Potassium Phosphate, Dibasic, Anhydrous A reagent with very high buffering capacity widely used in molecular biology

Potassium Phosphate, Dibasic, Anhydrous (CAS 7758-11-4)

Potassium Phosphate, Dibasic, Anhydrous | CAS 7758-11-4 is rated 5.0 out of 5 by 2.
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Synonym: Dipotassium hydrogenphosphate; Dipotassium phosphate; sec.-Potassium phosphate
Application: A reagent with very high buffering capacity widely used in molecular biology
CAS Number: 7758-11-4
Purity: ≥98%
Molecular Weight: 174.18
Molecular Formula: K2HPO4
* Refer to Certificate of Analysis for lot specific data (including water content).
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Potassium Phosphate, Dibasic, Anhydrous (K2HPO4) is a reagent with a very high buffering capacity. Widely used in molecular biology, biochemistry, and chromatography. Potassium phosphate occurs in several forms: monobasic (KH2PO4), dibasic (K2HPO4), and tribasic (K3PO4). Neutral potassium phosphate buffer solutions may be prepared with a mixture of the monobasic and dibasic forms to varying degrees, depending on the desired pH. Potassium Phosphate buffers are very useful in numerous applications, but with the following limitations: precipitation of Ca2+ and Mg2+, inhibition of restriction enzyme activity, and interference in DNA ligation or bacterial transformation protocols. Has been used to study the effects of freezing and thawing on the stability of proteins sensitive to conformational changes; it was found that KP buffers offered improved pH stability as opposed to NaP buffers. Potassium Phosphate has also been used for the extraction of keratohyalin protein from bovine tissue.
Additional forms available:
Potassium Phosphate, Dibasic, Anhydrous (sc-203210)
Potassium Phosphate, Monobasic (sc-203211)
Dipotassium hydrogen phosphate (sc-491980)
Potassium phosphate tribasic (sc-250766)
Potassium phosphate tribasic monohydrate (sc-250767)
Potassium phosphate dibasic solution (sc-301600)
Potassium Phosphate Monobasic solution (sc-301601)


References

1. Ugel, A.R. and Idler, W. 1972. J. Cell Biol. 52: 453-464. PMID: 5057980
2. Pikal-Cleland, K.A., et al. 2000. Arch. Biochem. Biophys. 384: 398-406. PMID: 11368330
3. Sambrook, et al. 1989. Rnai: Molecular Cloning: A Laboratory Manual. 2nd ed. Cold Spring Harbor Laboratory Press. New York. Cold Spring Harbor. Vol. 3.

Appearance :
Powder
Physical State :
Solid
Solubility :
Soluble in water (1600 g/l) at 20° C, and ethanol (Slightly soluble).
Storage :
Desiccate at room temperature
Melting Point :
340° C
Boiling Point :
158° C at 760 mmHg (Predicted)
Density :
2.3 g/cm3
pK Values :
pKa: 1.97, pK1: 2.15(25° C), pK2: 6.82(25° C), pK3: 12.38(25° C)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
RTECS :
TC5580000
PubChem CID :
24450
Merck Index :
14: 7658
MDL Number :
MFCD00011383
EC Number :
231-834-5
SMILES :
OP(=O)([O-])[O-].[K+].[K+]

Download SDS (MSDS)

Certificate of Analysis

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Potassium Phosphate, Dibasic, Anhydrous  Product Citations

See how others have used Potassium Phosphate, Dibasic, Anhydrous. Click on the entry to view the PubMed entry .

Citations 1 to 1 of 1 total

PMID: # 6371541  Morace, G. et al. 1984. Mycopathologia. 84: 81-85.

Citations 1 to 1 of 1 total

Need food grade Kosher/Halal certified. Your best quote, MOQ and lead time for these products as Mfgr. 1 1850kg of KH2PO4 (Potassium phosphate dibasic, powder) 2. 1000kg of UDP (Uridine 5'-diphosphate disodium salt, CAS 27821-45-0)

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Rated 5 out of 5 by from Easy Purchase + Fast Delivery=Amazing Transaction! Purchased this ingredient for a production batch that needed to be manufactured ASAP. Ordering was quick and next day shipping was included. Ingredient was of high quality. Excellent transaction overall.
Date published: 2017-03-21
Rated 5 out of 5 by from Pikal Pikal-Cleland et al. (PubMed ID 11368330) found that proteins buffered with a potassium phosphate solution were more resistant to pH changes resulting from freezing than those buffered with a sodium phosphate alternative. -SCBT Publication Review
Date published: 2015-07-12
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