PNPP, Disodium Salt An alkaline phosphatase substrate used in ELISA applications; absorbs light at 405nm

PNPP, Disodium Salt  (CAS 4264-83-9)

PNPP, Disodium Salt | CAS 4264-83-9 is rated 5.0 out of 5 by 1.
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| See product citations (13)
Synonym: 4-Nitrophenyl phosphate disodium salt hexahydrate; p-Nitrophenyl phosphate disodium hexahydrate; Disodium 4-nitrophenyl phosphate hexahydrate; pNPP-2Na hexahydrate
Application: An alkaline phosphatase substrate used in ELISA applications; absorbs light at 405nm
CAS Number: 4264-83-9
Purity: ≥99%
Molecular Weight: 371.14
Molecular Formula: C6H4NO6P•2Na•6H2O
* Refer to Certificate of Analysis for lot specific data (including water content).
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Ordering Information

PRODUCT NAME CATALOG # UNIT PRICE QTY FAVORITES
PNPP, Disodium Salt sc-3720 25 g $75.00
PNPP, Disodium Salt sc-3720A 250 g $300.00

A colorimetric alkaline phosphatase soluble substrate, p-Nitrophenyl Phosphate (pNPP) is the substrate of choice for use with alkaline phosphatase in Enzyme Linked Immunosorbant Assay (ELISA) procedures. The reaction produces a soluble end product that is yellow in color. Can be used to perform kinetic and endpoint determinations. Also used as a phosphorylating agent in nucleotide synthesis. Recommended for use with pNPP Substrate Buffer (sc-296099). Also available in solution form: p-Nitrophenyl Phosphate 50X solution (sc-358718).


References

1. Voller, A., et al. 1976. Bull. World Health Organ. 53: 55-65. PMID: 1085667
2. Engvall, E. 1980. Meth. Enzymol. 70: 419-439. PMID: 6999296
3. Voller, A., et al. 1986. Manual of Clinical Laboratory Immunology, 3rd Edition. In Rose, N., et al. eds., Washington, D.C. American Society for Microbiology, 106-107.

Usage :
• To prepare PNPP substrate solution, dissolve 10 mg of room-temperature PNPP, Disodium Salt (sc-3720) into 10 ml of pNPP Substrate Buffer (sc-296099). Mix well and protect from light. Diluted product may be used for up to 10 hours following mixing. Discard if solution becomes yellow or turbid.
• Following incubation of antibody samples and the addition of alkaline phosphatase labeled probes, wash plates thoroughly avoiding the use of phosphate buffers. Add 100 µl of 1X PNPP substrate solution to each well and incubate 15-60 minutes. Incubation times will vary depending on the enzyme present.
• For endpoint analysis, the reaction may be stopped with 3N NaOH and read at 405 nm.
Appearance :
Crystalline powder
Physical State :
Solid
Solubility :
Soluble in water(freely soluble). Insoluble in ethanol, and acetone.
Storage :
Store at -20° C
Melting Point :
>300° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
PubChem CID :
77949
MDL Number :
MFCD00066288
EC Number :
224-246-5
Beilstein Registry :
5324661
SMILES :
C1=CC(=CC=C1[N+](=O)[O-])OP(=O)([O-])[O-].O.O.O.O.O.O.[Na+].[Na+]

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PNPP, Disodium Salt Product Citations

See how others have used PNPP, Disodium Salt. Click on the entry to view the PubMed entry .

Citations 1 to 10 of 13 total

PMID: # 23831060
Rodríguez-Torres, AM. et al. 2013. Structurally conserved and functionally divergent yeast Ssu72 phosphatases. FEBS Lett.. 587: 2617-22.

PMID: # 19546526
Chen, X. et al. 2009. Ischemic Preconditioning Attenuates Renal Ischemia-Reperfusion Injury by Inhibiting Activation of IKKbeta and Inflammatory Response. Am. J. Nephrol.. 30: 287-294.

PMID: # 19144997
Gava, E. et al. 2009. Angiotensin-(1-7) activates a tyrosine phosphatase and inhibits glucose-induced signalling in proximal tubular cells. Nephrol. Dial. Transplant.. 24: 1766-73.

PMID: # 19122201
Iuliano, R. et al. 2009. The eighth fibronectin type III domain of protein tyrosine phosphatase receptor J influences the formation of protein complexes and cell localization. J. Biochem.. 145: 377-385.

PMID: # 18976718
Baek, JA. et al. 2009. Extracts of Magnoliae flos inhibit inducible nitric oxide synthase via ERK in human respiratory epithelial cells. Nitric Oxide. 20: 122-128.

PMID: # 19142636
Gao, P. et al. 2009. Secretion of stress protein grp170 promotes immune-mediated inhibition of murine prostate tumor. Cancer Immunol. Immunother.. 58: 1319-28.

PMID: # 18694935
Mark, JK. et al. 2008. Inhibition of mitogen-activated protein kinase phosphatase 3 activity by interdomain binding. J. Biol. Chem.. 283: 28574-28583.

PMID: # 15929988
Ljusberg, J. et al. 2005. Proteolytic excision of a repressive loop domain in tartrate-resistant acid phosphatase by cathepsin K in osteoclasts. J. Biol. Chem.. 280: 28370-28381.

PMID: # 15890660
Kutuzov, MA. et al. 2005. Regulation of apoptosis signal-regulating kinase 1 (ASK1) by polyamine levels via protein phosphatase 5. J. Biol. Chem.. 280: 25388-25395.

PMID: # 15284227
Castelli, Marco et al. 2004. MAP Kinase Phosphatase 3 (MKP3) Interacts with and Is Phosphorylated by Protein Kinase CK2 J. Biol. Chem. 279: 44731-44739.

Citations 1 to 10 of 13 total

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Asked by: kkim123
Thank you for question. A colorimetric alkaline phosphatase soluble substrate, p-Nitrophenyl Phosphate (pNPP) is the substrate of choice for use with alkaline phosphatase in Enzyme Linked Immunosorbant Assay (ELISA) procedures. Please contact our Technical Service Department by phone, e-mail or live chat for further detail. 문의 감사합니다. p-Nitrophenyl Phosphate (pNPP) 는 알칼리 포스파타아제의 기질로서 ELISA반응에 쓰이는 알칼리 포스파타아제와 함께 반응하여 색을 나타내는 기질이며 단독염색이 가능한 염색물질이 아닙니다. 더 자세한 정보를 원하시면 저희 기술지원팀에 전화, 이메일또는 온라인채팅으로 연락하기 바랍니다.
Answered by: Technical Support 13
Date published: 2017-02-28
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Rated 5 out of 5 by from Gava et Gava et. al. (PubMed ID 19144997) used pNPP to measure the change in protein tyrosine phosphatase activity. The rate of formation of p-Nitrophenol from pNPP was measured by spectroscopy at 410nm. -SCBT Publication Review
Date published: 2015-04-03
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