RIPA Lysis Buffer System High quality, ready to use lysis buffer with protease inhibitors included. Recommended for use in mammalian cell and tissue lysis, particularly useful in the preparation of nuclear extracts.

RIPA Lysis Buffer System

RIPA Lysis Buffer System is rated 4.8 out of 5 by 24.
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| See 905 Citations
Application: A high quality, ready to use mammalian cell and tissue lysis buffer with protease inhibitors included
* Refer to Certificate of Analysis for lot specific data (including water content).
Submit a review for this product and receive 15 CruzCredits

Ordering Information

PRODUCT NAME CATALOG # UNIT PRICE QTY FAVORITES
RIPA Lysis Buffer System sc-24948 50 ml $29.00
RIPA Lysis Buffer System sc-24948A 500 ml $169.00

RIPA (Radioimmunoprecipitation) Lysis Buffer is used to lyse cells and tissue, for radio immunoprecipitation assay (RIPA). RIPA lysis buffer has stronger denaturing capabilities than NP-40 (sc-281108) or Triton X-100 (sc-29112) and is particularly useful for disruption of nuclear membranes in the preparation of nuclear extracts.

This high quality product includes protease inhibitors, making it ready for use in mammalian cell and tissue lysis.

sc-24948, 50 mL - Components supplied in four vials:
VIAL 1: 50 mL 1X lysis buffer (pH 7.4 ±0.1)
VIAL 2: 500 μL (200mM) PMSF in DMSO
VIAL 3: 500 μL protease inhibitor cocktail in DMSO
VIAL 4: 500 μL (100mM) sodium orthovanadate in water

sc-24948A, 500 mL - Components supplied in four vials:
VIAL 1: 500 mL 1X lysis buffer
VIAL 2: 5 ml (200mM) PMSF in DMSO
VIAL 3: 5 ml protease inhibitor cocktail in DMSO
VIAL 4: 5 ml (100mM) sodium orthovanadate in water


References

1. Poirier, F., et al. 1982. Int. J. Cancer. 29: 69-76. PMID: 6277805
2. DeSeau, V., et al. 1987. J. Cell. Biochem. 35: 113-128. PMID: 2448318
3. Bolen, J.B., et al. 1987. Oncogene Res. 1: 149-168. PMID: 2453014
4. Ngoka, L.C. 2008. Proteome Sci. 6: 30. PMID: 18950484

Usage :
• Immediately prior to lysing cells, combine 10 μl PMSF solution, 10 μl sodium orthovanadate solution and 10-20 μl protease inhibitor cocktail solution per ml of 1X RIPA Lysis buffer to prepare complete RIPA.
• Use 3 ml complete RIPA per gram of tissue
- OR 1 ml complete RIPA per 2.0x107 cells in suspension
- OR 0.6 ml complete RIPA per subconfluent monolayer on a 100 mm plate.
Physical State :
Liquid
Storage :
Store at -20° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Transport :
UN 2927, Class 6.1 (8), Packing group II

Download SDS (MSDS)

Certificate of Analysis

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RIPA Lysis Buffer System Product Citations

See how others have used RIPA Lysis Buffer System. Click on the entry to view the PubMed entry .

Citations 1 to 10 of 905 total

PMID: # 28099448
Fukuoka, CY. et al. 2017. The Effects of Low-Power Laser Irradiation on Inflammation and Apoptosis in Submandibular Glands of Diabetes-Induced Rats. PLoS ONE. 12: e0169443.

PMID: # 27013874
Spadavecchia, J. et al. 2016. Targeted polyethylene glycol gold nanoparticles for the treatment of pancreatic cancer: from synthesis to proof-of-concept in vitro studies. Int J Nanomedicine. 11: 791-822.

PMID: # 27047354
Poon, K. et al. 2016. Relationship of the Chemokine, CXCL12, to Effects of Dietary Fat on Feeding-Related Behaviors and Hypothalamic Neuropeptide Systems. Front Behav Neurosci. 10: 51.

PMID: # 26979530
Kunz, P. et al. 2016. Elevated ratio of MMP2/MMP9 activity is associated with poor response to chemotherapy in osteosarcoma. BMC Cancer. 16: 223.

PMID: # 27089328
Ding, Y. et al. 2016. Induction of ROS Overload by Alantolactone Prompts Oxidative DNA Damage and Apoptosis in Colorectal Cancer Cells. Int J Mol Sci. 17:

PMID: # 27484093
Ma, W. et al. 2016. The clinical significance of forkhead box protein A1 and its role in colorectal cancer. Mol Med Rep. 14: 2625-31.

PMID: # 26646099
Troche, C. et al. 2016. Zinc transporter Slc39a14 regulates inflammatory signaling associated with hypertrophic adiposity. Am. J. Physiol. Endocrinol. Metab.. 310: E258-68.

PMID: # 25552382
Treskatsch, S. et al. 2016. Cellular localization and adaptive changes of the cardiac delta opioid receptor system in an experimental model of heart failure in rats. Heart Vessels. 31: 241-50.

PMID: # 26897375
Nagasawa, A. et al. 2016. t-Flavanone Improves the Male Pattern of Hair Loss by Enhancing Hair-Anchoring Strength: A Randomized, Double-Blind, Placebo-Controlled Study. Dermatol Ther (Heidelb). 6: 59-68.

