Prickle1 Antibody (F-5) recommended for detection of Prickle1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Prickle1 Antibody (F-5): sc-393034

Prickle1 Antibody (F-5) is rated 5.0 out of 5 by 3.
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Datasheets
    • Prickle1 Antibody (F-5) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 685-766 mapping near the C-terminus of Prickle1 of human origin
    • recommended for detection of Prickle1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Prickle1 (F-5): sc-393034.
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I am using Prickle1 (F-5): sc-393034 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: AbPolly
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24

any evidence that this antibody will cross react with other prickle isoforms (prickle 2,3, or 4)? thanks Jason

Asked by: JasonJ
Thank you for your question. This antibody is specific for Prickle 1 and should not detect the other forms, 2, 3 or 4. Please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Tech Service
Date published: 2016-12-12
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Rated 5 out of 5 by from Great antibody for IHC(P) The Prickle1 Antibody (F-5): sc-393034 works very well for IHC when working with formalin fixed, paraffin-embedded tissue sections, showing staining localized to the cytoplasm and nucleus.
Date published: 2017-01-23
Rated 5 out of 5 by from Superb immunoperoxidase nuclear and cytoplasmic Superb immunoperoxidase nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded human placenta tissue. -SCBT QC
Date published: 2015-05-17
Rated 5 out of 5 by from Great Western Blot data of Prickle1 expression Great Western Blot data of Prickle1 expression in MDA-MB-231 and JAR whole cell lysates and human uterus tissue extract . -SCBT QC
Date published: 2013-06-26
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