PLZF Antibody (D-9)
  • PLZF Antibody (D-9) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
  • raised against amino acids 101-400 of PLZF of human origin
  • recommended for detection of PLZF of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
  • available conjugated to HRP (sc-28319 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; and to either phycoerythrin (sc-28319 PE) or fluoroscein (sc-28319 FITC), Alexa Fluor® 488 (sc-28319 AF488) or Alexa Fluor® 647 (sc-28319 AF647), 200 µg/ml, for IF, IHC(P) and FCM
  • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP): sc-516102, our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
  • Achieve better research results using superior monoclonal antibodies from Santa Cruz! Contact us to receive a FREE 10 µg sample of PLZF (D-9): sc-28319.

PLZF Antibody (D-9): sc-28319

PLZF Antibody (D-9) is rated 4.9 out of 5 by 16.
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Datasheets
    • PLZF Antibody (D-9) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 101-400 of PLZF of human origin
    • recommended for detection of PLZF of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • available conjugated to HRP (sc-28319 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; and to either phycoerythrin (sc-28319 PE) or fluoroscein (sc-28319 FITC), Alexa Fluor® 488 (sc-28319 AF488) or Alexa Fluor® 647 (sc-28319 AF647), 200 µg/ml, for IF, IHC(P) and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP): sc-516102, our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Achieve better research results using superior monoclonal antibodies from Santa Cruz! Contact us to receive a FREE 10 µg sample of PLZF (D-9): sc-28319.
Submit a review for this product and receive 15 CruzCredits

Ordering Information

PRODUCT NAME CATALOG # UNIT PRICE QTY FAVORITES
PLZF Antibody (D-9) sc-28319 200 µg/ml $279.00
PLZF Antibody (D-9) Alexa Fluor® 488 sc-28319 AF488 200 µg/ml $314.00
PLZF Antibody (D-9) Alexa Fluor® 647 sc-28319 AF647 200 µg/ml $314.00
PLZF Antibody (D-9) FITC sc-28319 FITC 200 µg/ml $292.00
PLZF Antibody (D-9) HRP sc-28319 HRP 200 µg/ml $279.00
PLZF Antibody (D-9) PE sc-28319 PE 200 µg/ml $303.00

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PLZF Antibody (D-9) Product Citations

See how others have used PLZF Antibody (D-9). Click on the entry to view the PubMed entry .

Citations 1 to 10 of 71 total

PMID: # 27431324
Xu, Z. et al. 2016. H2B ubiquitination regulates meiotic recombination by promoting chromatin relaxation. Nucleic Acids Res..

PMID: # 26986811
Liu, C. et al. 2016. Autophagy is required for ectoplasmic specialization assembly in Sertoli cells. Autophagy. 0.

PMID: # 26857623
Le, W. et al. 2016. Low-dose ionizing irradiation triggers a 53BP1 response to DNA double strand breaks in mouse spermatogonial stem cells. Syst Biol Reprod Med. 62: 106-13.

PMID: # 27346680
Wang, S. et al. 2016. Retinoic Acid Is Sufficient for the In Vitro Induction of Mouse Spermatocytes. Stem Cell Reports. 7: 80-94.

PMID: # 26647924
Niu, YR. et al. 2016. Amodiaquine-induced reproductive toxicity in adult male rats. Mol. Reprod. Dev.. 83: 174-82.

PMID: # 26858254
Liu, YJ. et al. 2016. Degradation of the Separase-cleaved Rec8, a Meiotic Cohesin Subunit, by the N-end Rule Pathway. J. Biol. Chem.. 291: 7426-38.

PMID: # 27353491
Bai, Y. et al. 2016. Differential gene expression in mouse spermatogonial stem cells and embryonic stem cells. Int. J. Mol. Med.. 38: 423-32.

PMID: # 25611385
Gao, X. et al. 2015. A G-quadruplex DNA structure resolvase, RHAU, is essential for spermatogonia differentiation. Cell Death Dis. 6: e1610.

