SAP 30BP Antibody (G-8)recommended for detection of SAP 30BP of mouse, rat and human origin by WB, IP, IF and ELISA

SAP 30BP Antibody (G-8): sc-514278

SAP 30BP Antibody (G-8) is rated 3.0 out of 5 by 2.
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Datasheets
    • SAP 30BP Antibody (G-8) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 77-230 mapping within an internal region of SAP 30BP of human origin
    • recommended for detection of SAP 30BP of mouse, rat and human origin by WB, IP, IF and ELISA
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of SAP 30BP (G-8): sc-514278.
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What blocking solution should I use with SAP 30BP (G-8): sc-514278 monoclonal antibody for Western blot?

Asked by: TinTin
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 1 out of 5 by from Weak antibody Very weak antibody. Band visible at 1:100 dilution only after 1-hour exposition on film. Tested in a HCT116 whole cell lysate.
Date published: 2018-09-03
Rated 5 out of 5 by from Great Western blot data of SAP 30BP expression Great Western blot data of SAP 30BP expression in MOLT-4, MCF7 and HeLa whole cell lysates. -SCBT QC
Date published: 2013-11-14
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