RNA Sample Loading Buffer A solution which promotes protein denaturation for use in SDS-PAGE and is ethidium bromide-free

RNA Sample Loading Buffer

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Application: A solution which promotes protein denaturation for use in SDS-PAGE and is ethidium bromide-free
* Refer to Certificate of Analysis for lot specific data (including water content).
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Supplied as 5 vials of 250 μL.

RNA Sample Loading Buffer is formulated for use in RNA sample preparation for denaturing formaldehyde-agarose gel electrophoresis. Formulated for use in Northern blots. This product is suitable for use in formaldehyde-agarose gel electrophoresis of RNA. It has been tested for RNase activity and none was detected. Free of Ethidium Bromide to optimize transfer efficiency.

Composition of RNA Sample Loading Buffer:
62.5% (v/v) deionized formamide
1.14 M formaldehyde
1.25X MOPS-EDTA-sodium acetate buffer
200 μg/ml bromophenol blue
200 μg/ml xylene cyanol FF


References

1. Rosen, K.M., et al. 1990. Biotechniques. 8: 398-403. PMID: 1692716

Usage :
Add 1 volume sample to 2-5 volumes of sample loading buffer and mix well. Immediately prior to loading, heat sample to 65 °C for 10 minutes, then chill on ice.
Physical State :
Liquid
Storage :
Store at -20° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
MDL Number :
MFCD00213041

Download SDS (MSDS)

Certificate of Analysis

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