PMID: # 26265072
Hu, X. et al. 2015. Deletion of the tyrosine phosphatase Shp2 in Sertoli cells causes infertility in mice. Sci Rep. 5: 12982.

PMID: # 25687282
Dobenecker, MW. et al. 2015. Coupling of T cell receptor specificity to natural killer T cell development by bivalent histone H3 methylation. J. Exp. Med.. 212: 297-306.

Citations 1 to 10 of 71 total

Is this antibody can be used for Flow cytometry. If yes then what is the dilution. Do you also have a small pack of this antibody.

Asked by: ashish79
Thank you for your question. Yes, we are aware of several publications citing use of this PLZF Antibody (D-9): sc-28319 in flow cytometry (FCM). For example, see PMID: 22473038. We recommend using 1 µg of fluorochrome-conjugated antibodies in 100 µl of the prepared cell suspension (equal to 1 million cells) to each tube. A link to our recommended protocol is below as a reference. https://www.scbt.com/scbt/resources/protocols/flow-cytometry We are currently offering a free 10 µg sample size of the unconjugated primary antibody. Please contact our Technical Service Department if you would like to request a free sample. Please call our European Support office toll-free at 00800 4573 8000, email europe@scbt.com, or contact us by live chat.
Answered by: Technical Support
Date published: 2017-08-21

Dear Sir/Madam, I am planning to do ChIP experiment for PLZF protein. So I am wondering is it possible to get the same antibody in more concentrated form (200ug/0.1ml) like you have fo other antibodies. Thanks, Waiting for your reply, Sathish

Asked by: Satsa
Thank you for your question. The more concentrated X-form (200 µg/0.1 ml) of this PLZF Antibody (D-9): sc-28319 is available as a special order. Please contact our Special Orders Department to inquire about the details. Please call our European Support office toll-free at 00800 4573 8000, or e-mail specialorders@scbt.com.
Answered by: Technical Support
Date published: 2017-03-20

What is the recommended detection reagent for this mouse primary antibody since SCBT no longer sells traditional secondary antibodies?

Asked by: DefinitelyNotMatt
Thank you for your question. As an alternative to conventional secondary antibodies, use our great Mouse Binding Proteins! They are recombinant proteins that detect and bind to mouse antibodies; they come conjugated to HRP for WBs as well as many other conjugates for uses in different applications. https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety Please contact Technical Service if you have further questions concerning this product.
Answered by: Tech Service 9
Date published: 2016-12-29
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Rated 5 out of 5 by from Renin modulates HIV replication in T cells renin affects HIV -associated nephropathy. This antibody was very useful and provided great information for the publication.
Date published: 2017-07-25
Rated 5 out of 5 by from This antibody's effect on WB is very good . This antibody very cost-effective ,next time will continue to buy.
Date published: 2017-04-26
Rated 5 out of 5 by from Great features! clean and got the right binds for WB. validated by KO
Date published: 2017-04-11
Rated 5 out of 5 by from Good descripition The clear result got with cervical carcinoma cell PLZF by WB at 1:1000 dilution.
Date published: 2017-03-28
Rated 5 out of 5 by from 抗体非常好用,功能很好 这个抗体非常好用,特异性非常好,我们实验室做的是小鼠睾丸组织,染色非常好,值得推荐,免疫荧光非常特异。
Date published: 2017-02-28
Rated 5 out of 5 by from acceptable Endogenous and overexpressed PLZF were detected in Western blot, and the effect was acceptable
Date published: 2017-01-17
Rated 5 out of 5 by from Great for Western Blot Great detection of both endogenous and over-expressed PLZF in Western Blot.
Date published: 2017-01-03
Rated 5 out of 5 by from This has worked well in WB with mouse F9 cell This has worked well in WB with mouse F9 cell.
Date published: 2015-11-30
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