RIPA Lysis Buffer System A high quality, ready to use mammalian cell and tissue lysis buffer with protease inhibitors included

RIPA Lysis Buffer System

RIPA Lysis Buffer System is rated 4.8 out of 5 by 25.
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| See product citations (932)
Application: A high quality, ready to use mammalian cell and tissue lysis buffer with protease inhibitors included
* Refer to Certificate of Analysis for lot specific data (including water content).
Submit a review for this product and receive 15 CruzCredits

Ordering Information

PRODUCT NAME CATALOG # UNIT PRICE QTY FAVORITES
RIPA Lysis Buffer System sc-24948 50 ml $29.00
RIPA Lysis Buffer System sc-24948A 500 ml $169.00

RIPA (Radioimmunoprecipitation) Lysis Buffer is used to lyse cells and tissue, for radio immunoprecipitation assay (RIPA). RIPA lysis buffer has stronger denaturing capabilities than NP-40 (sc-281108) or Triton X-100 (sc-29112) and is particularly useful for disruption of nuclear membranes in the preparation of nuclear extracts.

This high quality product includes protease inhibitors, making it ready for use in mammalian cell and tissue lysis.

sc-24948, 50 mL - Components supplied in four vials:
VIAL 1: 50 mL 1X lysis buffer (pH 7.4 ±0.1)
VIAL 2: 500 μL (200mM) PMSF in DMSO
VIAL 3: 500 μL protease inhibitor cocktail in DMSO
VIAL 4: 500 μL (100mM) sodium orthovanadate in water

sc-24948A, 500 mL - Components supplied in four vials:
VIAL 1: 500 mL 1X lysis buffer
VIAL 2: 5 ml (200mM) PMSF in DMSO
VIAL 3: 5 ml protease inhibitor cocktail in DMSO
VIAL 4: 5 ml (100mM) sodium orthovanadate in water


References

1. Poirier, F., et al. 1982. Int. J. Cancer. 29: 69-76. PMID: 6277805
2. DeSeau, V., et al. 1987. J. Cell. Biochem. 35: 113-128. PMID: 2448318
3. Bolen, J.B., et al. 1987. Oncogene Res. 1: 149-168. PMID: 2453014
4. Ngoka, L.C. 2008. Proteome Sci. 6: 30. PMID: 18950484

Usage :
• Immediately prior to lysing cells, combine 10 μl PMSF solution, 10 μl sodium orthovanadate solution and 10-20 μl protease inhibitor cocktail solution per ml of 1X RIPA Lysis buffer to prepare complete RIPA.
• Use 3 ml complete RIPA per gram of tissue
- OR 1 ml complete RIPA per 2.0x107 cells in suspension
- OR 0.6 ml complete RIPA per subconfluent monolayer on a 100 mm plate.
Physical State :
Liquid
Storage :
Store at -20° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Download SDS (MSDS)

Certificate of Analysis

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RIPA Lysis Buffer System Product Citations

See how others have used RIPA Lysis Buffer System. Click on the entry to view the PubMed entry .

Citations 1 to 10 of 932 total

PMID: # 28157110
Chen, W. et al. 2017. Connexin40 correlates with oxidative stress in brains of traumatic brain injury rats. Restor. Neurol. Neurosci.. 35: 217-224.

PMID: # 28551696
Wang, LJ. et al. 2017. Zinc Finger E-Box Binding Protein 2 (ZEB2) Suppress Apoptosis of Vascular Endothelial Cells Induced by High Glucose Through Mitogen-Activated Protein Kinases (MAPK) Pathway Activation. Med. Sci. Monit.. 23: 2590-2598.

PMID: # 28553222
Nair, RR. et al. 2017. Dynamic Arc SUMOylation and Selective Interaction with F-Actin-Binding Protein Drebrin A in LTP Consolidation In Vivo. Front Synaptic Neurosci. 9: 8.

PMID: # 28775096
Singh, B. et al. 2017. Repetitive Intradermal Bleomycin Injections Evokes T Helper Cell 2 Cytokine-driven Pulmonary Fibrosis. Am. J. Physiol. Lung Cell Mol. Physiol.. ajplung.00184.2017.

PMID: # 28099448
Fukuoka, CY. et al. 2017. The Effects of Low-Power Laser Irradiation on Inflammation and Apoptosis in Submandibular Glands of Diabetes-Induced Rats. PLoS ONE. 12: e0169443.

PMID: # 28534197
Shi, L. et al. 2017. PCMT1 Ameliorates Neuronal Apoptosis by Inhibiting the Activation of MST1 after Subarachnoid Hemorrhage in Rats. Transl Stroke Res.

PMID: # 28243322
García-Castillo, V. et al. 2017. Targeting Metabolic Remodeling in Triple Negative Breast Cancer in a Murine Model. J Cancer. 8: 178-189.

PMID: # 28099152
Jelinic, P. et al. 2017. The EMSY threonine 207 phospho-site is required for EMSYdriven suppression of DNA damage repair. Oncotarget. 8: 13792-13804.