PMID: # 26160454
Fauzi, I. et al. 2016. In Vitro Differentiation of Embryonic Stem Cells into Hematopoietic Lineage: Towards Erythroid Progenitor's Production. Methods Mol. Biol.. 1341: 217-34.

Citations 1 to 10 of 905 total

What is in your RIPA buffer?

Asked by: Judy Crabtree
Thank you for your question. The RIPA buffer contains: 1x PBS, 1% Nonidet P-40 or Igepal CA-630, 0.5% sodium deoxycholate, 0.1% SDS. Add inhibitors at time of use from the following stock solutions. 1)10 mg/ml PMSF (sc-3597) in isopropanol (add at 10 µl/ml RIPA) 2)Aprotinin (sc-3595) (add at 50 KIU/ml RIPA) 3)100 mM sodium orthovanadate (sc-3540) in frozen aliquots (add at 10 µl/ml RIPA) Please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Tech Service
Date published: 2017-02-20

Can I order only PMSF, protease inhibitor cocktail and sodium orthovanadate in water? I have plenty of RIPA buffer but these molecules are almost consumed all?

Asked by: Ahmet
Thank you for your question. Yes, we sell each of these components of our RIPA Lysis Buffer System (sc-24948) separately. The product catalog numbers are listed below. And links to the product pages on our website are also provided for your reference. PMSF Solution: sc-482875 https://www.scbt.com/scbt/product/pmsf-solution-329-98-6?requestFrom=search UltraCruz® Protease Inhibitor Cocktail Tablet: sc-29130 https://www.scbt.com/scbt/product/ultracruz-protease-inhibitor-cocktail-tablet?requestFrom=search Sodium Orthovanadate: sc-3540 https://www.scbt.com/scbt/product/sodium-orthovanadate-13721-39-6?requestFrom=search
Answered by: Technical Support
Date published: 2017-02-13

Does it need to add DTT into the lysis system?

Asked by: zebrafish
Thank you for your question. We do not recommend adding DTT into the RIPA Lysis Buffer System (sc-24948) for sample preparation. We recommend adding DTT to SDS-PAGE electrophoresis sample buffer as a reducing agent if using the prepared cell lysates for Western blotting. A link is provided below to our Western blotting protocol, which also has a protocol for using the RIPA Lysis Buffer for sample preparation. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-02-06

Is there a full protocol for protein extraction using the RIPA Lysis Buffer System?

Asked by: Jz28sail
Thank you for your question. Please refer to the Technical Information tab on the product page, below the ordering information for usage instructions (summarized below). • Immediately prior to lysing cells, combine 10 μl PMSF solution, 10 μl sodium orthovanadate solution and 10-20 μl protease inhibitor cocktail solution per ml of 1X RIPA Lysis buffer to prepare complete RIPA. • Use 3 ml complete RIPA per gram of tissue - OR 1 ml complete RIPA per 2.0x107 cells in suspension - OR 0.6 ml complete RIPA per subconfluent monolayer on a 100 mm plate. For more detailed protocol information, please refer to the Sample Preparation section of our Western Blotting protocol (link below): https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-02-03

why is your RIPA lysis buffer light sensitive?

Asked by: stacy
Thank you for your question. The stock solution contains sodium deoxycholate which is light sensitive. In addition, according to the product SDS for our RIPA Lysis Buffer System (sc-24948), the component vials of PMSF and sodium orthovanadate are light sensitive and should be stored in a cool dark place.
Answered by: Technical Support
Date published: 2017-02-03

Is this protease inhibitor cocktail in the kit inhibit phosphatase?

Asked by: zebrafish
Thank you for your question. The only phosphatase inhibitor included in our RIPA Lysis Buffer System (sc-24948) is sodium orthovanadate. We recommend using one or more of our separate Phosphatase Inhibitor Cocktail(s) (sc-45044, sc-45045 and sc-45065) in combination with our RIPA Lysis Buffer System.
Answered by: Technical Support
Date published: 2017-01-27

Does this buffer system contain either beta glycerol phosphate or pyrophosphate?

Asked by: 13punMA

What is the pH of the RIPA buffer?

Asked by: 13punMA
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Rated 5 out of 5 by from Outstanding product I have been using this buffer system for last 15 years. It always worked for extraction process for different cell lines. Highly recommended.
Date published: 2017-02-06
Rated 5 out of 5 by from great In our experiment will often use this product, easy to operate, can get good results, the effect is very good!
Date published: 2017-02-06
Rated 5 out of 5 by from very good This system works good at our lab! I always buy this buffer.
Date published: 2017-01-23
Rated 4 out of 5 by from Great lysis system Works well for basic TC cell lysis. Compatible with BCA assay.
Date published: 2017-01-17
Rated 5 out of 5 by from function is good The product was used routinely in our lab .It works well.we will continue to use it in the future.
Date published: 2017-01-16
Rated 5 out of 5 by from Cheaper I bought this 3 months ago, It works great. easy using and good results.
Date published: 2016-12-07
Rated 5 out of 5 by from great functions I had used this product and it performed very well. It used routinely in our lab and had good quality.
Date published: 2016-11-19
Rated 5 out of 5 by from Works fine It s been our routine product and works very well.
Date published: 2016-11-01
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