derived Shh-LIGHT2 cells)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
PubChem CID :
71433770
SMILES :
CNC1CCC(CC1)N(CC2=CC=CC(=C2)C3=CC=NC=C3)C(=O)C4=C(C5=CC=CC=C5S4)Cl.O.Cl.Cl

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Certificate of Analysis

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Smoothened Agonist, HCl  Product Citations

See how others have used Smoothened Agonist, HCl. Click on the entry to view the PubMed entry .

Citations 1 to 10 of 22 total

PMID: # 26123494  Shaheen, R. et al. 2015. Human molecular genetics. 24: 5211-8.

PMID: # 25044881  Bertacchi, M. et al. 2015. Dev Neurobiol. 75: 66-79.

PMID: # 26286140  Lee, SJ. et al. 2015. Molecular cancer. 14: 159.

PMID: # 26370082  Fu, X. et al. 2015. J. Biol. Chem. 290: 26445-56.

PMID: # 25260753  Melboucy-Belkhir, S. et al. 2014. American journal of physiology. Lung cellular and molecular physiology. 307: L838-47.

PMID: # 24726283  Zhulyn, O. et al. 2014. Dev. Cell. 29: 241-9.

PMID: # 24647919  Jiang, X. et al. 2014. J. Cell. Physiol. 229: 1016-27.

PMID: # 23069989  Bertacchi, M. et al. 2013. Cell. Mol. Life Sci. 70: 1095-111.

PMID: # 23650387  Coles, GL. et al. 2013. Proceedings of the National Academy of Sciences of the United States of America. 110: E1898-905.

PMID: # 23602386  Xiong, Y. et al. 2013. Dev. Cell. 25: 169-81.

Citations 1 to 10 of 22 total

What is the appearance of the compound?

Asked by: two2igm05
Thank you for your question. Smoothened Agonist, HCl, sc-202814, is a light yellow solid.
Answered by: Chemical Support 4
Date published: 2017-03-29

I was wondering after dissolve the material, how would you store it and for how long would the product be active?

Asked by: 13punMA
Thank you for asking about our product Smoothened Agonist, HCl (CAS# 364590-63-6) We recommend that the you use the solution within the same day. If you do need to store their solution they should aliquot it out and store at -20ºC in the dark. It can be stored frozen for up to 6 months.
Answered by: Chemical Support 8
Date published: 2017-03-10

Is this product a mix of cis/trans (about 1- N and 4-methylamino in cyclochexyl)?

Asked by: 13punMA
Thank you for asking about our product Smoothened Agonist, HCl (CAS# 364590-63-6). This product is not a mixture of cis/trans. It is only a trans isomer.
Answered by: Chemical Support 8
Date published: 2017-03-10
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Rated 5 out of 5 by from This worked fine for us This worked fine for us.
Date published: 2015-03-31
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