RIPA Lysis Buffer System (w/out PMSF) A high quality, ready to use mammalian cell and tissue lysis buffer with protease inhibitors included

RIPA Lysis Buffer System (w/out PMSF)

RIPA Lysis Buffer System (w/out PMSF) is rated 5.0 out of 5 by 1.
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Application: A high quality, ready to use mammalian cell and tissue lysis buffer with protease inhibitors included
* Refer to Certificate of Analysis for lot specific data (including water content).
Submit a review for this product and receive 15 CruzCredits

RIPA (Radioimmunoprecipitation) Lysis Buffer (w/out PMSF) is used to lyse cells and tissue, for radio immunoprecipitation assay (RIPA). RIPA lysis buffer has stronger denaturing capabilities than NP-40 (sc-281108) or Triton X-100 (sc-29112) and is particularly useful for disruption of nuclear membranes in the preparation of nuclear extracts.

This high quality product includes protease inhibitors, making it ready for use in mammalian cell and tissue lysis.

sc-364162, 50 mL - Components supplied in three vials:
VIAL 1: 50 mL 1X lysis buffer
VIAL 2: 500 μL protease inhibitor cocktail in DMSO
VIAL 3: 500 μL (100mM) sodium orthovanadate in water

sc-364162A, 500 mL - Components supplied in three vials:
VIAL 1: 500 mL 1X lysis buffer
VIAL 2: 5 ml protease inhibitor cocktail in DMSO
VIAL 3: 5 ml (100mM) sodium orthovanadate in water


References

1. Poirier, F. et al. 1982. Int. J. Cancer. 29(1): 69-76. PMID: 6277805 2. DeSeau, V. et al. 1987. J. Cell. Biochem. 35(2): 113-128. PMID: 2448318 3. Bolen, J.B. et al. 1987. Oncogene Res. 1(2): 149-168. PMID: 2453014 4. Ngoka, L.C. 2008. Proteome Sci. 6

Usage :
• Immediately prior to lysing cells, combine 10 μl sodium orthovanadate solution and 10-20 μl protease inhibitor cocktail solution per ml of 1X RIPA Lysis buffer to prepare complete RIPA.

• Use 3 ml complete RIPA per gram of tissue

- OR 1 ml complete RIPA per 2.0x107 cells in suspension

- OR 0.6 ml complete RIPA per subconfluent monolayer on a 100 mm plate.
Storage :
Store at -20° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Download SDS (MSDS)

Certificate of Analysis

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RIPA Lysis Buffer System (w/out PMSF)  Product Citations

See how others have used RIPA Lysis Buffer System (w/out PMSF). Click on the entry to view the PubMed entry .

Citations 1 to 10 of 11 total

PMID: # 26810190  Tong, WW. et al. 2016. Tumour Biol.

PMID: # 26342052  Shao, Y. et al. 2015. J Toxicol Sci. 40: 817-28.

PMID: # 25041469  Shi, SH. et al. 2015. Am. J. Reprod. Immunol. 73: 301-12.

PMID: # 25646597  Zhao, C. et al. 2015. International journal of oncology. 46: 1643-50.

PMID: # 25821818  Pająk, B. et al. 2015. BioMed research international. 2015: 746092.

PMID: # 24199849  Duan, X. et al. 2014. Clin Radiol. 69: 186-93.

PMID: # 25669938  Zhang, L. et al. 2014. Nature genetics. 46: 726-30.

PMID: # 23867821  Dufour, M. et al. 2013. Biochem. Biophys. Res. Commun. 438: 32-7.

PMID: # 19711346  Zhu, Z. et al. 2010. Int. J. Cancer. 126: 2067-78.

PMID: # 19931970  Liu, GJ. et al. 2010. Ultrasound Med Biol. 36: 78-85.

Citations 1 to 10 of 11 total
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Rated 5 out of 5 by from Various publications cite the use of RIPA Various publications cite the use of RIPA Lysis Buffer System to lyse cells and tissue, for radio immunoprecipitation assay (RIPA). RIPA lysis buffer has stronger denaturing capabilities than NP-40 (sc-281108) or Triton X-100 (sc-29112) and is particularly useful for disruption of nuclear membranes in the preparation of nuclear extracts. -SCBT Publication Review
Date published: 2015-01-07
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