A colorimetric alkaline phosphatase soluble substrate, p-Nitrophenyl Phosphate (pNPP) is the substrate of choice for use with alkaline phosphatase in Enzyme Linked Immunosorbant Assay (ELISA) procedures. The reaction produces a soluble end product that is yellow in color. Can be used to perform kinetic and endpoint determinations. Also used as a phosphorylating agent in nucleotide synthesis. Recommended for use with pNPP Substrate Buffer (sc-296099). Also available in solution form: p-Nitrophenyl Phosphate 50X solution (sc-358718).
1. Voller, A., et al. 1976. Bull. World Health Organ. 53: 55-65. PMID: 1085667
2. Engvall, E. 1980. Meth. Enzymol. 70: 419-439. PMID: 6999296
3. Voller, A., et al. 1986. Manual of Clinical Laboratory Immunology, 3rd Edition. In Rose, N., et al. eds., Washington, D.C. American Society for Microbiology, 106-107.
See how others have used PNPP, Disodium Salt. Click on the entry to view the PubMed entry .
PMID: # 23831060
Rodríguez-Torres, AM. et al. 2013. Structurally conserved and functionally divergent yeast Ssu72 phosphatases. FEBS Lett.. 587: 2617-22.
PMID: # 19546526
Chen, X. et al. 2009. Ischemic Preconditioning Attenuates Renal Ischemia-Reperfusion Injury by Inhibiting Activation of IKKbeta and Inflammatory Response. Am. J. Nephrol.. 30: 287-294.
PMID: # 19144997
Gava, E. et al. 2009. Angiotensin-(1-7) activates a tyrosine phosphatase and inhibits glucose-induced signalling in proximal tubular cells. Nephrol. Dial. Transplant.. 24: 1766-73.
PMID: # 19122201
Iuliano, R. et al. 2009. The eighth fibronectin type III domain of protein tyrosine phosphatase receptor J influences the formation of protein complexes and cell localization. J. Biochem.. 145: 377-385.
PMID: # 18976718
Baek, JA. et al. 2009. Extracts of Magnoliae flos inhibit inducible nitric oxide synthase via ERK in human respiratory epithelial cells. Nitric Oxide. 20: 122-128.
PMID: # 19142636
Gao, P. et al. 2009. Secretion of stress protein grp170 promotes immune-mediated inhibition of murine prostate tumor. Cancer Immunol. Immunother.. 58: 1319-28.
PMID: # 18694935
Mark, JK. et al. 2008. Inhibition of mitogen-activated protein kinase phosphatase 3 activity by interdomain binding. J. Biol. Chem.. 283: 28574-28583.
PMID: # 15929988
Ljusberg, J. et al. 2005. Proteolytic excision of a repressive loop domain in tartrate-resistant acid phosphatase by cathepsin K in osteoclasts. J. Biol. Chem.. 280: 28370-28381.
PMID: # 15890660
Kutuzov, MA. et al. 2005. Regulation of apoptosis signal-regulating kinase 1 (ASK1) by polyamine levels via protein phosphatase 5. J. Biol. Chem.. 280: 25388-25395.
PMID: # 15284227
Castelli, Marco et al. 2004. MAP Kinase Phosphatase 3 (MKP3) Interacts with and Is Phosphorylated by Protein Kinase CK2 J. Biol. Chem. 279: 44731-44739.
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