RIPA Lysis Buffer System A high quality, ready to use mammalian cell and tissue lysis buffer with protease inhibitors included

RIPA Lysis Buffer System

RIPA Lysis Buffer System is rated 4.8 out of 5 by 29.
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Application: RIPA Lysis Buffer is a mammalian cell lysis buffer containing protease inhibitors. Supplied as 4 separate reagent vials that are combined immediately prior to use.
* Refer to Certificate of Analysis for lot specific data (including water content).
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RIPA (Radioimmunoprecipitation) Lysis Buffer is used to lyse cells and tissue, for radio immunoprecipitation assay (RIPA). RIPA lysis buffer has stronger denaturing capabilities than NP-40 (sc-281108) or Triton X-100 (sc-29112) and is particularly useful for disruption of nuclear membranes in the preparation of nuclear extracts.

This high quality product includes protease inhibitors, making it ready for use in mammalian cell and tissue lysis.

sc-24948, 50 mL - Components supplied in four vials:
VIAL 1: 50 mL 1X lysis buffer (pH 7.4 ±0.1)
VIAL 2: 500 μL (200mM) PMSF in DMSO
VIAL 3: 500 μL protease inhibitor cocktail in DMSO
VIAL 4: 500 μL (100mM) sodium orthovanadate in water

sc-24948A, 500 mL - Components supplied in four vials:
VIAL 1: 500 mL 1X lysis buffer
VIAL 2: 5 ml (200mM) PMSF in DMSO
VIAL 3: 5 ml protease inhibitor cocktail in DMSO
VIAL 4: 5 ml (100mM) sodium orthovanadate in water


References

1. Poirier, F., et al. 1982. Int. J. Cancer. 29: 69-76. PMID: 6277805
2. DeSeau, V., et al. 1987. J. Cell. Biochem. 35: 113-128. PMID: 2448318
3. Bolen, J.B., et al. 1987. Oncogene Res. 1: 149-168. PMID: 2453014
4. Ngoka, L.C. 2008. Proteome Sci. 6: 30. PMID: 18950484

Usage :
• Immediately prior to lysing cells, combine 10 μl PMSF solution, 10 μl sodium orthovanadate solution and 10-20 μl protease inhibitor cocktail solution per ml of 1X RIPA Lysis buffer to prepare complete RIPA.
• Use 3 ml complete RIPA per gram of tissue
- OR 1 ml complete RIPA per 2.0x107 cells in suspension
- OR 0.6 ml complete RIPA per subconfluent monolayer on a 100 mm plate.
Physical State :
Liquid
Storage :
Store at -20° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Download SDS (MSDS)

Certificate of Analysis

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RIPA Lysis Buffer System  Product Citations

See how others have used RIPA Lysis Buffer System. Click on the entry to view the PubMed entry .

Citations 1 to 10 of 1699 total

PMID: # 28157110  Chen, W. et al. 2017. Restor. Neurol. Neurosci. 35: 217-224.

PMID: # 28315692  Ma, J. et al. 2017. Toxicol. Appl. Pharmacol. 323: 16-25.

PMID: # 28551696  Wang, LJ. et al. 2017. Med. Sci. Monit. 23: 2590-2598.

PMID: # 28553222  Nair, RR. et al. 2017. Front Synaptic Neurosci. 9: 8.

PMID: # 28974241  Yan, Y. et al. 2017. Virol. J. 14: 190.

PMID: # 28389511  Iida, Y. et al. 2017. Clin. Cancer Res. 23: 4831-4842.

PMID: # 27864336  Yang, S. et al. 2017. Reproduction. 153: 211-220.

PMID: # 28134774  Baba, Y. et al. 2017. Int J Mol Sci. 18:

PMID: # 28775096  Singh, B. et al. 2017. Am. J. Physiol. Lung Cell Mol. Physiol. ajplung.00184.2017.

PMID: # 28052723  Shaik, S. et al. 2017. Stem Cells Dev. 26: 608-616.

Citations 1 to 10 of 1699 total

Can you tell us the components of the protease inhibitor cocktail? We are performing downstream applications that are sensitive to serine protease inhibition and want to confirm that a serine protease inhibitor is not present in the cocktail.

