PIPES, Free Acid A buffering agent with a pKa near physiological pH

PIPES, Free Acid (CAS 5625-37-6)

PIPES, Free Acid | CAS 5625-37-6 is rated 5.0 out of 5 by 2.
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Synonym: 1,4-Piperazinediethanesulfonic acid; Piperazine-N,N'-bis(2-ethanesulfonic acid); 2,2'-(Piperazine-1,4-diyl)diethanesulfonic acid
Application: A buffering agent with a pKa near physiological pH
CAS Number: 5625-37-6
Purity: ≥99%
Molecular Weight: 302.4
Molecular Formula: C8H18N2O6S2
* Refer to Certificate of Analysis for lot specific data (including water content).
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PIPES [piperazine-N,N′-bis(2-ethanesulfonic acid)] is frequently used as a buffering agent in biochemistry. It is an ethanesulfonic acid buffer developed by Good et al. in the 1960s. PIPES has a pKa near the physiological pH which makes it useful in cell culture work. It has been documented to minimize lipid loss when buffering glutaraldehyde histology in plant and animal tissues.
Additional forms available: PIPES, Sesquisodium Salt (sc-216100); PIPES dipotassium salt (sc-258019); PIPES disodium salt (sc-264078); PIPES, Sodium Salt (sc-296097). Buffers can be prepared by adding a solution of base to PIPES free acid, titrating to the appropriate pH, or by mixing equimolar solutions of the monosodium salt and the disodium salt, titrating to the appropriate pH.


References

1. Good, N.E., et al. 1966. Biochemistry. 5: 467-477. PMID: 5942950
2. Schiff, R.I., et al. 1979. Scan Electron Microsc. 449-458. PMID: 118516
3. Yamamoto, K., et al. 1983. Histochemistry. 77: 339-351. PMID: 6305886
4. Blanchard, J.S. 1984. Meth. Enzymol. 104: 404-414. PMID: 6717292

Usage :
• This product is a high quality reagent, but has not been evaluated for use in cell culture. Please employ appropriate contaminant testing and/or sterilization techniques for the intended application.

• PIPES free acid is not very soluble in water (only 1 g per L at 100°C)1, but its salts are very soluble in water at the pH normally used as a buffer. PIPES will form a clear, colorless solution in 1 N NaOH, and is soluble to 20% (w/w) in 1 N NaOH. PIPES becomes soluble as the pH rises above pH 7 (it is converted to the salt form).
Appearance :
Crystalline powder
Physical State :
Solid
Solubility :
Soluble in 1M NaOH (0.5 M at 20° C), water (~1 g/L at 100° C), and 0.5 N NaOH (0.1 M).
Storage :
Store at room temperature
Melting Point :
>300° C (lit.)(dec.)
Boiling Point :
499.21° C (Predicted)
Density :
1.51 g/cm3 (Predicted)
Refractive Index :
n20D 1.57 (Predicted)
pK Values :
pKa 1: < 3 at 25 ºC pKa 2: 6.8 at 25 ºC pKa: 6.66 at 37ºC
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
PubChem CID :
79723
Merck Index :
14: 7479
MDL Number :
MFCD00006159
EC Number :
227-057-6
Beilstein Registry :
817713
SMILES :
C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O

Download SDS (MSDS)

Certificate of Analysis

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Rated 5 out of 5 by from This chemical works well in our cell culture This chemical works well in our cell culture studies.
Date published: 2015-10-07
Rated 5 out of 5 by from Yamamoto et al Yamamoto et al. (PubMed ID 6305886) found that the PIPES buffer preserved alkaline phosphatase activity and proved to be a better vehicle for aldehyde fixatives in rat hepatocytes. -SCBT Publication Review
Date published: 2015-02-03
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