Sec8 Antibody (B-11)recommended for detection of Sec8 of mouse, rat and human origin by WB, IP, IF and ELISA

Sec8 Antibody (B-11): sc-514215

Sec8 Antibody (B-11) is rated 4.8 out of 5 by 4.
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Datasheets
    • Sec8 Antibody (B-11) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 675-974 mapping at the C-terminus of Sec8 of human origin
    • recommended for detection of Sec8 of mouse, rat and human origin by WB, IP, IF and ELISA
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Sec8 (B-11): sc-514215.
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What blocking solution should I use with Sec8 (B-11): sc-514215 monoclonal antibody for Western blot?

Asked by: TinTin
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-02-28
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Rated 4 out of 5 by from Good for IF and IHC This Sec8 antibody shows strong specific signal in IF and IHC stainings in primary rat motor neuron.
Date published: 2017-11-06
Rated 5 out of 5 by from an excellent antibody for both WB and IF This antibody recognizes a clear and strong band of predicted M.W. on the western blot (1:500 dilution, 40 ug U251 lysate).
Date published: 2017-08-28
Rated 5 out of 5 by from Good for Westerns Successful detection of Sec8 in MDCK whole cell lysates - incubated @ 1:500 O/N at 5˚C.
Date published: 2017-07-19
Rated 5 out of 5 by from Satisfactory Western blot data of Sec8 expression Satisfactory Western blot data of Sec8 expression in non-transfected 293T, human Sec8 transfected 293T HeLa and Jurkat whole cell lysates and HeLa nuclear extract . -SCBT QC
Date published: 2014-06-07
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