PTH/PTHrP-R Antibody (3D1.1)recommended for detection of PTH/PTHrP-R type I of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM

PTH/PTHrP-R Antibody (3D1.1): sc-12722

PTH/PTHrP-R Antibody (3D1.1) is rated 4.4 out of 5 by 11.
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Datasheets
    • PTH/PTHrP-R Antibody (3D1.1) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • epitope mapping within amino acids 155-169 of PTH/PTHrP-R of human origin
    • recommended for detection of PTH/PTHrP-R type I of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to TRITC for IF, IHC(P) and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PTH/PTHrP-R (3D1.1): sc-12722.
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I would like to use sc-12722 for some double staining. Any tips for using only mouse monoclonal antibodies?

Asked by: Cweed
We recommend using directly conjugated primary antibodies. This product, sc-12722, is available directly conjugated to FITC, AF488, and AF647.
Answered by: Technical Support 15
Date published: 2017-01-25
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Rated 4 out of 5 by from It's fine. PTH/PTHrP-R(3D1.1) expression in human urine(1:200 in 5%non-fat milk).
Date published: 2017-09-18
Rated 4 out of 5 by from Great result I used this product for western blot. It works good in Breast cancer cell lines.
Date published: 2017-06-01
Rated 5 out of 5 by from Excellent antibody The (3D1.1) clone is an excellent antibody with clean and specific results in WB and IHC(P). Very popular antibody with over a dozen product citations!
Date published: 2017-01-12
Rated 4 out of 5 by from Data published in: Cafforio P Data published in: Cafforio P, Savonarola A, Stucci S, De Matteo M, Tucci M, Brunetti AE, Vecchio VM, Silvestris F. PTHrP produced by myeloma plasma cells regulates their survival and pro-osteoclast activity for bone disease progression. J Bone Miner Res. 2014;29(1):55-66. doi: 10.1002/jbmr.2022.
Date published: 2015-10-27
Rated 4 out of 5 by from Publishable data in IP using human HEK293 cells Publishable data in IP using human HEK293 cells -SCBT Publication Review
Date published: 2015-07-18
Rated 5 out of 5 by from Great band detected in WB using Saos Great band detected in WB using Saos-2 whole cell lysate. -SCBT QC
Date published: 2015-03-31
Rated 4 out of 5 by from Worked well with transfected lysate by WB Worked well with transfected lysate by WB. -SCBT QC
Date published: 2015-03-18
Rated 4 out of 5 by from Publishable data in FCM using human Thymus Publishable data in FCM using human Thymus, tymocytes -SCBT Publication Review
Date published: 2015-03-04
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