Running Buffer, 10X Tris-Glycine WB Running Buffer, pH 8.3; for sodium dodecyl sulfate – polyacrylamide gel electrophoresis (SDS-PAGE)

Running Buffer, 10X

Running Buffer, 10X is rated 5.0 out of 5 by 1.
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Application: Tris-Glycine WB Running Buffer, pH 8.3; for sodium dodecyl sulfate – polyacrylamide gel electrophoresis (SDS-PAGE)
* Refer to Certificate of Analysis for lot specific data (including water content).
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Running Buffer, 10X is a Tris-Glycine buffer used for sodium dodecyl sulfate – polyacrylamide gel electrophoresis (SDS-PAGE) of proteins. It is used as both the anode and cathode buffer. Running Buffer, 10X is supplied as 1L of 10X concentrate that can be diluted to a 1X solution containing 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3. Researchers may check and adjust the final pH using a sensitive pH meter.


References

1. Laemmli, U.K. 1970. Nature. 227: 680-685. PMID: 5432063
2. Roder, K., et al. 2001. Biotechnol. Appl. Biochem. 33: 209-214. PMID: 11389675

Usage :
Dilute to a 1X solution with ddH20.
Physical State :
Liquid
pH :
8.3
Storage :
Store at 4° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

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Running Buffer, 10X  Product Citations

See how others have used Running Buffer, 10X. Click on the entry to view the PubMed entry .

Citations 1 to 1 of 1 total

PMID: # 22708801  Kjartansdóttir, KR. et al. 2012. Systems biology in reproductive medicine. 58: 330-8.

Citations 1 to 1 of 1 total
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Rated 5 out of 5 by from Numerous publications cite the use of Running Numerous publications cite the use of Running Buffer, 10X (Tris-Glycine, pH 8.3), in their Western Blot applications. -SCBT Publication Review
Date published: 2015-03-17
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