Puromycin dihydrochloride Puromycin is an aminonuclease antibiotic used for selection and maintenance of cell lines expressing a transfected pac gene.

Puromycin dihydrochloride  (CAS 58-58-2)

Puromycin dihydrochloride is rated 5.0 out of 5 by 12.
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| See 61 Citations
Synonym: Stylomycin hydrochloride
Application: An aminonuclease antibiotic used for selection and maintenance of cell lines expressing a transfected pac gene
CAS Number: 58-58-2
Purity: ≥98%
Molecular Weight: 544.43
Molecular Formula: C22H29N7O52HCl
* Refer to Certificate of Analysis for lot specific data (including water content).
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Ordering Information

PRODUCT NAME CATALOG # UNIT PRICE QTY FAVORITES
Puromycin dihydrochloride sc-108071 25 mg $30.00
Puromycin dihydrochloride sc-108071A 50 mg $55.00
Puromycin dihydrochloride sc-108071B 250 mg $167.00
Puromycin dihydrochloride sc-108071C 1 g $800.00

Puromycin is an aminonuclease antibiotic used for selection and maintenance of cell lines expressing a transfected pac gene, whose product, puromycin-N-acetyl-transferase, inactivates puromycin via acetylation.
This product has been cell culture tested and is recommended for selection of stably transfected cells following transfection with Santa Cruz Biotechnology shRNA or Lentiviral particles.
BACKGROUND: Puromycin is an antibiotic substance produced by the soil actinomycete Streptomyces alboniger which induces apoptosis in cells by interfering with RNA function, leading to inhibition of protein synthesis. Toxic to both eukaryotic and prokaryotic cells. Believed to act as an acyl-tRNA analogue causing premature chain termination, it has been shown to arrest cells in G2/M phase.


References

1. Brunetti, M., et al. 1995. Blood. 86: 4199-4205. PMID: 7492778
2. Constam, D.B., et al. 1995. J. Biol. Chem. 270: 26931-26939. PMID: 7592939
3. Sugita, M., et al. 1995. Zool. Sci. 12: 419-425. PMID: 8528014
4. Tabuchi, I. 2003. Biochem. Biophys. Res. Commun. 305: 1-5. PMID: 12732187

Usage :
For the selection of stably transfected cells following transfection with Santa Cruz Biotechnology shRNA or Lentiviral particles, proceed with puromycin selection as follows:
NOTE: The working puromycin concentration for selection in mammalian cell lines ranges from 1-10 µg/ml. Prior to using the puromycin antibiotic, titrate the selection agent to determine the optimal concentration for target cell line (see below). Use the lowest concentration that kills 100% of non-transfected cells in 3-5 days from the start of puromycin selection.
• 48 hours post-transfection, aspirate the medium and replace with fresh medium containing puromycin at the appropriate concentration.
• Approximately every 2-3 days, aspirate and replace with freshly prepared selective media.
• Monitor the cells daily. Puromycin selection requires a minimum of 48 hours. Optimum effectiveness should be reached within 3-10 days.
• Assay transfected cells.
NOTE: Lot-to-lot variations in potency exist for all selection antibiotics, each new lot of puromycin should be titrated.
Titrating puromycin for selecting transfected cell lines:
• Plate 2 x 105 cells in each well of a 6-well plate containing 3 ml of the appropriate complete medium plus increasing concentrations of puromycin (i.e., 0, 1.0, 2.5, 5.0, 7.5, and 10.0 µg/ml)
• Replace with fresh selective medium after 2 days to remove dead cells.
• Examine the wells for viable cells every two days.
• Monitor the cells daily and observe the percentage of surviving cells. Optimum effectiveness should be reached in 1-4 days.
• The minimum antibiotic concentration to use is the lowest concentration that kills 100% of the cells in 3-5 days from the start of puromycin selection.
Appearance :
Powder
Physical State :
Solid
Derived From :
Streptomyces alboniger
Solubility :
Soluble in water (50 mg/ml), ethanol (~1 mg/ml), DMSO (~13 mg/ml), DMF (~14 mg/ml), and PBS, pH 7.2 (~10 mg/ml).
Storage :
Desiccate at -20° C
Melting Point :
178-180 °C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
RTECS :
AU7355000
PubChem CID :
64806
Merck Index :
14: 7943
MDL Number :
MFCD00012691
EC Number :
200-387-8
Beilstein Registry :
3853613
SMILES :
CN(C)C1=NC=NC2=C1N=CN2[C@H]3[C@@H]([C@@H]([C@H](O3)CO)NC(=O)[C@@H](CC4=CC=C(C=C4)OC)N)O.Cl.Cl

Download SDS (MSDS)

Certificate of Analysis

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Puromycin dihydrochloride Product Citations

See how others have used Puromycin dihydrochloride. Click on the entry to view the PubMed entry .

