Plasmid Transfection Medium Reduced-serum medium suitable for transfections with DNA Plasmids. Recommended for use with Plasmid Transfection Reagent (sc-108061) and suspension of DNA for transfection.

Plasmid Transfection Medium

Plasmid Transfection Medium is rated 4.8 out of 5 by 5.
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Datasheets
    • 20 ml
    • Reduced-serum medium suitable for transfections with DNA Plasmids
    • Recommended for use with Plasmid Transfection Reagent (sc-108061) and suspension of DNA for transfection including: shRNA Plasmids, CRISPR/Cas9 KO Plasmids, HDR Plasmids or Cre Vector
    • Modification of Eagle's Minimal Essential Medium, buffered with HEPES and sodium bicarbonate, and supplemented with hypoxanthine, thymidine, sodium pyruvate, L-glutamine, trace elements and growth factors; protein level is minimal with insulin and transferrin being the only protein supplements; phenol red included at a reduced concentration as a pH indicator
    • Store at 4° C; light sensitive
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How much transfection medium (sc-108062) needed to prepare solution A when co-transfecting? For example: Plasmid A 1ug +Plasmid B1ug + 150ul transfection medium or Plasmid A 1ug +Plasmid B + 300ul transfection medium?

Asked by: Hope
Thank you for your question. If you are referring to our CRISPR/Cas9 KO Plasmid and HDR Plasmid co-transfection protocol (below) then the final volume is 150 µl after combining 1-3 µg of both plasmids. https://www.scbt.com/scbt/resources/protocols/crispr-cas9-ko-plasmid-and-hdr-plasmid-transfection
Answered by: Technical Support
Date published: 2018-01-31
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Rated 4 out of 5 by from High effciency Conventional liposome transfection may perform well for the robust cell lines, but the primary cell lines was not. The primary cell lines could be transfected by nucleofection, but the price of the nucleofection was so expensive. But, used the Plasmid Transfection Medium with liposome transfection was useful for the primary cell lines. Important ,the price was good!
Date published: 2016-12-03
Rated 5 out of 5 by from Good quality The quality is good and it worked well in our experiment. Really good!
Date published: 2016-12-02
Rated 5 out of 5 by from I used this for tranfection of Double nickase I used this for tranfection of Double nickase plasmids in human cells and it has good quality.
Date published: 2015-10-22
Rated 5 out of 5 by from Worked well in our CRISPR transfection Worked well in our CRISPR transfection.
Date published: 2015-09-01
Rated 5 out of 5 by from Works well Works well, use it all the time.
Date published: 2015-03-25
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