Red Blood Cell (RBC) Lysis Buffer has been designed, formulated, and tested to ensure optimal lysis of RBCs in single cell suspensions with minimal effects on leukocytes.
RBC Lysis Buffer is supplied as a 10X solution containing ammonium chloride, potassium carbonate, and EDTA, and should be diluted in deionized water prior to use.
1. Adams, R.A., et al. 2007. J. Exp. Med. 204: 571-582. PMID: 17339406 2. Field-Corbett, C., et al. 2009. Stem Cells. 27: 662-669. PMID: 19096034
RBC lysis buffer is supplied as a 10X solution and should be diluted to 1X in deionized water. 100 ml of 10X concentrate will yield a quantity of 1X solution that is sufficient to lyse 500 samples.
The pH of the 1X solution should fall within the range of pH 7.1-7.4. Adjust the pH if necessary. Warm the 1X solution to room temperature prior to use.
NOTE: The blood sample must be <1 month old and been stored in anticoagulants (i.e. EDTA, heparin, and citrate).
1. Add whole blood to RBC Lysis Buffer at a ratio of approximately 1 part blood to 2 parts Lysis Buffer. Invert the tube to mix and incubate for 5 minutes at room temperature on a shaking platform or with periodic inversions. Do not vortex.
2. Centrifuge for 5 minutes then remove supernatant carefully without disturbing the pellet.
Note: If pellet is not white, repeat steps 1 and 2 with additional RBC Lysis Buffer.
3. If a lower red pellet is still visible below the white pellet then repeat steps 1 and 2 with fresh blood with the following modifications: a. Ensure the RBC Lysis Buffer is at room temperature. b. Increase the incubation to 15 minutes c. Use a higher ratio of RBC Lysis Buffer to blood (3:1).
Soluble in water.
Store at 4° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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RBC Lysis Buffer, 10X
See how others have used RBC Lysis Buffer, 10X. Click on the entry to view the PubMed entry
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Rated 5 out of
Very similar performance to other optionsWe compared to other available ACK and RBC lysis solutions. Very similar performance.
Date published: 2017-05-18
Rated 5 out of
Various publications cite the use of RBCVarious publications cite the use of RBC Lysis Buffer in their applications to remove red blood cells from samples. -SCBT Publication Review
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