RIPA Lysis Buffer System A high quality, ready to use mammalian cell and tissue lysis buffer with protease inhibitors included

RIPA Lysis Buffer System

RIPA Lysis Buffer System is rated 4.8 out of 5 by 29.
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Application: RIPA Lysis Buffer is a mammalian cell lysis buffer containing protease inhibitors. Supplied as 4 separate reagent vials that are combined immediately prior to use.
* Refer to Certificate of Analysis for lot specific data (including water content).
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RIPA (Radioimmunoprecipitation) Lysis Buffer is used to lyse cells and tissue, for radio immunoprecipitation assay (RIPA). RIPA lysis buffer has stronger denaturing capabilities than NP-40 (sc-281108) or Triton X-100 (sc-29112) and is particularly useful for disruption of nuclear membranes in the preparation of nuclear extracts.

This high quality product includes protease inhibitors, making it ready for use in mammalian cell and tissue lysis.

sc-24948, 50 mL - Components supplied in four vials:
VIAL 1: 50 mL 1X lysis buffer (pH 7.4 ±0.1)
VIAL 2: 500 μL (200mM) PMSF in DMSO
VIAL 3: 500 μL protease inhibitor cocktail in DMSO
VIAL 4: 500 μL (100mM) sodium orthovanadate in water

sc-24948A, 500 mL - Components supplied in four vials:
VIAL 1: 500 mL 1X lysis buffer
VIAL 2: 5 ml (200mM) PMSF in DMSO
VIAL 3: 5 ml protease inhibitor cocktail in DMSO
VIAL 4: 5 ml (100mM) sodium orthovanadate in water


References

1. Poirier, F., et al. 1982. Int. J. Cancer. 29: 69-76. PMID: 6277805
2. DeSeau, V., et al. 1987. J. Cell. Biochem. 35: 113-128. PMID: 2448318
3. Bolen, J.B., et al. 1987. Oncogene Res. 1: 149-168. PMID: 2453014
4. Ngoka, L.C. 2008. Proteome Sci. 6: 30. PMID: 18950484

Usage :
• Immediately prior to lysing cells, combine 10 μl PMSF solution, 10 μl sodium orthovanadate solution and 10-20 μl protease inhibitor cocktail solution per ml of 1X RIPA Lysis buffer to prepare complete RIPA.
• Use 3 ml complete RIPA per gram of tissue
- OR 1 ml complete RIPA per 2.0x107 cells in suspension
- OR 0.6 ml complete RIPA per subconfluent monolayer on a 100 mm plate.
Physical State :
Liquid
Storage :
Store at -20° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Download SDS (MSDS)

Certificate of Analysis

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RIPA Lysis Buffer System  Product Citations

See how others have used RIPA Lysis Buffer System. Click on the entry to view the PubMed entry .

Citations 1 to 10 of 1699 total

PMID: # 28157110  Chen, W. et al. 2017. Restor. Neurol. Neurosci. 35: 217-224.

PMID: # 28315692  Ma, J. et al. 2017. Toxicol. Appl. Pharmacol. 323: 16-25.

PMID: # 28551696  Wang, LJ. et al. 2017. Med. Sci. Monit. 23: 2590-2598.

PMID: # 28553222  Nair, RR. et al. 2017. Front Synaptic Neurosci. 9: 8.

PMID: # 28974241  Yan, Y. et al. 2017. Virol. J. 14: 190.

PMID: # 28389511  Iida, Y. et al. 2017. Clin. Cancer Res. 23: 4831-4842.

PMID: # 27864336  Yang, S. et al. 2017. Reproduction. 153: 211-220.

PMID: # 28134774  Baba, Y. et al. 2017. Int J Mol Sci. 18:

PMID: # 28775096  Singh, B. et al. 2017. Am. J. Physiol. Lung Cell Mol. Physiol. ajplung.00184.2017.

PMID: # 28052723  Shaik, S. et al. 2017. Stem Cells Dev. 26: 608-616.

Citations 1 to 10 of 1699 total

Salve, potrei avere informazioni sulla composizione esatta del vostro buffer? Grazie

Asked by: Donatella88
Thank you for your question. The components of the RIPA Lysis Buffer System are listed on the product page and SDS in section 3: COMPOSITION/INFORMATION ON INGREDIENTS
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

Is the RIPA lysis buffer system suitable for label free mass spec. analysis of the whole proteome?

