Ponceau S solution A rapid protein band stain

Ponceau S solution (CAS 6226-79-5 (solid))

Ponceau S solution | CAS 6226-79-5 (solid) is rated 4.7 out of 5 by 3.
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Application: A rapid protein band stain
CAS Number: 6226-79-5 (solid)
Molecular Weight: 760.57
Molecular Formula: C22H12N4Na4O13S4
* Refer to Certificate of Analysis for lot specific data (including water content).
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Ponceau S stain comes ready-to-use and is designed for rapid (5 min) staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. Ponceau S staining on nitrocellulose and PVDF is reversible by washing the stained membrane with 0.1 M NaOH for 1 min. The stained and destained membrane can then be immunologically detected (Western blotting). Ponceau S is a negative stain which binds to the positively charged amino groups of the protein. It also binds non-covalently to non-polar regions in the protein. (Note: Ponceau S is not suitable for use with nylon membranes.)


References

1. Reinhart, M.P. and Malamud, D. 1982. Anal. Biochem. 123(2): 229-235. PMID: 6181706

Formulation :
0.1 % (w/v) in 5% acetic acid
Physical State :
Liquid
Solubility :
Soluble in water (1 mg/ml). Insoluble in ether.
Storage :
Store at room temperature
Refractive Index :
n20D 1.34
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
1
PubChem CID :
6364532
MDL Number :
MFCD00003892
EC Number :
228-319-2
SMILES :
C1=CC(=CC=C1N=NC2=CC(=C(C=C2)NN=C3C4=C(C=C(C=C4)S(=O)(=O)[O-])C=C(C3=O)S(=O)(=O)[O-])S(=O)(=O)[O-])S(=O)(=O)[O-].[Na+].[Na+].[Na+].[Na+]

Download SDS (MSDS)

Certificate of Analysis

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Ponceau S solution  Product Citations

See how others have used Ponceau S solution. Click on the entry to view the PubMed entry .

Citations 1 to 4 of 4 total

PMID: # 27486747  Avin, KG. et al. 2016. PLoS ONE. 11: e0159411.

PMID: # 27421685  Grill, A. et al. 2016. Acta Physiol (Oxf). 218: 198-211.

PMID: # 26100803  Tsai, YH. et al. 2015. Nat Chem. 7: 554-61.

PMID: # 19070893  Holdsworth-Carson, S.J. et al. 2009. Placenta. 30: 176-186.

Citations 1 to 4 of 4 total

What is the concentration of acetic acid in this Ponceau S solution?

Asked by: SCM4
In Ponceau S solution, sc-301558, the v/v concentration of acetic acid is 5%, but the w/v of acetic acid is approximately 1%.
Answered by: Tech Service 11
Date published: 2017-12-10
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Rated 4 out of 5 by from Great I bought this a month ago and by this time had use it for several times. I am happy with this product.
Date published: 2017-10-24
Rated 5 out of 5 by from Works great We use this to stain protein on PVDF membrane after transfer. Works very well.
Date published: 2017-07-14
Rated 5 out of 5 by from Use this routinely for WB and works well Use this routinely for WB and works well, no complaints.
Date published: 2015-12-16
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