Potassium phosphate tribasic monohydrateA reagent with very high buffering capacity

Potassium phosphate tribasic monohydrate (CAS 27176-10-9)

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Synonym: tert-Potassium phosphate monohydrate; Tripotassium phosphate
Application: A reagent with very high buffering capacity
CAS Number: 27176-10-9
Purity: ≥95%
Molecular Weight: 230.28
Molecular Formula: K3O4P•H2O
* Refer to Certificate of Analysis for lot specific data (including water content).
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Potassium phosphate tribasic monohydrate is a reagent with a very high buffering capacity. Widely used in molecular biology, biochemistry, and chromatography. Potassium phosphate occurs in several forms: monobasic (KH2PO4), dibasic (K2HPO4), and tribasic (K3PO4). Neutral potassium phosphate buffer solutions may be prepared with a mixture of the monobasic and dibasic forms to varying degrees, depending on the desired pH. Potassium Phosphate buffers are very useful in numerous applications, but with the following limitations; precipitation of Ca2+ and Mg2+, inhibition of restriction enzyme activity, and interference in DNA ligation or bacterial transformation protocols. Has been used to study the effects of freezing and thawing on the stability of proteins sensitive to conformational changes; it was found that KP buffers offered improved pH stability as opposed to NaP buffers. Potassium Phosphate has also been used for the extraction of keratohyalin protein from bovine tissue.
Additional forms available:
Potassium Phosphate, Dibasic, Anhydrous (sc-203210)
Potassium Phosphate, Monobasic (sc-203211)
Potassium phosphate tribasic (sc-250766)
Potassium phosphate dibasic solution (sc-301600)
Potassium Phosphate Monobasic solution (sc-301601)


References

1. Ugel, A.R. and Idler, W. 1972. J. Cell Biol. 52: 453-464. PMID: 5057980
2. Pikal-Cleland, K.A., et al. 2000. Arch. Biochem. Biophys. 384: 398-406. PMID: 11368330

Usage :
Neutral potassium phosphate buffer solutions are typically prepared by mixing of the monobasic and dibasic forms to varying degrees, depending on the desired pH.
Appearance :
Crystalline
Physical State :
Solid
pH :
12.5 - 14.0 at 230.3 g/l at 25° C
Storage :
Store at room temperature
Melting Point :
1340° C
Density :
2.564 g/cm3
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
RTECS :
TC8450000
PubChem CID :
22031701
MDL Number :
MFCD00150410
EC Number :
231-907-1
SMILES :
O.[O-]P(=O)([O-])[O-].[K+].[K+].[K+]

Download SDS (MSDS)

Certificate of Analysis

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What is this soluble in?

Asked by: chemicalsmg
Thank you for your question. This chemical is soluble in ethanol, methanol, DMF or DMSO. Poor water solubility. Please contact Technical Service if you have further questions concerning this product.
Answered by: Tech Service 9
Date published: 2017-01-18
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