Puromycin dihydrochloride An aminonuclease antibiotic used for selection and maintenance of cell lines expressing a transfected pac gene

Puromycin dihydrochloride (CAS 58-58-2)

  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 99ms
  • REVIEWS, PRODUCT
  • bvseo-msg: The resource to the URL or file is currently unavailable.;
同义词: Stylomycin hydrochloride
应用; An aminonuclease antibiotic used for selection and maintenance of cell lines expressing a transfected pac gene
CAS号码: 58-58-2
纯度: ≥98%
分子量: 544.43
分子式: C22H29N7O52HCl
* 参考分析证明 大量特定数据 (包括水 含量).
提交对此产品的评价,获得15个CruzCredits

Puromycin is an aminonuclease antibiotic used for selection and maintenance of cell lines expressing a transfected pac gene, whose product, puromycin-N-acetyl-transferase, inactivates puromycin via acetylation.
This product has been cell culture tested and is recommended for selection of stably transfected cells following transfection with Santa Cruz Biotechnology shRNA or Lentiviral particles.
BACKGROUND: Puromycin is an antibiotic substance produced by the soil actinomycete Streptomyces alboniger which induces apoptosis in cells by interfering with RNA function, leading to inhibition of protein synthesis. Toxic to both eukaryotic and prokaryotic cells. Believed to act as an acyl-tRNA analogue causing premature chain termination, it has been shown to arrest cells in G2/M phase.


参考文献

1. Brunetti, M., et al. 1995. Blood. 86: 4199-4205. PMID: 7492778
2. Constam, D.B., et al. 1995. J. Biol. Chem. 270: 26931-26939. PMID: 7592939
3. Sugita, M., et al. 1995. Zool. Sci. 12: 419-425. PMID: 8528014
4. Tabuchi, I. 2003. Biochem. Biophys. Res. Commun. 305: 1-5. PMID: 12732187

用法 :
For the selection of stably transfected cells following transfection with Santa Cruz Biotechnology shRNA or Lentiviral particles, proceed with puromycin selection as follows:

NOTE: The working puromycin concentration for selection in mammalian cell lines ranges from 1-10 µg/ml. Prior to using the puromycin antibiotic, titrate the selection agent to determine the optimal concentration for target cell line (see below). Use the lowest concentration that kills 100% of non-transfected cells in 3-5 days from the start of puromycin selection.


•48 hours post-transfection, aspirate the medium and replace with fresh medium containing puromycin at the appropriate concentration.

•Approximately every 2-3 days, aspirate and replace with freshly prepared selective media.

•Monitor the cells daily. Puromycin selection requires a minimum of 48 hours. Optimum effectiveness should be reached within 3-10 days.

•Assay transfected cells.

NOTE: Lot-to-lot variations in potency exist for all selection antibiotics, each new lot of puromycin should be titrated.
Titrating puromycin for selecting transfected cell lines:

•Plate 2 x 105 cells in each well of a 6-well plate containing 3 ml of the appropriate complete medium plus increasing concentrations of puromycin (i.e., 0, 1.0, 2.5, 5.0, 7.5, and 10.0 µg/ml)

•Replace with fresh selective medium after 2 days to remove dead cells.

•Examine the wells for viable cells every two days.

•Monitor the cells daily and observe the percentage of surviving cells. Optimum effectiveness should be reached in 1-4 days.

•The minimum antibiotic concentration to use is the lowest concentration that kills 100% of the cells in 3-5 days from the start of puromycin selection.
外观 :
Powder
物理状态 :
Solid
来源于 :
Streptomyces alboniger
溶解度 :
Soluble in water (50 mg/ml), ethanol (~1 mg/ml), DMSO (~13 mg/ml), DMF (~14 mg/ml), and PBS, pH 7.2 (~10 mg/ml).
保存 :
Desiccate at -20° C
熔点 :
178-180° C
仅供科研使用。不可用于诊断或治疗。
德国水公害等级 :
3
RTECS :
AU7355000
PubChem CID :
64806
默克索引 :
14: 7943
MDL 号码 :
MFCD00012691
EC号码 :
200-387-8
Beilstein 注册 :
3853613
SMILES :
CN(C)C1=NC=NC2=C1N=CN2[C@H]3[C@@H]([C@@H]([C@H](O3)CO)NC(=O)[C@@H](CC4=CC=C(C=C4)OC)N)O.Cl.Cl

下载SDS

分析证明书

在PDF文件上阅读,打印和批注,Adobe阅读器是必须的

Puromycin dihydrochloride  产品相关文献索引

看其他用户如何应用Puromycin dihydrochloride.点击进入浏览PubMed条目 .

引用1 - 10122

PMID: # 29035751  Zhang, S. et al. 2018. Neurobiol. Aging. 61: 52-65.

PMID: # 28849207  Li, ZY. et al. 2017. Mol Med Rep. 16: 5908-5914.

PMID: # 28168879  Kamarajan, P. et al. 2017. J. Proteome Res. 16: 1315-1326.

PMID: # 28230853  Yoo, HS. et al. 2017. Cell Death Dis. 8: e2632.

PMID: # 28152509  Yoshino, H. et al. 2017. Oncotarget. 8: 20881-20894.

PMID: # 27730520  Xu, J. et al. 2017. Appl. Biochem. Biotechnol. 181: 699-709.

PMID: # 28864500  Liu, Y. et al. 2017. Am. J. Physiol. Gastrointest. Liver Physiol. 313: G478-G491.

PMID: # 28367093  Hu, Y. et al. 2017. Int. J. Biol. Sci. 13: 286-294.

PMID: # 28450277  Saxena, A. et al. 2017. Am. J. Physiol. Gastrointest. Liver Physiol. 313: G26-G38.

PMID: # 28423652  Drago-Ferrante, R. et al. 2017. Oncotarget. 8: 28939-28958.

引用1 - 10122
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 76ms
  • QUESTIONS, PRODUCT
  • bvseo-msg: The resource to the URL or file is currently unavailable.;
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 50ms
  • REVIEWS, PRODUCT
  • bvseo-msg: The resource to the URL or file is currently unavailable.;

圣克鲁斯生物技术在生物医药研究市场的产品开发方面处于世界领先地位。欢迎 致电我们的免费电话1-800-457-3801。 版权 ©, 2007-2019圣克鲁斯生物技术。版权所有。“Santa Cruz Biotechnology”,圣克鲁斯生物技 术的 商标,“Santa Cruz Animal Health”,“San Juan Ranch”,“Supplement of Champions”,San Juan Ranch的商标,“Ultracruz”,“Chemcruz”,“Immunocruz”,“Exactacruz”和“EZ Touch”都是圣克鲁斯生物技术的注册商标。所有的商标都归其各自拥有者所有。