Asked by: BT99
Thank you for your question. Please contact our technical service with the specifications of your experiment so we can help you figure out whether this product would be suitable for your application.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

I would like to know which are the detergents in the RIPA buffer. I'm struggling with a downstream application that doesn't like detergents and that information would help me a lot to troubleshoot my problem. Thank you, Riccardo

Asked by: RiccardoA
Thank you for your question. RIPA Lysis Buffer System: sc-24948 consists of Nonidet P-40, sodium deoxycholate and sodium dodecyl sulfate detergents.
Answered by: Technical Support
Date published: 2018-12-05

Dear Santa Cruz, does this product contain protease inhibitor and if I use this inhibitor is it enough to cell lysis or I should use an complete inhibitor product?

Asked by: Simin
Thank you very much for the question. Our RIPA Lysis Buffer sc-24948 is a widely established high quality, ready to use mammalian cell and tissue lysis buffer with protease inhibitors included. We suggest to use this product according to our protocol https://www.scbt.com/scbt/de/resources/protocols/western-immuno-blotting You might also want to check the corresponding datasheet for additional details.
Answered by: Technical Support Europe
Date published: 2018-02-21

After making, is it ok to keep complete RIPA in -20 for further use or is it to be made freshly before every lysis ?

Asked by: HHEN
Thank you for your question. Our RIPA Lysis Buffer System is designed to prepare for use as needed because long term storage of protease inhibitors in aqueous solution is not recommended.
Answered by: Technical Support
Date published: 2018-01-04

Does this buffer contain NaF?

Asked by: Gucci
Thank you for your question. There is NaF in the protease inhibitor cocktail supplied with our RIPA Lysis Buffer System: sc-24948.
Answered by: Technical Support
Date published: 2017-09-23

I want to use Transglutaminase Assay Kit (sigma CS1070) to detect enzyme activity of cell lines. Can I use RIPA buffer from santa cruz to lysis cells?

Asked by: youyoumama
Thank you for your question. We would recommend that you contact the other supplier of the specific enzyme assay kit to inquire about the compatibility of the components in their kit with standard cell lysis buffers such as our RIPA Lysis Buffer System: sc-24948.
Answered by: Technical Support
Date published: 2017-08-30

Will it interfere with Bradford Assay?

Asked by: Elvira
Thank you for your question. No, the RIPA Lysis Buffer System does not contain substances that interfere with a Bradford assay. We do recommend including a positive control protein with a known concentration as a reference. Depending on the method and timing of your absorbance measurements, it is expected that you may still observe a difference of 10-20% in concentration.
Answered by: Technical Support
Date published: 2017-08-16
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Rated 5 out of 5 by from Works great! Works great for lysing mammalian cell for western blots. No slimy/viscous pellets in the lysate.
Date published: 2018-10-30
Rated 5 out of 5 by from Great cost performance Although this RIPA lysis buffer kit contains protease inhibitor cocktail and phosphatase inhibitors, cost is inexpensive! Of course, it works well!
Date published: 2018-02-22
Rated 4 out of 5 by from how to make it working 1x ripa buffer as per its mentioned that for 1 ml we have to add particular content..do we have to reduce the amount of other content from the 1 ml and then add or in 1 ml we can add 10 ul of each component???
Date published: 2017-12-17
Rated 5 out of 5 by from Very good for whole cell lysis Purchased this item few weeks ago and used it on our HEK293 cells. I really like how they have separate vials for PMSF, orthovanadate and protease inhibitors included in the package at sufficient amounts. The isolation worked great!
Date published: 2017-11-09
Rated 5 out of 5 by from RIPA lysis buffer This RIPA lysis buffer system contains buffer, protease inhibitor cocktail, phosphatase inhibitors. Easy to use.
Date published: 2017-04-28
Rated 5 out of 5 by from Outstanding product I have been using this buffer system for last 15 years. It always worked for extraction process for different cell lines. Highly recommended.
Date published: 2017-02-06
Rated 5 out of 5 by from great In our experiment will often use this product, easy to operate, can get good results, the effect is very good!
Date published: 2017-02-06
Rated 5 out of 5 by from very good This system works good at our lab! I always buy this buffer.
Date published: 2017-01-23
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