Ponceau S stain comes ready-to-use and is designed for rapid (5 minutes) staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. Ponceau S staining on nitrocellulose and PVDF is reversible by washing the stained membrane with 0.1 M NaOH for 1 min. The stained and destained membrane can then be immunologically detected (Western blotting). Ponceau S is a negative stain which binds to the positively charged amino groups of the protein. It also binds non-covalently to non-polar regions in the protein. (Note: Ponceau S is not suitable for use with nylon membranes.)
1. Reinhart, M.P. and Malamud, D. 1982. Anal. Biochem. 123(2): 229-235. PMID: 6181706
See how others have used Ponceau S solution. Click on the entry to view the PubMed entry .
PMID: # 30914243 Sander, H.|Wallace, S.|Plouse, R.|Tiwari, S.|Gomes, AV.| et al. 2019. Anal. Biochem. 575: 44-53.
PMID: # 31350351 Fischer, J.|Gutièrrez, S.|Ganesan, R.|Calabrese, C.|Ranjan, R.|Cildir, G.|Hos, NJ.|Rybniker, J.|Wolke, M.|Fries, JWU.|Tergaonkar, V.|Plum, G.|Antebi, A.|Robinson, N.| et al. 2019. Proc. Natl. Acad. Sci. U.S.A.
PMID: # 27486747 Avin, KG. et al. 2016. PLoS ONE. 11: e0159411.
PMID: # 27421685 Grill, A. et al. 2016. Acta Physiol (Oxf). 218: 198-211.
PMID: # 26100803 Tsai, YH. et al. 2015. Nat Chem. 7: 554-61.
PMID: # 19070893 Holdsworth-Carson, S.J. et al. 2009. Placenta. 30: 176-186.
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