Asked by: medchem
Thank you for your question. The complete protease inhibitor components are considered proprietary. However, we can confirm that there is a serine protease inhibitor present.
Answered by: Technical Support
Date published: 2019-03-15

Can I use this buffer for solid phase sandwich ELISA

Asked by: Wajid
Thank you for your question. We haven’t used this for solid phase sandwich ELISA. It does contain denaturing detergents so it may not be ideal for this application, depending on your protocol.
Answered by: Technical Support
Date published: 2019-02-10

What kinds of detergent is in RIPA (sc24948)? Is RIPA a good lysate buffer of heart total protein extraction? Thank you.

Asked by: SHENGNAN WU
Thank you for your question. The RIPA Lysis Buffer System uses the following detergents: 1% Nonidet P-40, 0.5% sodium deoxycholate and 0.1% SDS. This is a popular choice for protein extraction of tissues, including heart tissue.
Answered by: Technical Support
Date published: 2019-02-10

Please, which is the composition of this lysis buffer? (https://www.scbt.com/scbt/product/ripa-lysis-buffer-system Thank you.

Asked by: Ca36
Thank you for your question. Here is the composition of the lysis buffer component (1x Lysis Buffer): 1x TBS, 1% Nonidet P-40, 0.5% sodium deoxycholate, 0.1% SDS, 0.004% sodium azide.
Answered by: Technical Support
Date published: 2019-02-10

What is the composition of the RIPA buffer?

Asked by: Steffi W
Thank you for your question. Here is the composition of the lysis buffer component (1x Lysis Buffer): 1x TBS, 1% Nonidet P-40, 0.5% sodium deoxycholate, 0.1% SDS, 0.004% sodium azide.
Answered by: Technical Support
Date published: 2019-02-07

Since the protease inhibitor in a vial, Do we need to make aliquot of it and store at -20°C?

Asked by: Sara1
The kit is designed to make as needed since long term storage of protease inhibitors in aqueous solution is not recommended.
Answered by: Technical Support
Date published: 2019-01-26

If I need to take for instance 2 ml of RIPA buffer and supplement it with protease inhibitor, Do we need to freeze and thaw each time the RIPA buffer? I mean should we store the stock (without protease inhibitor) at -20°C?

Asked by: Sara1
Thank you for your question. For 50mL lysis buffer, we recommend to make aliquots of 5 vials (10mL) each. Do one freeze/thaw cycle, discard unused. For remaining vials, use fresh. Add inhibitors (after allowing to thaw) to lysis buffer right before use, discard unused (do not re-store) as the inhibitors degrade over time.
Answered by: Technical Support
Date published: 2019-01-26

Does this kit interfere with pierce bca protein assay ? Since the "blank" containing just the reagents in this kit and water becomes light violet with and Absorbance around 0.300

Asked by: lsnucia
Thank you for your question. This kit is compatible with the BCA assay which we routinely use in-house. Please contact our Technical Service Department for further support.
Answered by: Technical Support
Date published: 2019-01-26
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Rated 5 out of 5 by from Works great! Works great for lysing mammalian cell for western blots. No slimy/viscous pellets in the lysate.
Date published: 2018-10-30
Rated 5 out of 5 by from Great cost performance Although this RIPA lysis buffer kit contains protease inhibitor cocktail and phosphatase inhibitors, cost is inexpensive! Of course, it works well!
Date published: 2018-02-22
Rated 4 out of 5 by from how to make it working 1x ripa buffer as per its mentioned that for 1 ml we have to add particular content..do we have to reduce the amount of other content from the 1 ml and then add or in 1 ml we can add 10 ul of each component???
Date published: 2017-12-17
Rated 5 out of 5 by from Very good for whole cell lysis Purchased this item few weeks ago and used it on our HEK293 cells. I really like how they have separate vials for PMSF, orthovanadate and protease inhibitors included in the package at sufficient amounts. The isolation worked great!
Date published: 2017-11-09
Rated 5 out of 5 by from RIPA lysis buffer This RIPA lysis buffer system contains buffer, protease inhibitor cocktail, phosphatase inhibitors. Easy to use.
Date published: 2017-04-28
Rated 5 out of 5 by from Outstanding product I have been using this buffer system for last 15 years. It always worked for extraction process for different cell lines. Highly recommended.
Date published: 2017-02-06
Rated 5 out of 5 by from great In our experiment will often use this product, easy to operate, can get good results, the effect is very good!
Date published: 2017-02-06
Rated 5 out of 5 by from very good This system works good at our lab! I always buy this buffer.
Date published: 2017-01-23
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