Phosphate Buffered Saline: powder for 5 L of 10X Ready to use for the preparation of 10X PBS; PBS solution is commonly used in biological research

Phosphate Buffered Saline: powder for 5 L of 10X

Phosphate Buffered Saline: powder for 5 L of 10X is rated 5.0 out of 5 by 1.
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Synonym: 10X PBS (powder)
Application: Ready to use for the preparation of 10X PBS; PBS solution is commonly used in biological research
* Refer to Certificate of Analysis for lot specific data (including water content).
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Phosphate Buffered Saline: powder for 5 L of 10X is ready to use for preparation of 5 L of concentrated 10X Phosphate-buffered saline (PBS). PBS buffer is ideal for maintaining a constant pH, and since it is isotonic and non-toxic to cells, it can be used in a wide variety of biological applications. PBS is frequently used as a wash and dilution buffer, and is also used to take a reference spectrum when measuring protein adsorption.
Additional sizes, formats, and concentrations available:
Phosphate Buffered Saline: 1 L of 1X (sc-362182)
Phosphate Buffered Saline: 100 ml of 10X (sc-362297)
Phosphate Buffered Saline: 500 ml of 10X (sc-362298)
Phosphate Buffered Saline: 1 L of 10X (sc-24946)
Phosphate Buffered Saline: 1 L of 20X (sc-362183)
Phosphate Buffered Saline: 5 L of 20X (sc-362299)
Phosphate Buffered Saline: powder for 100 ml of 10X (sc-362300)
Phosphate Buffered Saline: powder for 500 ml of 10X (sc-362301)
Phosphate Buffered Saline: powder for 1 L of 10X (sc-362302)


References

1. Dulbecco, R. and Vogt, M. 1954. J. Exp. Med. 99(2): 167-182. PMID: 13130792
2. Wilcox, C.S. et al. 1992. Proc. Natl. Acad. Sci. U.S.A. 89(24): 11993-11997. PMID: 1281548
3. Fernando, P.N. et al. 2001. J Chromatogr A. 920(1-2): 155-162. PMID: 11452994
4. Kang, J. et al. 2009. Anal. Biochem. 386(2): 251-255. PMID: 19150324
5. Andersson, J. and Dahlgren, U. 2010. J Dent Res. PMID: 20811070
6. Sambrook, Fritsch, and Maniatis (1989) Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, volume 3, appendix B.12

Usage :
• Reconstitute powder in ddH20, bringing final volume to 5 L (10X). Prior to use, dilute to a 1X solution and filter through a 0.22 µm filter into a sterile flask. Makes 50 L of 1X PBS buffer with a phosphate buffer concentration of 10mM, 2.7mM potassium chloride, 137mM sodium chloride, and 1.76mM potassium phosphate. The PBS solution pH will be approximately 7.4.
• If buffer is used in cell culture applications, sterilization of the solution may be necessary. Pre-diluted buffer can be dispensed into aliquots and sterilized by autoclaving (20 min, 121°C, liquid cycle).
• PBS can be stored at room temperature, but non-sterile solutions may warrant refrigeration to prevent bacterial growth over time. Concentrated stock solutions may precipitate when cooled and should be kept at room temperature until precipitate has completely dissolved before use.
Physical State :
Solid
pH :
diluted to a [1X] pH will be ~7.4
Storage :
Store at room temperature
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

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Phosphate Buffered Saline: powder for 5 L of 10X  Product Citations

See how others have used Phosphate Buffered Saline: powder for 5 L of 10X. Click on the entry to view the PubMed entry .

Citations 1 to 1 of 1 total

PMID: # 23946635  Joachim, SC. et al. 2013. Molecular vision. 19: 1804-14.

Citations 1 to 1 of 1 total

What is the protocol to make this 10X PBS from this product?

Asked by: Guest
Thank you for your question. This information can be found in the “Technical Information” tab on our website (under the Ordering Information). Usage: Reconstitute powder in ddH20, bringing final volume to 5 L (10X). Prior to use, dilute to a 1X solution and filter through a 0.22 µm filter into a sterile flask. Makes 50 L of 1X PBS buffer with a phosphate buffer concentration of 10mM, 2.7mM potassium chloride, 137mM sodium chloride, and 1.76mM potassium phosphate. The PBS solution pH will be approximately 7.4.
Answered by: Technical Support
Date published: 2019-04-13

For making 1L of 10x PBS, how many gram of PBS powder should I use ?

Asked by: H_ONE
Thank you for your question. This is provided as a ready to use powder for preparation of 5L of concentrated 10X PBS. If you only want to make 1L of prepared buffer, we recommend an alternative product, sc-24946: Phosphate Buffered Saline: 1 L of 10X.
Answered by: Technical Support
Date published: 2019-04-13

How many grams of phosphate buffered saline powder is in sc-24947?

Asked by: chemicalsmg
Thank you for your question. Phosphate Buffered Saline: powder for 5 L of 10X: sc-24947 contains approximately 557.0 g of Phosphate buffer saline powder. Approximate value may vary per lot.
Answered by: Chemical Support 3
Date published: 2019-02-23
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Rated 5 out of 5 by from Buy it routinely from you Buy it routinely from you, use in WB and no issues.
Date published: 2015-08-04
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