Citations 1 to 10 of 61 total

PMID: # 27656108
Luján, P. et al. 2016. PRL-3 disrupts epithelial architecture by altering the post-mitotic midbody position. J. Cell. Sci.. 129: 4130-4142.

PMID: # 26934103
Schweingruber, C. et al. 2016. Identification of Interactions in the NMD Complex Using Proximity-Dependent Biotinylation (BioID). PLoS ONE. 11: e0150239.

PMID: # 27687248
Ilmer, M. et al. 2016. Low expression of galectin-3 is associated with poor survival in node-positive breast cancers and mesenchymal phenotype in breast cancer stem cells. Breast Cancer Res.. 18: 97.

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Li, S. et al. 2016. Ggnbp2 Is Essential for Pregnancy Success via Regulation of Mouse Trophoblast Stem Cell Proliferation and Differentiation. Biol. Reprod..

PMID: # 27607580
Klingbeil, O. et al. 2016. Inhibition of BET bromodomain-dependent XIAP and FLIP expression sensitizes KRAS-mutated NSCLC to pro-apoptotic agents. Cell Death Dis. 7: e2365.

PMID: # 26773497
Han, N. et al. 2016. MiR-328 suppresses the survival of esophageal cancer cells by targeting PLCE1. Biochem. Biophys. Res. Commun.. 470: 175-80.

PMID: # 27129263
Wu, Y. et al. 2016. Induction of Siglec-1 by endotoxin tolerance suppresses the innate immune response by promoting TGF-β1 production. J. Biol. Chem.. 291: 12370-82.

PMID: # 26183824
Kim, H. et al. 2015. Poly(ADP-ribose) polymerase as a novel regulator of 17β-estradiol-induced cell growth through a control of the estrogen receptor/IGF-1 receptor/PDZK1 axis. J Transl Med. 13: 233.

PMID: # 25636200
Bilal, MY. et al. 2015. GADS is required for TCR-mediated calcium influx and cytokine release, but not cellular adhesion, in human T cells. Cellular signalling. 27: 841-50.

PMID: # 26344763
Gabitova, L. et al. 2015. Endogenous Sterol Metabolites Regulate Growth of EGFR/KRAS-Dependent Tumors via LXR. Cell Rep. 12: 1927-38.

Citations 1 to 10 of 61 total

How long does Puromycin from santacruz lab takes to be delivered in Netherlands? If we are using HDR and KO plamsid from santacruz, then is it necessary to check with Cre vector?

Asked by: Mansi
Thank you for your question. For accurate availability, shipping and pricing information for Puromycin, please contact our European Customer Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: europe@scbt.com or by live chat directly on our website, www.scbt.com. Alternatively, you can contact our local distributor in Belgium, https://www.scbt.com/scbt/resources/distributors. It is not necessary to use the Cre vector to remove the insert from the HDR plasmid unless you wish to remove the inserted Puromycin from your cells. You would check the successful transfection and insertion by checking for GFP/RFP and Puromycin resistance. Please contact Technical Service at the contact information given above if you have any further questions.
Answered by: Tech Service
Date published: 2016-10-17

How long is this stable for in solution?

Asked by: hawkeye11

Can this product be stored in solution?

Asked by: hawkeye11

Is this product cell culture grade?

Asked by: hawkeye11

What is the shipping temperature for this product?

Asked by: hawkeye11
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Rated 5 out of 5 by from puromycin Puromycin used for cell line selection. it is very good
Date published: 2016-12-19
Rated 5 out of 5 by from Helps a lot The effect in my experiment is good using this product, fast screening results are stable, and easy to identify. It helps a lot in improving my experiment plans.
Date published: 2016-12-06
Rated 5 out of 5 by from GOOD FUCTION I used this for the cell screening and have a good fuction.
Date published: 2016-11-25
Rated 5 out of 5 by from You make me believe Used several times and it performs great. No complains
Date published: 2016-11-04
Rated 5 out of 5 by from Tested a small amount initially for CRISPR Tested a small amount initially for CRISPR experiments, got great results, purchased bulk amounts.
Date published: 2015-10-19
Rated 5 out of 5 by from We used it to select for KO in our cell line We used it to select for KO in our cell line and it worked really well.
Date published: 2015-10-12
Rated 5 out of 5 by from Worked well in our CRISPR transfection Worked well in our CRISPR transfection.
Date published: 2015-09-01
Rated 5 out of 5 by from Used it in KO work and works fine Used it in KO work and works fine, no complaints.
Date published: 2015-07-27
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