RIPA Lysis Buffer System High quality, ready to use lysis buffer with protease inhibitors included. Recommended for use in mammalian cell and tissue lysis, particularly useful in the preparation of nuclear extracts.

RIPA Lysis Buffer System

RIPA Lysis Buffer System is rated 4.8125 out of 5 by 16.
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| See 845 Citations
Application: A high quality, ready to use mammalian cell and tissue lysis buffer with protease inhibitors included
* Refer to Certificate of Analysis for lot specific data (including water content).
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Ordering Information

PRODUCT NAME CATALOG # UNIT PRICE QTY FAVORITES
RIPA Lysis Buffer System sc-24948 50 ml $29.00
RIPA Lysis Buffer System sc-24948A 500 ml $169.00

RIPA (Radioimmunoprecipitation) Lysis Buffer is used to lyse cells and tissue, for radio immunoprecipitation assay (RIPA). RIPA lysis buffer has stronger denaturing capabilities than NP-40 (sc-281108) or Triton X-100 (sc-29112) and is particularly useful for disruption of nuclear membranes in the preparation of nuclear extracts.

This high quality product includes protease inhibitors, making it ready for use in mammalian cell and tissue lysis.

sc-24948, 50 mL - Components supplied in four vials:
VIAL 1: 50 mL 1X lysis buffer (pH 7.4 ±0.1)
VIAL 2: 500 μL (200mM) PMSF in DMSO
VIAL 3: 500 μL protease inhibitor cocktail in DMSO
VIAL 4: 500 μL (100mM) sodium orthovanadate in water

sc-24948A, 500 mL - Components supplied in four vials:
VIAL 1: 500 mL 1X lysis buffer
VIAL 2: 5 ml (200mM) PMSF in DMSO
VIAL 3: 5 ml protease inhibitor cocktail in DMSO
VIAL 4: 5 ml (100mM) sodium orthovanadate in water


References

1. Poirier, F., et al. 1982. Int. J. Cancer. 29: 69-76. PMID: 6277805
2. DeSeau, V., et al. 1987. J. Cell. Biochem. 35: 113-128. PMID: 2448318
3. Bolen, J.B., et al. 1987. Oncogene Res. 1: 149-168. PMID: 2453014
4. Ngoka, L.C. 2008. Proteome Sci. 6: 30. PMID: 18950484

Usage :
• Immediately prior to lysing cells, combine 10 μl PMSF solution, 10 μl sodium orthovanadate solution and 10-20 μl protease inhibitor cocktail solution per ml of 1X RIPA Lysis buffer to prepare complete RIPA.
• Use 3 ml complete RIPA per gram of tissue
- OR 1 ml complete RIPA per 2.0x107 cells in suspension
- OR 0.6 ml complete RIPA per subconfluent monolayer on a 100 mm plate.
Physical State :
Liquid
Storage :
Store at -20° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Transport :
UN 2927, Class 6.1 (8), Packing group II

Download SDS (MSDS)

Certificate of Analysis

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RIPA Lysis Buffer System Product Citations

See how others have used RIPA Lysis Buffer System. Click on the entry to view the PubMed entry .

Citations 1 to 10 of 845 total

PMID: # 27013874
Spadavecchia, J. et al. 2016. Targeted polyethylene glycol gold nanoparticles for the treatment of pancreatic cancer: from synthesis to proof-of-concept in vitro studies. Int J Nanomedicine. 11: 791-822.

PMID: # 27047354
Poon, K. et al. 2016. Relationship of the Chemokine, CXCL12, to Effects of Dietary Fat on Feeding-Related Behaviors and Hypothalamic Neuropeptide Systems. Front Behav Neurosci. 10: 51.

PMID: # 26979530
Kunz, P. et al. 2016. Elevated ratio of MMP2/MMP9 activity is associated with poor response to chemotherapy in osteosarcoma. BMC Cancer. 16: 223.

PMID: # 27089328
Ding, Y. et al. 2016. Induction of ROS Overload by Alantolactone Prompts Oxidative DNA Damage and Apoptosis in Colorectal Cancer Cells. Int J Mol Sci. 17:

PMID: # 26646099
Troche, C. et al. 2016. Zinc transporter Slc39a14 regulates inflammatory signaling associated with hypertrophic adiposity. Am. J. Physiol. Endocrinol. Metab.. 310: E258-68.

PMID: # 26897375
Nagasawa, A. et al. 2016. t-Flavanone Improves the Male Pattern of Hair Loss by Enhancing Hair-Anchoring Strength: A Randomized, Double-Blind, Placebo-Controlled Study. Dermatol Ther (Heidelb). 6: 59-68.

PMID: # 26160454
Fauzi, I. et al. 2016. In Vitro Differentiation of Embryonic Stem Cells into Hematopoietic Lineage: Towards Erythroid Progenitor's Production. Methods Mol. Biol.. 1341: 217-34.

PMID: # 26697751
Fujita, Y. et al. 2016. Differentiated embryo chondrocyte 1 (DEC1) is a novel negative regulator of hepatic fibroblast growth factor 21 (FGF21) in aging mice. Biochem. Biophys. Res. Commun.. 469: 477-82.

PMID: # 27235551
Han, J. et al. 2016. mTOR remains unchanged in diet-resistant (DR) rats despite impaired LKB1/AMPK cascade in adipose tissue. Biochem. Biophys. Res. Commun..

PMID: # 26727223
Tian, R. et al. 2016. VEGF/VEGFR2 Signaling Regulates Germ Cell Proliferation in vitro and Promotes Mouse Testicular Regeneration in vivo. Cells Tissues Organs (Print). 201: 1-13.

Citations 1 to 10 of 845 total
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Rated 5 out of 5 by from After some time (a few years) not working After some time (a few years) not working with Protein isolation systems, I was glad to find a straightforward product that could save me time at the bench when collecting my samples. I believe the instructions are easy and my protein yield was as expected.
Date published: 2015-10-01
Rated 5 out of 5 by from we have been using it in our experiments we have been using it in our experiments as usual so it seems to be working just fine
Date published: 2015-04-16
Rated 5 out of 5 by from Used routinely in lab for WB and IP applications Used routinely in lab for WB and IP applications. No problems.
Date published: 2015-06-08
Rated 5 out of 5 by from Bought it for cell culture and WB and it Bought it for cell culture and WB and it has good quality.
Date published: 2015-07-28
Rated 5 out of 5 by from We use it routinely in our lab We use it routinely in our lab, no complaints.
Date published: 2015-04-09
Rated 5 out of 5 by from gets 5 stars gets 5 stars- works well, we buy it always
Date published: 2015-04-07
Rated 5 out of 5 by from Use routinely for WB and will order more Use routinely for WB and will order more.
Date published: 2015-09-09
Rated 5 out of 5 by from We buy this often and no issues with it We buy this often and no issues with it.
Date published: 2015-08-26
  • 2016-09-29T11:37CST
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