Polybrene sup A cationinc polymer used for infection of certain cells with a retrovirus in cell culture

Polybrene®

Polybrene | CAS 28728-55-4 is rated 5.0 out of 5 by 18.
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Datasheets
    • 1 ml; 10mg/ml in sterile water
    • highly efficient infection reagent used to introduce retroviral vectors into mammalian cells
    • also known as hexamethrine bromide, 1,5-Dimethyl-1,5-diazaundecamethylene polymethobromide
    • Polybrene® is a registered trademark of Abbott Laboratories Corp.
    • Store at -20º C
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Hello, I would like to know how long this product could be stable at -20C. I have 2 tubes of this product (lot #K1116, C0317), could you tell me when they were produced? Thanks, Carmen Gagnon

Asked by: Carmen G
We do not have data on the stability and long term storage for Polybrene® at -20C. We do warranty the product for one year from the date of shipment. If it was purchased more than one year ago, to be safe we would recommend purchasing a new lot.
Answered by: Technical Support
Date published: 2019-05-03

Is the material sterile and which are the method of sterilization?

Asked by: Anaaa
Thank you for your question. Yes, Polybrene ®: sc-134220, is provided as 10mg/ml in 1 ml sterile water. The product is prepared with MQ water, filtered via 0.22 um membrane, and vialed under sterile conditions in a hood. Please contact our Technical Service Department if you have further questions.
Answered by: Technical Support
Date published: 2018-07-27

Can I have an MSDS to Polybrene sc-134220?

Asked by: mazdog
Thank you for your question. The Material Safety Data Sheet for our Polybrene®: sc-134220 is available on our website using the "Datasheets" link. The link below will open the PDF. https://datasheets.scbt.com/sc-134220.pdf
Answered by: Technical Support
Date published: 2017-07-21

Is the product sterile?

Asked by: chemicalsmg
Thank you for your question. Polybrene ®, sc-134220, is a sterile product.
Answered by: TechService7
Date published: 2017-04-06
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Rated 5 out of 5 by from recommend for lab Used in sirRNA tracfection,got expected result,very reliable quality.
Date published: 2018-01-27
Rated 5 out of 5 by from 功能很出色 我很久以前购买了这个产品,感到很高兴,就是价格太高,没有很好的优惠活动。订购体系也很混乱,不是很方便
Date published: 2017-12-13
Rated 5 out of 5 by from Excellent transduction Got very poor lentiviral transduction efficiency in U937 monocytic cells, but adding polybrene 4 ug/mL dramatically improved efficiency in this cell line. Would recommend if you are struggling with low efficiency transductions. Just make sure to change the medium the following day to reduce cytotoxicity.
Date published: 2017-08-26
Rated 5 out of 5 by from レトロウイルスを用いた遺伝子導入の効率up レトロウイルスを用いた遺伝子導入に本製品を利用しました。遺伝子導入効率が本製品を使わない時に比べて、飛躍的に遺伝子導入効率が亢進しました。
Date published: 2017-08-21
Rated 5 out of 5 by from Great Although I haven't compared side-by-side conditions with and without polybrene for lenti/retroviral transduction, but by using it at 5-10 µg/mL for most cell types, including primary mouse bone marrow cells and HUVECs, I can get 50-100% transduction efficiency, depending on cell types.
Date published: 2017-08-02
Rated 5 out of 5 by from shRNA Used in shRNA experiments,got good results,often use this product, good quality.
Date published: 2016-12-18
Rated 5 out of 5 by from works well for the lenti transfection I bought a month ago and found it woks well .good for lenti transfections
Date published: 2016-11-17
Rated 5 out of 5 by from 使用非常好 因为实验需要,每隔一段时间都会购买,订购方便,价格便宜,到货非常快,而且性能稳定,从来没有出过质量问题,让我的实验顺利进行。
Date published: 2016-11-01
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