PIPES [piperazine-N,N′-bis(2-ethanesulfonic acid)] is frequently used as a buffering agent in biochemistry. It is an ethanesulfonic acid buffer developed by Good et al. in the 1960s. PIPES has a pKa near the physiological pH which makes it useful in cell culture work. It has been documented to minimize lipid loss when buffering glutaraldehyde histology in plant and animal tissues. Additional forms available: PIPES, Sesquisodium Salt (sc-216100); PIPES dipotassium salt (sc-258019); PIPES disodium salt (sc-264078); PIPES, Sodium Salt (sc-296097). Buffers can be prepared by adding a solution of base to PIPES free acid, titrating to the appropriate pH, or by mixing equimolar solutions of the monosodium salt and the disodium salt, titrating to the appropriate pH.
1. Good, N.E., et al. 1966. Biochemistry. 5: 467-477. PMID: 5942950
2. Schiff, R.I., et al. 1979. Scan Electron Microsc. 449-458. PMID: 118516
3. Yamamoto, K., et al. 1983. Histochemistry. 77: 339-351. PMID: 6305886
4. Blanchard, J.S. 1984. Meth. Enzymol. 104: 404-414. PMID: 6717292
• This product is a high quality reagent, but has not been evaluated for use in cell culture. Please employ appropriate contaminant testing and/or sterilization techniques for the intended application.
• PIPES free acid is not very soluble in water (only 1 g per L at 100°C)1, but its salts are very soluble in water at the pH normally used as a buffer. PIPES will form a clear, colorless solution in 1 N NaOH, and is soluble to 20% (w/w) in 1 N NaOH. PIPES becomes soluble as the pH rises above pH 7 (it is converted to the salt form).
Soluble in 1M NaOH (0.5 M at 20° C), water (~1 g/L at 100° C), and 0.5 N NaOH (0.1 M).
Store at room temperature
>300° C (lit.)(dec.)
499.21° C (Predicted)
1.51 g/cm3 (Predicted)
n20D 1.57 (Predicted)
pKa 1: < 3 at 25 ºC pKa 2: 6.8 at 25 ºC pKa: 6.66 at 37ºC
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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Rated 5 out of
This chemical works well in our cell cultureThis chemical works well in our cell culture studies.
Date published: 2015-10-07
Rated 5 out of
Yamamoto et alYamamoto et al. (PubMed ID 6305886) found that the PIPES buffer preserved alkaline phosphatase activity and proved to be a better vehicle for aldehyde fixatives in rat hepatocytes. -SCBT Publication Review
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