Potassium phosphate dibasic solution A convenient 1M stock solution for making phosphate buffers

Potassium phosphate dibasic solution (CAS 7758-11-4)

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Application: A convenient 1M stock solution for making phosphate buffers
CAS Number: 7758-11-4
Molecular Weight: 174.18
Molecular Formula: HK2O4P
* Refer to Certificate of Analysis for lot specific data (including water content).
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Potassium phosphate dibasic solution is a convenient stock solution for making phosphate buffers. Widely used in molecular biology, biochemistry, and chromatography. Potassium phosphate occurs in several forms: monobasic (KH2PO4), dibasic (K2HPO4), and tribasic (K3PO4). Neutral potassium phosphate buffer solutions may be prepared with a mixture of the monobasic and dibasic forms to varying degrees, depending on the desired pH. Potassium phosphate buffers are very useful in numerous applications, but with the following limitations; precipitation of Ca2+ and Mg2+, inhibition of restriction enzyme activity, and interference in DNA ligation or bacterial transformation protocols. Has been used to study the effects of freezing and thawing on the stability of proteins sensitive to conformational changes; it was found that potassium phosphate buffers offered improved pH stability as opposed to sodium phosphate buffers. Potassium phosphate has also been used for the extraction of keratohyalin protein from bovine tissue.
Additional forms available:
Potassium Phosphate, Dibasic, Anhydrous (sc-203210)
Potassium Phosphate, Monobasic (sc-203211)
Potassium phosphate tribasic (sc-250766)
Potassium phosphate tribasic monohydrate (sc-250767)
Potassium Phosphate Monobasic solution (sc-301601)


References

1. Ugel, A.R. and Idler, W. 1972. J. Cell Biol. 52: 453-464. PMID: 5057980
2. Pikal-Cleland, K.A., et al. 2000. Arch. Biochem. Biophys. 384: 398-406. PMID: 11368330
3. Sambrook, J. and Russell, D.W. 2001. Molecular Cloning: A Laboratory Manual, 3rd ed. Cold Spring Harbor, NY. CSHL Press.

Usage :
Potassium phosphate dibasic solution is prepared with high-quality reagents and 0.2 μm filtered; however it has not been evaluated for use in cell culture. Please employ appropriate sterilization techniques for the intended application.
Formulation :
1.0 M
Physical State :
Liquid
pH :
8.5 - 9.0
Storage :
Store at room temperature
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
MDL Number :
MFCD00149926
EC Number :
231-834-5

Download SDS (MSDS)

Certificate of Analysis

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