RIPA (Radioimmunoprecipitation) Lysis Buffer is used to lyse cells and tissue, for radio immunoprecipitation assay (RIPA). RIPA lysis buffer has stronger denaturing capabilities than NP-40 (sc-281108) or Triton X-100 (sc-29112) and is particularly useful for disruption of nuclear membranes in the preparation of nuclear extracts.
This high quality product includes protease inhibitors, making it ready for use in mammalian cell and tissue lysis.
sc-24948, 50 mL - Components supplied in four vials:
VIAL 1: 50 mL 1X lysis buffer (pH 7.4 ±0.1)
VIAL 2: 500 μL (200mM) PMSF in DMSO
VIAL 3: 500 μL protease inhibitor cocktail in DMSO
VIAL 4: 500 μL (100mM) sodium orthovanadate in water
sc-24948A, 500 mL - Components supplied in four vials:
VIAL 1: 500 mL 1X lysis buffer
VIAL 2: 5 ml (200mM) PMSF in DMSO
VIAL 3: 5 ml protease inhibitor cocktail in DMSO
VIAL 4: 5 ml (100mM) sodium orthovanadate in water
1. Poirier, F., et al. 1982. Int. J. Cancer. 29: 69-76. PMID: 6277805
2. DeSeau, V., et al. 1987. J. Cell. Biochem. 35: 113-128. PMID: 2448318
3. Bolen, J.B., et al. 1987. Oncogene Res. 1: 149-168. PMID: 2453014
4. Ngoka, L.C. 2008. Proteome Sci. 6: 30. PMID: 18950484
See how others have used RIPA Lysis Buffer System. Click on the entry to view the PubMed entry .
PMID: # 27013874
Spadavecchia, J. et al. 2016. Targeted polyethylene glycol gold nanoparticles for the treatment of pancreatic cancer: from synthesis to proof-of-concept in vitro studies. Int J Nanomedicine. 11: 791-822.
PMID: # 27047354
Poon, K. et al. 2016. Relationship of the Chemokine, CXCL12, to Effects of Dietary Fat on Feeding-Related Behaviors and Hypothalamic Neuropeptide Systems. Front Behav Neurosci. 10: 51.
PMID: # 26979530
Kunz, P. et al. 2016. Elevated ratio of MMP2/MMP9 activity is associated with poor response to chemotherapy in osteosarcoma. BMC Cancer. 16: 223.
PMID: # 27089328
Ding, Y. et al. 2016. Induction of ROS Overload by Alantolactone Prompts Oxidative DNA Damage and Apoptosis in Colorectal Cancer Cells. Int J Mol Sci. 17:
PMID: # 26646099
Troche, C. et al. 2016. Zinc transporter Slc39a14 regulates inflammatory signaling associated with hypertrophic adiposity. Am. J. Physiol. Endocrinol. Metab.. 310: E258-68.
PMID: # 26897375
Nagasawa, A. et al. 2016. t-Flavanone Improves the Male Pattern of Hair Loss by Enhancing Hair-Anchoring Strength: A Randomized, Double-Blind, Placebo-Controlled Study. Dermatol Ther (Heidelb). 6: 59-68.
PMID: # 26160454
Fauzi, I. et al. 2016. In Vitro Differentiation of Embryonic Stem Cells into Hematopoietic Lineage: Towards Erythroid Progenitor's Production. Methods Mol. Biol.. 1341: 217-34.
PMID: # 26697751
Fujita, Y. et al. 2016. Differentiated embryo chondrocyte 1 (DEC1) is a novel negative regulator of hepatic fibroblast growth factor 21 (FGF21) in aging mice. Biochem. Biophys. Res. Commun.. 469: 477-82.
PMID: # 27235551
Han, J. et al. 2016. mTOR remains unchanged in diet-resistant (DR) rats despite impaired LKB1/AMPK cascade in adipose tissue. Biochem. Biophys. Res. Commun..
PMID: # 26727223
Tian, R. et al. 2016. VEGF/VEGFR2 Signaling Regulates Germ Cell Proliferation in vitro and Promotes Mouse Testicular Regeneration in vivo. Cells Tissues Organs (Print). 201: 1-13.
Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2016 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.