PMID: # 28088228
Du, WY. et al. 2017. The loss-of-function mutations and down-regulated expression of ASB3 gene promote the growth and metastasis of colorectal cancer cells. Chin J Cancer. 36: 11.

PMID: # 28501331
Slusser-Nore, A. et al. 2017. The expression of keratin 6 is regulated by the activation of the ERK1/2 pathway in arsenite transformed human urothelial cells. Toxicol. Appl. Pharmacol..

Citations 1 to 10 of 932 total

I want to use Transglutaminase Assay Kit (sigma CS1070) to detect enzyme activity of cell lines. Can I use RIPA buffer from santa cruz to lysis cells?

Asked by: youyoumama
Thank you for your question. We would recommend that you contact the other supplier of the specific enzyme assay kit to inquire about the compatibility of the components in their kit with standard cell lysis buffers such as our RIPA Lysis Buffer System: sc-24948.
Answered by: Technical Support
Date published: 2017-08-30

Will it interfere with Bradford Assay?

Asked by: Elvira
Thank you for your question. No, the RIPA Lysis Buffer System does not contain substances that interfere with a Bradford assay. We do recommend including a positive control protein with a known concentration as a reference. Depending on the method and timing of your absorbance measurements, it is expected that you may still observe a difference of 10-20% in concentration.
Answered by: Technical Support
Date published: 2017-08-16

1st question: Could I use this Ripa buffer in Co-immunoprecipitation to study cytoplasmic protein-protein interaction? 2nd question: Could I use this Ripa buffer in Co-immunoprecipitation to study nuclear protein-protein interaction? any alternatives?

Asked by: Ibrahim
Thank you for your question. Our RIPA Lysis Buffer System (sc-24948) contains Sodium Dodecyl Sulfate (SDS) which is a detergent and may not be suitable for studying protein-protein interactions in a Co-Immunoprecipitation (Co-IP) experiment. We would recommend using lysing the cells under non-denaturing conditions to not disrupt the protein interactions. Unfortunately, we do not have a Co-IP protocol to share with you, but would recommend consulting the literature for more recommendations for Co-IP buffers.
Answered by: Technical Support
Date published: 2017-07-19

Is this buffer compatible with downstream masspec applications?

Asked by: membrane
Thank you for your question. The RIPA Lysis Buffer System: sc-24948 is for the release of cytoplasmic, membrane and nuclear proteins from adherent and suspension cultured mammalian cells. It is compatible with many applications including immunoassays and protein purification techniques. However, it may not be suitable for down stream mass spectrometry applications given the presence of SDS and other detergents. The complete product SDS is available to download on our website if you would like to view the formulation.
Answered by: Technical Support
Date published: 2017-03-07

Do any of the chemical components of the RIPA Lysis buffer system contain EDTA?

Asked by: 13punMA
Thank you for asking us about our RIPA Lysis Buffer System. There is no EDTA in any of the RIPA components.
Answered by: Chemical Support 8
Date published: 2017-03-03

What is the pH of the RIPA buffer?

Asked by: 13punMA
Thank you for asking about our RIPA Lysis Buffer System. The pH specification for the 1X RIPA buffer (50 ml bottle) in the RIPA kit is between pH 7.35 - 7.45.
Answered by: Chemical Support 8
Date published: 2017-03-03

Would you be able to tell me how much NaCl is in this buffer?

Asked by: 13punMA
Thank you for asking about our RIPA Lysis Buffer System. The vial that contains the 1X TBS contains NaCl at a concentration of 150 mM.
Answered by: Chemical Support 8
Date published: 2017-03-03

Does this contain substances that interfere BCA assay?

Asked by: ChemSynth123
Thank you for your question. No, RIPA Lysis Buffer System: sc-24948 does not contain substances that interfere with a BCA assay.
Answered by: Chemical Support 7
Date published: 2017-03-01
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Rated 5 out of 5 by from RIPA lysis buffer This RIPA lysis buffer system contains buffer, protease inhibitor cocktail, phosphatase inhibitors. Easy to use.
Date published: 2017-04-28
Rated 5 out of 5 by from Outstanding product I have been using this buffer system for last 15 years. It always worked for extraction process for different cell lines. Highly recommended.
Date published: 2017-02-06
Rated 5 out of 5 by from great In our experiment will often use this product, easy to operate, can get good results, the effect is very good!
Date published: 2017-02-06
Rated 5 out of 5 by from very good This system works good at our lab! I always buy this buffer.
Date published: 2017-01-23
Rated 4 out of 5 by from Great lysis system Works well for basic TC cell lysis. Compatible with BCA assay.
Date published: 2017-01-17
Rated 5 out of 5 by from function is good The product was used routinely in our lab .It works well.we will continue to use it in the future.
Date published: 2017-01-16
Rated 5 out of 5 by from Cheaper I bought this 3 months ago, It works great. easy using and good results.
Date published: 2016-12-07
Rated 5 out of 5 by from great functions I had used this product and it performed very well. It used routinely in our lab and had good quality.
Date published: 2016-11-19
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